Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0001981 (nfsB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0001981 showshow history
Names
nfsB dprA ntr JW0567 ECK0570 nfsI nfnB b0578
Product
NAD(P)H-dependent dihydropteridine reductase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 604771 605424 654 218
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000415 (IPR000415 Nitroreductase-like) Apr 2008
Automated Process Approved IPR029479 (IPR029479 Nitroreductase) Jul 2018
Automated Process Approved PF00881 (PF00881 Nitroreductase family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0042493 response to drug Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000415
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000415
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Uncurated name nfsB Published Sequence Analysis - PubMed ID: 8947835
Reference: Escherichia coli K-12 		Apr 2009
Person Approved synonym ECK0570 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW0567 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym dprA Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym nfsI Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Automated Process Approved synonym ntr Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym nfnB Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved locus tag b0578 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product NAD(P)H-dependent dihydropteridine reductase Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 		Oct 2021
Person Approved alternate product name oxygen-insensitive NAD(P)H nitroreductase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name dihydropteridine reductase; oxygen-insensitive NAD(P)H nitroreductase Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR000415 Nitroreductase-like Protein Sequence Similarity - InterPro Domain: IPR000415
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00881 Nitroreductase family Protein Sequence Similarity - PFAM Model Name: PF00881
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR029479 Nitroreductase Protein Sequence Similarity - InterPro Domain: IPR029479
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 1.5.1.34 Published Annotation - Swiss-Prot Accession Number: P38489 Feb 2006
Person Approved EC number 1.-.-.- Published Annotation - Swiss-Prot Accession Number: P38489 Feb 2006
Person Uncurated MultiFun 1.7.1 (metabolism; central intermediary metabolism; unassigned reversible reactions) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 5.6.4 (cell processes; protection; drug resistance/sensitivity) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1786792 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945778 Published Annotation - Entrez Gene Database ID: 945778
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref UniProtKB/Swiss-Prot:P38489 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0001981 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P38489 Published Annotation - Swiss-Prot Accession Number: P38489 Jan 2005
Automated Process Approved db xref EcoGene:EG20151 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Drug/analog sensitivity Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note oxygen-insensitive NAD(P)H nitroreductase; GO_process: GO:0042493 - response to drug Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC73679.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0042493 response to drug Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000415
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000415
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0006646 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002781 nfsB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002806 nfsB CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0003489 nfsB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002626 nfsB CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0002716 nfnB CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001297 CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0001380 nfnB CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0001919 nfnB CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0001591 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0000372 nfnB CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006516 nfnB CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0000613 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0005952 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0000520 nfnB CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003770 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0009028 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0007555 nfnB CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009886 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0076740 nfsB CDS
Person Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0000621 nfsB pseudogene
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0000727 nfnB CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0007446 nfnB CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0000502 nfsB CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005043 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001662 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0010833 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0008413 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0005675 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0005935 nfnB CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0005499 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007276 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0009232 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0006623 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0009600 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0005875 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0005883 nfnB CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0006722 nfnB CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0024563 nfnB CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0007380 nfsB CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005306 nfnB CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0000873 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0000573 nfnB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0000583 nfnB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0000932 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0000852 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0000711 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0000658 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0174755 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0000652 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0000595 nfnB CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0130510 nfnB CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0005255 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0000580 nfnB CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0000555 nfnB CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0003493 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0004795 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0002478 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0000722 nfnB CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0004950 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0001842 nfnB CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0001796 nfnB CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0005499 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0005055 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0004412 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0007846 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006028 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0004196 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0005001 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0007188 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0002170 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0002156 nfnB CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0002999 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0005291 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0006185 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0106499 nfnB CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0084004 nfnB CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0000675 nfnB CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0092299 nfnB CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0005256 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0004967 CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0267357 CDS
Person Approved Go view Shigella boydii 227 ADV-0000477 nfnB CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0005869 nfnB CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0005074 nfnB CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0000550 nfnB CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0071472 nfnB CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0000544 nfnB CDS
Person Approved Go view Shigella sonnei 046 ADD-0000572 nfnB CDS
Person Uncurated Go view Vibrio cholerae N16961 ADH-0003527 VCA0637 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0009705 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0001699 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20203mutantTn5 InsertionRequest the StrainABE-0001981nfsBnfnB::Tn5KAN-2 at position 461 in Plus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 599771 to 610424
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0001971cusACDSforward598714601857Downstream
ABE-0001976phePCDSforward601959603335Downstream
ABE-0001978ybdGCDScomplement603416604663Downstream
ABE-0001981nfsBCDScomplement604771605424Matches
ABE-0001986ybdFCDScomplement605518605886Upstream
ABE-0001988ybdJCDScomplement605951606199Upstream
ABE-0001992ybdKCDScomplement606265607383Upstream
ABE-0285379sokEncRNAforward607734607792Upstream
ABE-0047279hokECDSforward607836607988Upstream
ABE-0002004mobile_elementforward608007609351Upstream
ABE-0002007insL-2CDSforward608065609177Upstream
ABE-0002009entDCDScomplement609459610079Upstream
ABE-0285380repeat_regionforward610117610214Upstream
ABE-0002014fepACDScomplement610254612494Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.