Escherichia coli K-12 MG1655
About ASAP Data Release Policy University of Wisconsin—Madison
Home    Overview    Annotations    BLAST    Downloads    Comparative Genomics    Experiments    Strains    Help (this script)    User: guest  / Log On   
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0001601 (acrB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0001601 showshow history
Names
acrB JW0451 ECK0456 acrE b0462
Product
multidrug efflux system protein
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 481254 484403 3150 1050
Sequence

Get full DNA or protein sequence

View sequence with flanking regions: bases upstream and bases downstream

Context

Browse sequence in GaPP

You last hovered over feature (none)
Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR004764 (IPR004764 Hydrophobe/amphiphile efflux-1 HAE1) Apr 2008
Automated Process Approved IPR001036 (IPR001036 Acriflavin resistance protein) Apr 2008
Automated Process Approved IPR027463 (IPR027463 Multidrug efflux transporter AcrB TolC docking domain, DN/DC subdomains) Jul 2018
Automated Process Approved PF00873 (PF00873 AcrB/AcrD/AcrF family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0042493 response to drug Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0006855 drug transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004764
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004764
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004764
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0015562 efflux transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004764
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004764
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0022857 transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name acrB Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW0451 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym acrE Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved synonym ECK0456 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b0462 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product multidrug efflux system protein Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name acridine efflux pump Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name acridine efflux pump (RND family) Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR027463 Multidrug efflux transporter AcrB TolC docking domain, DN/DC subdomains Protein Sequence Similarity - InterPro Domain: IPR027463
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00873 AcrB/AcrD/AcrF family Protein Sequence Similarity - PFAM Model Name: PF00873
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR001036 Acriflavin resistance protein Protein Sequence Similarity - InterPro Domain: IPR001036
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR004764 Hydrophobe/amphiphile efflux-1 HAE1 Protein Sequence Similarity - InterPro Domain: IPR004764
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 4.2.A.6 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Resistance-Nodulation-Cell Division (RND) Superfamily) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 5.6.4 (cell processes; protection; drug resistance/sensitivity) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.83 (transport; substrate; H+/acridine) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11704 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0001601 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1786667 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P31224 Published Annotation - Swiss-Prot Accession Number: P31224 Jan 2005
Automated Process Approved db xref UniProtKB/Swiss-Prot:P31224 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945108 Published Annotation - Entrez Gene Database ID: 945108
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved function transport; Drug/analog sensitivity Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note acridine efflux pump; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0042493 - response to drug Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC73564.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0006855 drug transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004764
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO biological process GO:0042493 response to drug Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004764
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004764
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004764
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0022857 transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO molecular function GO:0015562 efflux transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004764
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Homolog
Person Uncurated Go view Escherichia coli CFT073 (UPEC) ABR-0079141 acrD CDS
Person Uncurated Go view Escherichia coli CFT073 (UPEC) ABR-0080206 acrF CDS
Person Uncurated Go view Escherichia coli CFT073 (UPEC) ABR-0080501 mdtF CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0267128 mexF CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0268352 CDS
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0000006 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0000005 mdtF CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0000002 mdtF CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000001 mdtF CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0000002 mdtF CDS
Ortholog
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0001238 acrB_1 CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0001756 acrB_1 CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0019534 acrB CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0000527 CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0000434 acrB CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003857 acrB CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0009131 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0076655 acrB CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0000528 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0000625 CDS
Automated Process Uncurated Go view Escherichia coli E2348/69 (EPEC) BCI-0000418 acrB CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0004946 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0005391 acrB CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0024458 acrB CDS
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0008871 acrB_2 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005220 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0000694 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0000460 acrB CDS
Automated Process Approved Go view Escherichia coli K-12 W3110 ADY-0000464 acrB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0000658 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0000656 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0000602 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0000573 CDS
Person Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0174662 pseudogene
Automated Process Approved Go view Escherichia coli SE11 AFS-0000496 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0000504 CDS
Automated Process Approved Go view Escherichia coli Sakai (EHEC) ACA-0130407 acrB CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0000490 acrB CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0004831 acrB pseudogene
Person Uncurated Go view Kluyvera ascorbata ATCC 33433 [T] APJ-0005165 acrB pseudogene
Automated Process Approved Go view Salmonella Typhi CT18 ABX-0106301 acrB CDS
Automated Process Approved Go view Salmonella Typhimurium LT2 [T] ABU-0092025 acrB CDS
Automated Process Approved Go view Shigella boydii 227 ADV-0000392 acrB CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0005789 acrB CDS
Automated Process Approved Go view Shigella dysenteriae 197 ADU-0000509 acrB CDS
Automated Process Approved Go view Shigella flexneri 2457T ABQ-0071384 acrB CDS
Automated Process Approved Go view Shigella flexneri 301 ABT-0086765 acrB CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0000455 acrB CDS
Automated Process Approved Go view Shigella sonnei 046 ADD-0000485 acrB CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 476254 to 489403
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0001576ybaZCDScomplement475982476371Downstream
ABE-0001579ffsncRNAforward476448476561Downstream
ABE-0001582ybaACDSforward476672477025Downstream
ABE-0001584pdeBCDScomplement477067478617Downstream
ABE-0001587ylaCCDScomplement478781479251Downstream
ABE-0001591maaCDScomplement479367479918Downstream
ABE-0001594hhaCDScomplement480090480308Downstream
ABE-0001596tomBCDScomplement480334480708Downstream
ABE-0001601acrBCDScomplement481254484403Matches
ABE-0001603acrACDScomplement484426485619Upstream
ABE-0001608acrRCDSforward485761486408Upstream
ABE-0001613mscKCDSforward486536489898Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.