Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0001219 (frmB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0001219 showshow history
Names
frmB JW0346 ECK0352 yaiM b0355
Product
S-formylglutathione hydrolase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 377535 378368 834 278
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR014186 (IPR014186 S-formylglutathione hydrolase) Apr 2008
Automated Process Approved IPR000801 (IPR000801 Esterase-like) Apr 2008
Automated Process Approved IPR029058 (IPR029058 Alpha/Beta hydrolase fold) Jul 2018
Automated Process Approved PF00756 (PF00756 Putative esterase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0046294 formaldehyde catabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR014186
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO molecular function GO:0018738 S-formylglutathione hydrolase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR014186
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Automated Process Approved name frmB Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym yaiM Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Feb 2004
Person Approved synonym JW0346 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK0352 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b0355 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product S-formylglutathione hydrolase Published Annotation in EcoGene - EG Number: EG13295
Reference: Escherichia coli K-12 MG1655 		Apr 2012
Person Uncurated alternate product name predicted esterase Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 		Apr 2012
Person Approved alternate product name putative S-formylglutathione hydrolase Unpublished Sequence Analysis - Author Name, Email; Comment: Christopher D. Herring, chris@genome.wisc.edu Jan 2006
Protein Family
Automated Process Approved protein family IPR014186 S-formylglutathione hydrolase Protein Sequence Similarity - InterPro Domain: IPR014186
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR000801 Esterase-like Protein Sequence Similarity - InterPro Domain: IPR000801
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00756 Putative esterase Protein Sequence Similarity - PFAM Model Name: PF00756
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR029058 Alpha/Beta hydrolase fold Protein Sequence Similarity - InterPro Domain: IPR029058
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 3.1.2.12 Protein Sequence Similarity - InterPro Domain: IPR014186
Reference: Escherichia coli K-12 MG1655 		Dec 2008
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG13295 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P51025 Published Annotation - Swiss-Prot Accession Number: P51025 Jan 2005
Automated Process Approved db xref ASAP:ABE-0001219 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P51025 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1786551 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:944991 Published Annotation - Entrez Gene Database ID: 944991
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved function enzyme: Formaldehyde degradation Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved note putative S-formylglutathione hydrolase Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC73458.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0046294 formaldehyde catabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR014186
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO molecular function GO:0018738 S-formylglutathione hydrolase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR014186
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0004026 frmB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0000084 yeiG CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003476 frmB CDS
Ortholog
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0000551 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0008198 fghA2 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0000424 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0006160 fghA CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0000339 yaiM CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003963 frmB CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0009275 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0010216 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009976 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0076538 frmB CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0000415 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0000504 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0007038 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008986 fghA2 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0000321 frmB CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0004830 fghA1 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000383 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0010602 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0008184 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0010788 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007916 fghA2 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0005269 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0010467 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0009000 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007891 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007719 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007085 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0010321 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0010757 fghA2 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0024345 frmB CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0010005 frmB CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005102 fghA2 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0000807 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0000356 frmB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0000358 frmB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0000597 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0000548 fghA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0000577 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0000460 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0174557 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0000389 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0000385 fghA2 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0130301 frmB CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004976 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0000376 yaiM CDS
Person Uncurated Go view Photorhabdus luminescens subsp. laumondii TTO1 [T] ACD-0146670 CDS
Automated Process Uncurated Go view Shigella boydii BS512 ADJ-0005666 fghA CDS
Person Approved Go view Shigella sonnei 046 ADD-0000362 frmB CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21994mutantTn5 InsertionRequest the StrainABE-0001219frmByaiM::Tn5KAN-2 at position 304 in Plus orientation ,+ pKD460Blattner Laboratory
FB23041mutantTn5 InsertionRequest the StrainABE-0001219frmByaiM::Tn5KAN-I-SceI at position 304 in Plus orientation ,- pKD465Tim Durfee (durf@genome.wisc.edu)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 372535 to 383368
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0001205mhpDCDSforward372115372924Downstream
ABE-0001207mhpFCDSforward372921373871Downstream
ABE-0001209mhpECDSforward373868374881Downstream
ABE-0285354repeat_regionforward374930375365Downstream
ABE-0001213mhpTCDSforward375459376670Downstream
ABE-0001216yaiLCDSforward376772377311Downstream
ABE-0285355repeat_regionforward377335377521Downstream
ABE-0001219frmBCDScomplement377535378368Matches
ABE-0001221frmACDScomplement378462379571Upstream
ABE-0001226frmRCDScomplement379606379881Upstream
ABE-0001230yaiOCDScomplement380069380842Upstream
ABE-0001232yaiX_2CDSpcomplement380844381059Upstream
ABE-0285111yaiXpseudogenecomplement380844381254(Interval #2) Upstream
ABE-0001235is_elementcomplement381260382590Upstream
ABE-0285356mobile_elementforward381260382590Upstream
ABE-0001237insC-1CDSforward381351381716Upstream
ABE-0001239insD-1CDSforward381674382579Upstream
ABE-0001241yaiX_1CDSpcomplement382504382872Upstream
ABE-0285111yaiXpseudogenecomplement382591382872(Interval #1) Upstream
ABE-0001243yaiPCDScomplement382739383935Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.