Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0001196 (mhpA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0001196 showshow history
Names
mhpA JW0338 ECK0344 b0347
Product
3-(3-hydroxyphenyl)propionate hydroxylase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 368611 370275 1665 555
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001327 (IPR001327 (DEPRECATED) Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain) Apr 2008
Automated Process Approved IPR002938 (IPR002938 FAD-binding domain) Apr 2008
Automated Process Approved IPR003042 (IPR003042 (DEPRECATED) Aromatic-ring hydroxylase-like) Apr 2008
Automated Process Approved IPR036188 (IPR036188 FAD/NAD(P)-binding domain superfamily) Jul 2018
Automated Process Approved PF01494 (PF01494 FAD binding domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0019395 fatty acid oxidation Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006725 cellular aromatic compound metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002938
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003042
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002938
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0071949 FAD binding GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR002938
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0004497 monooxygenase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002938
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0050660 flavin adenine dinucleotide binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001327
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001327
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name mhpA Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK0344 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW0338 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b0347 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product 3-(3-hydroxyphenyl)propionate hydroxylase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name 3-(3-hydroxy-phenyl)propionate hydroxylase, FAD/NAD(P)-binding Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name 3-(3-hydroxyphenyl)propionate hydroxylase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family PF01494 FAD binding domain Protein Sequence Similarity - PFAM Model Name: PF01494
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR003042 (DEPRECATED) Aromatic-ring hydroxylase-like Protein Sequence Similarity - InterPro Domain: IPR003042
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR036188 FAD/NAD(P)-binding domain superfamily Protein Sequence Similarity - InterPro Domain: IPR036188
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR001327 (DEPRECATED) Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain Protein Sequence Similarity - InterPro Domain: IPR001327
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR002938 FAD-binding domain Protein Sequence Similarity - InterPro Domain: IPR002938
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Approved EC number 1.14.13.- Published Annotation - Swiss-Prot Accession Number: P77397 Feb 2006
Person Uncurated MultiFun 1.1.2 (metabolism; carbon utilization; fatty acids) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0001196 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG20273 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P77397 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1786543 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945197 Published Annotation - Entrez Gene Database ID: 945197
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P77397 Published Annotation - Swiss-Prot Accession Number: P77397 Jan 2005
Person Approved function enzyme; Degradation of small molecules: Carbon compounds Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note GO_process: GO:0019395 - fatty acid oxidation Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC73450.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006725 cellular aromatic compound metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002938
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0019395 fatty acid oxidation Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003042
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002938
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0004497 monooxygenase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002938
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0071949 FAD binding GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR002938
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0050660 flavin adenine dinucleotide binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001327
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001327
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0004654 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003580 mhpA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003878 mhpA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0006217 mhpA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003398 mhpA CDS
Ortholog
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0001632 visC_1 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0008206 mhpA CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0006168 mhpA CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0009284 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0010224 mhpA CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009968 CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0000407 mhpA CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0000496 mhpA CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008978 mhpA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0004821 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000375 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0010594 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0008176 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0010780 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007908 mhpA CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0005261 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0010475 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0008992 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007883 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007711 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007077 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0010313 mhpA CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0010749 mhpA CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0024337 mhpA CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005093 mhpA CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0000351 mhpA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0000350 mhpA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0000605 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0000556 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0000467 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0000381 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0000377 mhpA CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0130293 mhpA CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004968 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0002128 mhpA CDS
Person Approved Go view Shigella sonnei 046 ADD-0000318 mhpA CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20162mutantTn5 InsertionRequest the StrainABE-0001196mhpAmhpA::Tn5KAN-I-SceI at position 1626 in Minus orientation ,+ pKD460Blattner Laboratory
FB20163mutantTn5 InsertionRequest the StrainABE-0001196mhpAmhpA::Tn5KAN-I-SceI at position 1626 in Minus orientation ,- pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 363611 to 375275
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0001183lacZCDScomplement363231366305Upstream
ABE-0001189lacICDScomplement366428367510Upstream
ABE-0001191mhpRCDScomplement367587368420Upstream
ABE-0001196mhpACDSforward368611370275Matches
ABE-0001198mhpBCDSforward370277371221Downstream
ABE-0001201mhpCCDSforward371239372105Downstream
ABE-0001205mhpDCDSforward372115372924Downstream
ABE-0001207mhpFCDSforward372921373871Downstream
ABE-0001209mhpECDSforward373868374881Downstream
ABE-0285354repeat_regionforward374930375365Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.