Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0001971 (cusA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0001971 showshow history
Names
cusA silA JW0564 ECK0567 ybdE b0575
Product
copper/silver export system RND permease
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 598714 601857 3144 1048
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR004763 (IPR004763 Cation efflux system CzcA/CusA/SilA/NccA/HelA/CnrA) Apr 2008
Automated Process Approved IPR001036 (IPR001036 Acriflavin resistance protein) Apr 2008
Automated Process Approved IPR027463 (IPR027463 Multidrug efflux transporter AcrB TolC docking domain, DN/DC subdomains) Jul 2018
Automated Process Approved PF00873 (PF00873 AcrB/AcrD/AcrF family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006812 cation transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004763
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004763
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0022857 transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0008324 cation transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004763
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name cusA Experimental - PubMed ID: 11566977
Reference: Escherichia coli 		Aug 2002
Person Approved synonym ECK0567 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym silA Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym JW0564 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ybdE Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved locus tag b0575 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product copper/silver export system RND permease Published Annotation - EcoCyc Accession Number: EG12367
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Approved alternate product name copper/silver efflux system, membrane component Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name putative copper transport protein Experimental - PubMed ID: 11566977
Reference: Escherichia coli 		Jan 2006
Person Uncurated alternate product name outer membrane copper (silver) and drug transport protein (RND family) Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR027463 Multidrug efflux transporter AcrB TolC docking domain, DN/DC subdomains Protein Sequence Similarity - InterPro Domain: IPR027463
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00873 AcrB/AcrD/AcrF family Protein Sequence Similarity - PFAM Model Name: PF00873
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR001036 Acriflavin resistance protein Protein Sequence Similarity - InterPro Domain: IPR001036
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR004763 Cation efflux system CzcA/CusA/SilA/NccA/HelA/CnrA Protein Sequence Similarity - InterPro Domain: IPR004763
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.2.A.6 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Resistance-Nodulation-Cell Division (RND) Superfamily) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.28 (transport; substrate; cation) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12367 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0001971 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945191 Published Annotation - Entrez Gene Database ID: 945191
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref UniProtKB/Swiss-Prot:P38054 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1786788 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P38054 Published Annotation - Swiss-Prot Accession Number: P38054 Jan 2005
Person Approved function putative transport; Not classified Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC73676.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0006812 cation transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004763
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004763
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0022857 transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001036
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0008324 cation transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004763
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Homolog
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0268258 CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0268575 CDS
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002225 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002417 cusA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002686 cusA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004054 cusA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002609 cusA CDS
Ortholog
Automated Process Uncurated Go view Brenneria salicis ATCC 15712 [T] AMK-0003580 CDS
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0004414 CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0003035 czcA CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0001364 cusA_1 CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0001902 cusA CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0007854 CDS
Person Uncurated Go view Dickeya dadantii 3937 ABF-0020553 cusA CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0001262 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0001284 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006519 cusA CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0000610 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0005955 cusA CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0000517 cusA CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003773 cusA CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0009031 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0007558 cusA CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009889 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0076737 cusA CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0000618 cusA CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0000724 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0007443 cusA CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0007059 cusA CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0000499 cusA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005040 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001659 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0010830 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0008410 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0005672 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0005938 cusA CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0005496 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007273 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0009229 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0006620 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0009597 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0005878 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0005880 cusA CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0006719 cusA CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0024560 cusA CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0007377 cusA CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005303 cusA CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0000870 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0000570 cusA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0000580 cusA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0000935 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0000855 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0000714 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0000655 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0174752 pseudogene
Automated Process Approved Go view Escherichia coli SE11 AFS-0000649 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0000592 cusA CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0130507 cusA CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0005252 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0000577 cusA CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0000958 czcA CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0004786 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0005730 CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0061637 cusA CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0000785 cusA CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0000368 cusA CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0004117 cusA CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0005969 CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0000377 CDS
Person Approved Go view Shigella boydii 227 ADV-0000474 ybdE CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0005071 cusA CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0071468 ybdE CDS
Person Approved Go view Shigella flexneri 301 ABT-0086836 ybdE CDS
Automated Process Uncurated Go view Shigella flexneri 8401 BAG-0000541 ybdE CDS
Person Approved Go view Shigella sonnei 046 ADD-0000569 cusA CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0000309 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0010417 CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0002008 cusA CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0001874 cusA CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0001416 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0004387 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0001207 cusA CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB23339mutantTn5 InsertionRequest the StrainABE-0001971cusAybdE::Tn5KAN-I-SceI at position 312 in Plus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 593714 to 606857
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0001946cusSCDScomplement593328594770Upstream
ABE-0001948cusRCDScomplement594760595443Upstream
ABE-0001958cusCCDSforward595600596973Upstream
ABE-0001967cusFCDSforward597131597463Upstream
ABE-0001969cusBCDSforward597479598702Upstream
ABE-0001971cusACDSforward598714601857Matches
ABE-0001976phePCDSforward601959603335Downstream
ABE-0001978ybdGCDScomplement603416604663Downstream
ABE-0001981nfsBCDScomplement604771605424Downstream
ABE-0001986ybdFCDScomplement605518605886Downstream
ABE-0001988ybdJCDScomplement605951606199Downstream
ABE-0001992ybdKCDScomplement606265607383Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.