Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0003464 (pgaC) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0003464 showshow history
Names
pgaC JW1007 ECK1012 hmsR ycdQ b1022
Product
putative polysaccharide polymerase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1086521 1087846 1326 442
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001173 (IPR001173 Glycosyltransferase 2-like) Apr 2008
Automated Process Approved IPR023853 (IPR023853 Poly-beta-1,6 N-acetyl-D-glucosamine synthase PgaC/IcaA) Jul 2018
Automated Process Approved IPR029044 (IPR029044 Nucleotide-diphospho-sugar transferases) Jul 2018
Automated Process Approved PF00535 (PF00535 Glycosyl transferase family 2) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0042710 biofilm formation GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR023853
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Approved GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0008375 acetylglucosaminyltransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR023853
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Uncurated name pgaC Published Sequence Analysis - PubMed ID: 15090514
Reference: Escherichia coli K-12 MG1655 		Jan 2007
Person Approved synonym ycdQ Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW1007 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK1012 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym hmsR Published Annotation - Database Name: GenProtEC Jul 2002
Person Approved locus tag b1022 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product putative polysaccharide polymerase Published Sequence Analysis - PubMed ID: 15090514
Reference: Escherichia coli K-12 MG1655 		Jan 2007
Automated Process Approved alternate product name biofilm PGA synthase PgaCD, catalytic subunit; poly-beta-1,6-N-acetyl-D-glucosamine synthase; c-di-GMP-stimulated activity and dimerization Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Uncurated alternate product name predicted inner membrane N-glycosyltransferase Published Sequence Analysis - PubMed ID: 15090514
Reference: Escherichia coli K-12 MG1655 		Jan 2007
Protein Family
Automated Process Approved protein family IPR001173 Glycosyltransferase 2-like Protein Sequence Similarity - InterPro Domain: IPR001173
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR023853 Poly-beta-1,6 N-acetyl-D-glucosamine synthase PgaC/IcaA Protein Sequence Similarity - InterPro Domain: IPR023853
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR029044 Nucleotide-diphospho-sugar transferases Protein Sequence Similarity - InterPro Domain: IPR029044
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00535 Glycosyl transferase family 2 Protein Sequence Similarity - PFAM Model Name: PF00535
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Automated Process Uncurated EC number 2.4.1.- Published Annotation - Database Name: EC2GO: GO:0008375
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved EC number 2.4.-.- Published Annotation - Swiss-Prot Accession Number: P75905 Feb 2006
Person Uncurated MultiFun 4.9.B.32 (transport; Transporters of Unknown Classification; Putative uncharacterized transport protein; The Putative Vectorial Glycosyl Polymerization (VGP) Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0003464 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945606 Published Annotation - Entrez Gene Database ID: 945606
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref EcoGene:EG13863 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P75905 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P75905 Published Annotation - Swiss-Prot Accession Number: P75905 Jan 2005
Automated Process Approved db xref GI:1787259 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved function enzyme; Surface polysaccharides and antigens Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Uncurated function polymerizes UDP-N-acetyl-D-glucosamine to form the poly-beta-1,6-N-acetyl-D-glucosamine (PGA) adhesin important for biofilm formation Published Annotation - PubMed ID: 15090514
Reference: Escherichia coli K-12 MG1655 		Jan 2007
Automated Process Approved note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74107.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0042710 biofilm formation GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR023853
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0008375 acetylglucosaminyltransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR023853
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0003998 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003369 pgaC CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003470 pgaC CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0005110 pgaC CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003653 pgaC CDS
Ortholog
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0002152 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0004696 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0007259 pgaC CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001030 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0005457 pgaC CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0000948 ycdQ CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003354 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008414 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0008039 pgaC CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077255 pgaC CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001110 pgaC CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0009428 pgaC CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008319 pgaC CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005585 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0005101 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0009973 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009011 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0007617 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0005536 pgaC CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006104 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0006373 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0009875 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0006306 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0006960 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0006383 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0006888 pgaC CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0007838 pgaC CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025329 pgaC CDS
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0007491 pgaC CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005814 pgaC CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001326 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0000982 pgaC CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001034 pgaC CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001326 pgaC CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001319 pgaC CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001276 pgaC CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001112 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175252 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001113 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131196 pgaC CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0000594 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001088 ycdQ CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0004562 CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0004414 CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0064790 hmsR CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0000833 hmsR CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0009635 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0002306 CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0267822 CDS
Automated Process Uncurated Go view Shigella flexneri 8401 BAG-0001095 ycdQ CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0005093 ycdQ CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0000385 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0007121 hmsR CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0002886 hmsR CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0000307 hmsR CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0000096 CDS
Person Approved Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0238365 hmsR CDS
Automated Process Uncurated Go view Yersinia pestis Angola (biovar Pestoides) ADI-0006256 pgaC CDS
Automated Process Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0001622 hmsR CDS
Automated Process Uncurated Go view Yersinia pestis B42003004 (biovar Antiqua) BAL-0004436 pgaC CDS
Automated Process Uncurated Go view Yersinia pestis CA88-4125 (biovar Orientalis) BAF-0001476 hmsR CDS
Person Approved Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0121794 hmsR CDS
Automated Process Uncurated Go view Yersinia pestis E1979001 (biovar Antiqua) BAM-0005371 pgaC CDS
Automated Process Uncurated Go view Yersinia pestis F1991016 (biovar Orientalis) BAQ-0007464 pgaC CDS
Automated Process Uncurated Go view Yersinia pestis FV-1 (biovar Orientalis) BAK-0000928 CDS
Automated Process Uncurated Go view Yersinia pestis IP275 (biovar Orientalis) BAC-0009146 pgaC CDS
Person Approved Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0032076 hmsR CDS
Automated Process Uncurated Go view Yersinia pestis MG05-1020 (biovar Orientalis) BAP-0006037 pgaC CDS
Automated Process Uncurated Go view Yersinia pestis Pestoides F (biovar Pestoides) BAI-0001393 CDS
Automated Process Uncurated Go view Yersinia pestis UG05-0454 (biovar Antiqua) BAN-0007230 pgaC CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0006102 pgaC CDS
Person Approved Go view Yersinia pseudotuberculosis IP32953 ACZ-0002031 hmsR CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0002372 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0002652 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22275mutantTn5 InsertionRequest the StrainABE-0003464pgaCycdQ::Tn5KAN-I-SceI at position 724 in Plus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1081521 to 1092846
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0285091efeUpseudogeneforward10813561082185Downstream
ABE-0003443efeU_2CDSpforward10814541082185Downstream
ABE-0003448efeOCDSforward10822431083370Downstream
ABE-0003451efeBCDSforward10833761084647Downstream
ABE-0285422repeat_regionforward10846821084767Downstream
ABE-0003458phoHCDSforward10849921086056Downstream
ABE-0003460pgaDCDScomplement10861061086519Downstream
ABE-0003464pgaCCDScomplement10865211087846Matches
ABE-0003467pgaBCDScomplement10878391089857Overlaps Upstream
ABE-0003469pgaACDScomplement10898661092289Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.