Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0002832 (gsiB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0002832 showshow history
Names
gsiB JW5111 ECK0820 yliB b0830
Product
periplasmic solute-binding component of a glutathione transporter
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 869411 870949 1539 513
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000914 (IPR000914 Solute-binding protein family 5 domain) Apr 2008
Automated Process Approved PF00496 (PF00496 Bacterial extracellular solute-binding proteins, family 5 Middle) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000914
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0030288 outer membrane-bounded periplasmic space GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0042597 periplasmic space Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000914
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name gsiB Published Annotation - PubMed ID: 16109926
Reference: Escherichia coli K-12 		Sep 2007
Person Approved synonym yliB Published Annotation - Swiss-Prot Accession Number: P75797 Aug 2002
Person Approved synonym JW5111 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK0820 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b0830 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product periplasmic solute-binding component of a glutathione transporter Experimental - PubMed ID: 16109926
Reference: Escherichia coli K-12 		Sep 2007
Automated Process Approved alternate product name glutathione periplasmic binding protein, ABC superfamily transporter Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Protein Family
Automated Process Approved protein family PF00496 Bacterial extracellular solute-binding proteins, family 5 Middle Protein Sequence Similarity - PFAM Model Name: PF00496
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR000914 Solute-binding protein family 5 domain Protein Sequence Similarity - InterPro Domain: IPR000914
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 4.S.152 (transport; substrate; peptide) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.3.A.1.p (transport; Primary Active Transporters; Pyrophosphate Bond (ATP, GTP, P2) Hydrolysis-driven Active Transporters; The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases; ABC superfamily, periplasmic binding component) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.2 (location of gene products; periplasmic space) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG13473 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P75797 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945459 Published Annotation - Entrez Gene Database ID: 945459
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref ASAP:ABE-0002832 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1787052 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P75797 Published Annotation - Swiss-Prot Accession Number: P75797 Jan 2005
Automated Process Approved note putative transport protein; GO_component: GO:0042597 - periplasmic space Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC73917.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Periplasmic Predicted by PSORTb - PSORTb Final_Score: 9.94
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000914
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0042597 periplasmic space Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0030288 outer membrane-bounded periplasmic space GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000914
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002769 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002247 gsiB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002082 gsiB CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0001652 gsiB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0001718 gsiB CDS
Ortholog
Automated Process Uncurated Go view Brenneria salicis ATCC 15712 [T] AMK-0001266 CDS
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0001186 CDS
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0002951 gsiB CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001155 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0001659 gsiB CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0002180 gsiB CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0009144 CDS
Person Uncurated Go view Dickeya dadantii 3937 ABF-0019463 CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0004112 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0001857 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0300137 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC BAA-2158 APN-0001441 yliB CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0002259 yliB CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0000052 gsiB CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0007658 gsiB CDS
Automated Process Uncurated Go view Escherichia coli 536 (UPEC) AEF-0000851 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0005678 gsiB CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003537 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008711 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0007771 gsiB CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0006314 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077006 gsiB CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0000829 gsiB CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001064 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0007800 gsiB CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008208 gsiB CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0000825 gsiB CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005347 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0002044 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0009683 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0008733 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0005999 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0008122 gsiB CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0005821 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0008953 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0009563 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0008561 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0006769 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0008888 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007600 gsiB CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0007360 gsiB CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0024877 CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0008937 gsiB CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005567 gsiB CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001158 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0000798 gsiB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0000838 yliB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001124 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001087 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001011 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0000915 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0174998 pseudogene
Automated Process Approved Go view Escherichia coli SE11 AFS-0000912 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0000854 gsiB CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0130822 CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0005570 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0000835 CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0000972 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0000865 yliB CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0003759 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0004518 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0000606 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0001191 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0000636 yliB CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0003571 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0001350 yliB CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0002923 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0009031 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0003021 CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0004443 ylib1 CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0063107 CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0001660 CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0003694 CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0004380 CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0006262 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0005068 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0002182 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0001004 yliB CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0005223 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0002688 yliB CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0002604 yliB CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0008041 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0005344 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0004700 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0007301 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0005272 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0004480 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0005287 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0007819 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0001918 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0001906 yliB CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0002661 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0005573 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0005772 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0005089 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0006112 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0107040 yliB CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0083754 yliB CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0000994 yliB CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0093108 yliB CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0008585 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0006356 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006352 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0004260 CDS
Person Approved Go view Shigella boydii 227 ADV-0000784 CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0007929 gsiB CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0000851 CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0071830 CDS
Person Approved Go view Shigella flexneri 301 ABT-0087136 CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0000857 CDS
Person Approved Go view Shigella sonnei 046 ADD-0000873 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0010758 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0004555 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22127mutantTn5 InsertionRequest the StrainABE-0002832gsiBb0830::Tn5KAN-I-SceI at position 752 in Minus orientation ,+ pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 864411 to 875949
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0002820moeBCDScomplement864380865129Upstream
ABE-0002823moeACDScomplement865129866364Upstream
ABE-0002828iaaACDSforward866568867533Upstream
ABE-0002830gsiACDSforward867520869391Upstream
ABE-0002832gsiBCDSforward869411870949Matches
ABE-0002834gsiCCDSforward870967871887Downstream
ABE-0002836gsiDCDSforward871890872801Downstream
ABE-0002839pdeICDSforward872979875327Downstream
ABE-0002841dgcICDSforward875335876663Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.