Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0002828 (iaaA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0002828 showshow history
Names
iaaA spt ybiK JW0812 ECK0818 b0828
Product
L-asparaginase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 866568 867533 966 322
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000246 (IPR000246 Peptidase T2, asparaginase 2) Apr 2008
Automated Process Approved IPR029055 (IPR029055 Nucleophile aminohydrolases, N-terminal) Jul 2018
Automated Process Approved PF01112 (PF01112 Asparaginase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006516 glycoprotein catabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000246
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0009063 cellular amino acid catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0030288 outer membrane-bounded periplasmic space GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0016787 hydrolase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000246
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0004067 asparaginase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000246
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name iaaA Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK0818 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym spt Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym ybiK Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Approved synonym JW0812 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b0828 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product L-asparaginase Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved alternate product name Isoaspartyl peptidase Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Protein Family
Automated Process Approved protein family IPR029055 Nucleophile aminohydrolases, N-terminal Protein Sequence Similarity - InterPro Domain: IPR029055
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR000246 Peptidase T2, asparaginase 2 Protein Sequence Similarity - InterPro Domain: IPR000246
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF01112 Asparaginase Protein Sequence Similarity - PFAM Model Name: PF01112
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Automated Process Uncurated EC number 3.-.-.- Published Annotation - Database Name: EC2GO: GO:0016787
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved EC number 3.5.1.1 Published Annotation - Swiss-Prot Accession Number: P37595 Feb 2006
Person Uncurated MultiFun 1.1.3 (metabolism; carbon utilization; amino acids) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12407 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1787050 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945456 Published Annotation - Entrez Gene Database ID: 945456
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref UniProtKB/Swiss-Prot:P37595 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0002828 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P37595 Published Annotation - Swiss-Prot Accession Number: P37595 Jan 2005
Automated Process Approved note putative asparaginase; GO_process: GO:0009063 - cellular amino acid catabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC73915.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Uncurated regulation expression regulated by acetate; expression is reduced by acetate Experimental - PubMed ID: 11244055
Reference: Escherichia coli ATCC43888 (EHEC) 		Nov 2005
Automated Process Uncurated subcellular localization Periplasmic Predicted by PSORTb - PSORTb Final_Score: 9.44
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006516 glycoprotein catabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000246
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0009063 cellular amino acid catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0030288 outer membrane-bounded periplasmic space GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0016787 hydrolase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000246
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0004067 asparaginase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000246
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0000264 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002679 iaaA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002533 iaaA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002155 iaaA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002350 iaaA CDS
Ortholog
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0003156 CDS
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0002953 iaaA CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001153 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0001657 iaaA_1 CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0002178 iaaA CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0009150 CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0019454 iaaA CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0004117 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0001855 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0300126 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC BAA-2158 APN-0001439 ybiK CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0002261 ybiK CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0000054 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0007656 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0000848 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0005680 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0000733 iaaA CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003539 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008714 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0007773 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0006312 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077004 iaaA CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0000827 iaaA CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001061 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0007802 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008210 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0000823 iaaA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005345 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0285024 iaaA pseudogene
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0008935 iaaA CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005565 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001160 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0000796 iaaA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0000836 iaaA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001122 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001085 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001013 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0000913 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0174996 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0000910 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0000852 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0285022 iaaA pseudogene
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0000833 ybiK CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0000970 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0000863 ybiK CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0003757 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0004513 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0000604 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0001189 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0000634 ybiK CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0003573 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0001348 ybiK CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0002925 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0009029 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0003019 CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0004441 ybiK CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0060333 CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0000083 CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0001250 CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0000239 CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0009420 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0005066 CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0001001 ybiK CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0005221 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0002676 ybiK CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0002593 ybiK CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0008043 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0005342 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0004698 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0007299 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0005270 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0004478 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0005285 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0007817 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0001920 CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0001908 ybiK CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0002663 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0005571 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0005774 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0005087 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0006110 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0107030 ybiK CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0083756 ybiK CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0000992 ybiK CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0093102 ybiK CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0008587 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0006358 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006350 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0004262 CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0265695 CDS
Person Approved Go view Shigella boydii 227 ADV-0000782 ybiK CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0007931 CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0000853 ybiK CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0071828 ybiK CDS
Person Approved Go view Shigella flexneri 301 ABT-0087134 ybiK CDS
Automated Process Uncurated Go view Shigella flexneri 8401 BAG-0000855 ybiK CDS
Person Approved Go view Shigella sonnei 046 ADD-0000871 iaaA CDS
Person Approved Go view Vibrio cholerae N16961 ADH-0002702 VC2603 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0010735 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0004557 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22027mutantTn5 InsertionRequest the StrainABE-0002828iaaAybiK::Tn5KAN-I-SceI at position 464 in Plus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 861568 to 872533
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0002810ybiWCDScomplement860174862606Upstream
ABE-0002812ybiYCDScomplement862612863511Upstream
ABE-0002818fsaACDSforward863642864304Upstream
ABE-0285407repeat_regionforward864333864370Upstream
ABE-0002820moeBCDScomplement864380865129Upstream
ABE-0002823moeACDScomplement865129866364Upstream
ABE-0002828iaaACDSforward866568867533Matches
ABE-0002830gsiACDSforward867520869391Overlaps Downstream
ABE-0002832gsiBCDSforward869411870949Downstream
ABE-0002834gsiCCDSforward870967871887Downstream
ABE-0002836gsiDCDSforward871890872801Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.