Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004024 (dhaM) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004024 showshow history
Names
dhaM JW5185 ptsD ECK1186 ycgC dhaH b1198
Product
dihydroxyacetone kinase phosphoryl donor subunit
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1247696 1249114 1419 473
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000032 (IPR000032 Phosphocarrier protein HPr-like) Apr 2008
Automated Process Approved IPR005698 (IPR005698 (DEPRECATED) Phosphotransferase system, phosphocarrier HPr protein) Apr 2008
Automated Process Approved IPR008731 (IPR008731 Phosphotransferase system, enzyme I N-terminal) Apr 2008
Automated Process Approved IPR008279 (IPR008279 PEP-utilising enzyme, mobile domain) Apr 2008
Automated Process Approved IPR012844 (IPR012844 Dihydroxyacetone kinase phosphotransferase subunit, N-terminal domain) Apr 2008
Automated Process Approved IPR004701 (IPR004701 Phosphotransferase system, mannose-type IIA component) Apr 2008
Automated Process Approved IPR001020 (IPR001020 Phosphotransferase system, HPr histidine phosphorylation site) Apr 2008
Automated Process Approved IPR035895 (IPR035895 HPr-like superfamily) Jul 2018
Automated Process Approved IPR036637 (IPR036637 Phosphohistidine domain superfamily) Jul 2018
Automated Process Approved IPR036662 (IPR036662 Phosphotransferase system, mannose-type IIA component superfamily) Jul 2018
Automated Process Approved IPR036618 (IPR036618 PtsI, HPr-binding domain superfamily) Jul 2018
Automated Process Approved PF03610 (PF03610 PTS system fructose IIA component) Jul 2018
Automated Process Approved PF05524 (PF05524 PEP-utilising enzyme, N-terminal) Jul 2018
Automated Process Approved PF00391 (PF00391 PEP-utilising enzyme, mobile domain) Jul 2018
Automated Process Approved PF00381 (PF00381 PTS HPr component phosphorylation site) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001020
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0016310 phosphorylation GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008279
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0009401 phosphoenolpyruvate-dependent sugar phosphotransferase system GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000032
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004701
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008731
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0016772 transferase activity, transferring phosphorus-containing groups GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008279
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005351 carbohydrate:proton symporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000032
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0008965 phosphoenolpyruvate-protein phosphotransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008731
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name dhaM Experimental - PubMed ID: 11350937
Reference: Escherichia coli 		Aug 2002
Person Approved synonym dhaH Published Annotation - Database Name: GenProtEC Jul 2002
Person Approved synonym ycgC Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK1186 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym ptsD Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym JW5185 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1198 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product dihydroxyacetone kinase phosphoryl donor subunit Experimental - PubMed ID: 11350937
Reference: Escherichia coli K-12 		Mar 2008
Automated Process Approved alternate product name fused predicted dihydroxyacetone-specific PTS enzymes: HPr component/EI component Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved alternate product name PTS hybrid protein Experimental - PubMed ID: 11350937
Reference: Escherichia coli 		Jan 2006
Person Uncurated alternate product name PTS system predicted dihydroxyacetone-specific HPr/EI component Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 		Mar 2008
Protein Family
Automated Process Approved protein family IPR008279 PEP-utilising enzyme, mobile domain Protein Sequence Similarity - InterPro Domain: IPR008279
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR012844 Dihydroxyacetone kinase phosphotransferase subunit, N-terminal domain Protein Sequence Similarity - InterPro Domain: IPR012844
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR005698 (DEPRECATED) Phosphotransferase system, phosphocarrier HPr protein Protein Sequence Similarity - InterPro Domain: IPR005698
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR000032 Phosphocarrier protein HPr-like Protein Sequence Similarity - InterPro Domain: IPR000032
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR004701 Phosphotransferase system, mannose-type IIA component Protein Sequence Similarity - InterPro Domain: IPR004701
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR008731 Phosphotransferase system, enzyme I N-terminal Protein Sequence Similarity - InterPro Domain: IPR008731
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR001020 Phosphotransferase system, HPr histidine phosphorylation site Protein Sequence Similarity - InterPro Domain: IPR001020
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR036618 PtsI, HPr-binding domain superfamily Protein Sequence Similarity - InterPro Domain: IPR036618
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR035895 HPr-like superfamily Protein Sequence Similarity - InterPro Domain: IPR035895
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR036637 Phosphohistidine domain superfamily Protein Sequence Similarity - InterPro Domain: IPR036637
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR036662 Phosphotransferase system, mannose-type IIA component superfamily Protein Sequence Similarity - InterPro Domain: IPR036662
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF03610 PTS system fructose IIA component Protein Sequence Similarity - PFAM Model Name: PF03610
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00381 PTS HPr component phosphorylation site Protein Sequence Similarity - PFAM Model Name: PF00381
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00391 PEP-utilising enzyme, mobile domain Protein Sequence Similarity - PFAM Model Name: PF00391
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF05524 PEP-utilising enzyme, N-terminal Protein Sequence Similarity - PFAM Model Name: PF05524
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Automated Process Uncurated EC number 2.7.-.- Published Annotation - Database Name: EC2GO: GO:0016772
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved EC number 2.7.1.- Published Annotation - Swiss-Prot Accession Number: P37349 Feb 2006
Person Uncurated MultiFun 4.8.A.7 (transport; Group Translocators; Accessory Factors Involved in Transport; The Phosphotransferase System Enzyme I (EI) Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 3.1.3.3 (regulation; type of regulation; posttranscriptional; inhibition / activation of enzymes) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.173 (transport; substrate; sugar) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12399 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0004024 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945749 Published Annotation - Entrez Gene Database ID: 945749
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P37349 Published Annotation - Swiss-Prot Accession Number: P37349 Jan 2005
Automated Process Approved db xref GI:226510935 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P37349 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved note PTS hybrid protein Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74282.4 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.97
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001020
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0009401 phosphoenolpyruvate-dependent sugar phosphotransferase system GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000032
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0016310 phosphorylation GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008279
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008731
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004701
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0008965 phosphoenolpyruvate-protein phosphotransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008731
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016772 transferase activity, transferring phosphorus-containing groups GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008279
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005351 carbohydrate:proton symporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000032
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003752 dhaM CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003519 dhaM CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002541 dhaM CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003260 dhaM CDS
Ortholog
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006060 dhaM CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001256 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009792 dhaM CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001148 ycgC CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003145 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008231 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005095 dhaM CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0008941 DhaM CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077767 dhaM CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001333 dhaM CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001647 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0009875 dhaM CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0010557 dhaM CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001405 dhaM CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005786 dhaM CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009951 dhaM CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0009803 dhaM CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025759 dhaM CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0005854 dhaM CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005979 dhaM CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001488 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001124 dhaM CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001214 dhaH CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001563 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001490 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001438 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001464 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175539 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001274 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001942 dhaM CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131655 dhaM CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004372 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001395 ycgC CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003544 dhaM CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0000247 dhaM CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0003988 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0000946 dhaM CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0003369 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0290643 dhaM CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0001616 CDS
Person Approved Go view Shigella boydii 227 ADV-0002077 ycgC CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006760 dhaM CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0007834 dhaM CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072338 ycgC CDS
Person Approved Go view Shigella flexneri 301 ABT-0087539 ycgC CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001287 ycgC CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0000021 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0001644 CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0003265 dhaM CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0002186 dhaM CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0003058 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22055mutantTn5 InsertionRequest the StrainABE-0004024dhaMycgC::Tn5KAN-I-SceI at position 1075 in Plus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1242696 to 1254114
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004002ldcACDScomplement12421661243080Downstream
ABE-0004006emtACDSforward12431801243791Downstream
ABE-0004009ycgRCDScomplement12437931244527Downstream
ABE-0004014ymgECDSforward12447281244982Downstream
ABE-0004019ycgYCDSforward12451601245600Downstream
ABE-0004021treACDScomplement12456791247376Downstream
ABE-0285435repeat_regionforward12476581247688Downstream
ABE-0004024dhaMCDScomplement12476961249114Matches
ABE-0004026dhaLCDScomplement12491251249757Upstream
ABE-0004029dhaKCDScomplement12497681250838Upstream
ABE-0004033dhaRCDSforward12510661252985Upstream
ABE-0004035ycgVCDScomplement12530851255952Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.