Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0001154 (prpE) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0001154 showshow history
Names
prpE yahU JW0326 ECK0332 b0335
Product
propionyl-CoA synthetase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 352706 354592 1887 629
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000873 (IPR000873 AMP-dependent synthetase/ligase) Apr 2008
Automated Process Approved IPR012694 (IPR012694 Propionate--CoA ligase) Apr 2008
Automated Process Approved IPR032387 (IPR032387 Acetyl-coenzyme A synthetase, N-terminal domain) Jul 2018
Automated Process Approved IPR025110 (IPR025110 AMP-binding enzyme, C-terminal domain) Jul 2018
Automated Process Approved PF00501 (PF00501 AMP-binding enzyme) Jul 2018
Automated Process Approved PF16177 (PF16177 Acetyl-coenzyme A synthetase N-terminus) Jul 2018
Automated Process Approved PF13193 (PF13193 AMP-binding enzyme C-terminal domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0019395 fatty acid oxidation Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0019629 propionate catabolic process, 2-methylcitrate cycle GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012694
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000873
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0005737 cytoplasm Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0050218 propionate-CoA ligase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012694
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000873
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name prpE Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK0332 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW0326 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym yahU Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved locus tag b0335 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product propionyl-CoA synthetase Published Annotation - EcoCyc Accession Number: G6200
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Approved alternate product name putative propionyl-CoA synthetase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name putative acetyl-CoA synthetase of the propionate catabolism operon Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name predicted propionyl-CoA synthetase with ATPase domain Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved alternate product name propionate--CoA ligase Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Protein Family
Automated Process Approved protein family IPR032387 Acetyl-coenzyme A synthetase, N-terminal domain Protein Sequence Similarity - InterPro Domain: IPR032387
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR025110 AMP-binding enzyme, C-terminal domain Protein Sequence Similarity - InterPro Domain: IPR025110
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00501 AMP-binding enzyme Protein Sequence Similarity - PFAM Model Name: PF00501
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR012694 Propionate--CoA ligase Protein Sequence Similarity - InterPro Domain: IPR012694
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR000873 AMP-dependent synthetase/ligase Protein Sequence Similarity - InterPro Domain: IPR000873
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF16177 Acetyl-coenzyme A synthetase N-terminus Protein Sequence Similarity - PFAM Model Name: PF16177
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF13193 AMP-binding enzyme C-terminal domain Protein Sequence Similarity - PFAM Model Name: PF13193
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 6.2.1.17 Published Annotation - Swiss-Prot Accession Number: P77495 Feb 2006
Person Uncurated MultiFun 1.1.2 (metabolism; carbon utilization; fatty acids) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.1 (location of gene products; cytoplasm) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P77495 Published Annotation - Swiss-Prot Accession Number: P77495 Jan 2005
Automated Process Approved db xref GeneID:946891 Published Annotation - Entrez Gene Database ID: 946891
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:1786529 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P77495 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0001154 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG13604 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function putative enzyme; Not classified Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note putative propionyl-CoA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0019395 - fatty acid oxidation Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC73438.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000873
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0019629 propionate catabolic process, 2-methylcitrate cycle GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012694
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0019395 fatty acid oxidation Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0005737 cytoplasm Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0050218 propionate-CoA ligase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012694
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000873
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0006625 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003319 prpE CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003795 prpE CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003144 prpE CDS
Ortholog
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0001106 prpE CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0001618 prpE CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0000554 prpE CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0007482 prpE CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0000414 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0000331 prpE CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003971 prpE CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0009297 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0007049 prpE CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005768 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0076529 prpE CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0000395 prpE CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0000484 prpE CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0007039 prpE CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008062 prpE CDS
Automated Process Uncurated Go view Escherichia coli E2348/69 (EPEC) BCI-0000313 prpE CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0004809 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0003901 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0010578 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0008161 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0009238 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007332 prpE CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0005245 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007957 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0008976 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007867 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0009140 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007582 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007439 prpE CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008940 prpE CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0024320 prpE CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0008726 acs_1 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005082 prpE CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0000338 prpE CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0000485 prpE CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0000569 prpE CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0000480 prpE CDS
Person Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0174548 prpE pseudogene
Automated Process Approved Go view Escherichia coli SE11 AFS-0000368 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0000365 prpE CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0130279 prpE CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004953 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0000367 prpE CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0004583 prpE CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0002686 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0000482 prpE CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0004681 prpE CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0001199 prpE CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0001178 prpE CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0005770 prpE CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0004831 prpE CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0004187 prpE CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0004692 prpE CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006732 prpE CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0008851 prpE CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0004781 prpE CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0006919 prpE CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0002370 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0002352 prpE CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0003239 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0005071 prpE CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0007042 prpE CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0004513 prpE CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0006730 prpE CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0106079 prpE CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0084208 prpE CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0000435 prpE CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0091709 prpE CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0009130 prpE CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0005467 prpE CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006454 prpE CDS
Person Uncurated Go view Vibrio cholerae N16961 ADH-0001412 VC1340 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20158mutantTn5 InsertionRequest the StrainABE-0001154prpEprpE::Tn5KAN-I-SceI at position 398 in Minus orientation ,- pKD465Blattner Laboratory
FB20159mutantTn5 InsertionRequest the StrainABE-0001154prpEprpE::Tn5KAN-I-SceI at position 398 in Minus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 347706 to 359592
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0001133prpRCDScomplement346857348443Upstream
ABE-0001138prpBCDSforward348682349572Upstream
ABE-0285348repeat_regionforward349605349969Upstream
ABE-0001148prpCCDSforward350012351181Upstream
ABE-0001152prpDCDSforward351215352666Upstream
ABE-0001154prpECDSforward352706354592Matches
ABE-0285349repeat_regionforward354599354769Downstream
ABE-0001159codBCDSforward354922356181Downstream
ABE-0001161codACDSforward356171357454Downstream
ABE-0285350repeat_regionforward357484357562Downstream
ABE-0285351repeat_regionforward357684357762Downstream
ABE-0001165cynRCDScomplement357791358690Downstream
ABE-0001171cynTCDSforward358799359458Downstream
ABE-0001173cynSCDSforward359489359959Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.