Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004029 (dhaK) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004029 showshow history
Names
dhaK ycgT ptsD JW5187 ECK1188 dhaK1 b1200
Product
dihydroxyacetone kinase subunit K
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1249768 1250838 1071 357
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR012736 (IPR012736 Dihydroxyacetone kinase DhaK, subunit 1) Apr 2008
Automated Process Approved IPR004006 (IPR004006 DhaK domain) Apr 2008
Automated Process Approved PF02733 (PF02733 Dak1 domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006071 glycerol metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004006
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0004371 glycerone kinase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004006
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name dhaK Experimental - PubMed ID: 11350937
Reference: Escherichia coli 		Aug 2002
Person Approved synonym JW5187 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK1188 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym dhaK1 Published Annotation - Database Name: GenProtEC Jul 2002
Automated Process Approved synonym ycgT Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved synonym ptsD Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved locus tag b1200 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product dihydroxyacetone kinase subunit K Published Annotation - EcoCyc Accession Number: G6627
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Automated Process Approved alternate product name dihydroxyacetone kinase, PTS-dependent, dihydroxyacetone-binding subunit Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved alternate product name dihydroxyacetone kinase, N-terminal domain Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family PF02733 Dak1 domain Protein Sequence Similarity - PFAM Model Name: PF02733
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR012736 Dihydroxyacetone kinase DhaK, subunit 1 Protein Sequence Similarity - InterPro Domain: IPR012736
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR004006 DhaK domain Protein Sequence Similarity - InterPro Domain: IPR004006
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Approved EC number 2.7.-.- Published Annotation - Swiss-Prot Accession Number: P76015 Feb 2006
Automated Process Uncurated EC number 2.7.1.29 Published Annotation - Database Name: EC2GO: GO:0004371
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated MultiFun 1.7.1 (metabolism; central intermediary metabolism; unassigned reversible reactions) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG13901 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P76015 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P76015 Published Annotation - Swiss-Prot Accession Number: P76015 Jan 2006
Automated Process Approved db xref GeneID:945747 Published Annotation - Entrez Gene Database ID: 945747
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:87081857 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0004029 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74284.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006071 glycerol metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004006
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0004371 glycerone kinase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004006
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0004139 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002516 dhaK CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0001980 dhaK CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002099 dhaK CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0001924 dhaK CDS
Ortholog
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002548 dhaK CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0003206 dhaK_1 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006062 dhaK CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001258 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009794 dhaK CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001150 dhaK CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003143 dhaK CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008229 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005097 dhaK CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0008939 DhaK CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077769 dhaK CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001335 dhaK CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001649 dhaK CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0009873 dhaK CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0010555 dhaK CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001407 dhaK CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005788 dhaK CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009953 dhaK CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0009805 dhaK CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025761 dhaK CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0005856 dhaK CDS
Automated Process Approved Go view Escherichia coli HS ADR-0005981 dhaK CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001486 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001126 dhaK CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001216 dhaK CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001561 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001492 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001440 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001466 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175541 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001276 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001940 dhaK CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131657 dhaK CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004370 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001397 ycgT CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0001179 dhaK CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003542 dhaK CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0000245 dhaK CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0003990 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0000944 dhaK CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0003371 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0010038 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0290645 dhaK CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0001614 dhaK CDS
Person Uncurated Go view Shigella boydii 227 ADV-0002075 CDS
Automated Process Uncurated Go view Shigella boydii BS512 ADJ-0006762 dhaK CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0007836 dhaK CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072340 CDS
Person Approved Go view Shigella flexneri 301 ABT-0087541 CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001289 CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0000019 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0001640 dhaK CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0003263 dhaK CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0002188 dhaK CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0003056 CDS
Automated Process Uncurated Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0234962 dAK1_1 CDS
Automated Process Uncurated Go view Yersinia pestis Angola (biovar Pestoides) ADI-0005055 CDS
Automated Process Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0003351 dhaK CDS
Automated Process Uncurated Go view Yersinia pestis B42003004 (biovar Antiqua) BAL-0007139 CDS
Automated Process Uncurated Go view Yersinia pestis CA88-4125 (biovar Orientalis) BAF-0004004 dhaK CDS
Automated Process Uncurated Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0126610 dhaK CDS
Automated Process Uncurated Go view Yersinia pestis E1979001 (biovar Antiqua) BAM-0006459 CDS
Automated Process Uncurated Go view Yersinia pestis F1991016 (biovar Orientalis) BAQ-0005950 CDS
Automated Process Uncurated Go view Yersinia pestis FV-1 (biovar Orientalis) BAK-0000616 CDS
Automated Process Uncurated Go view Yersinia pestis IP275 (biovar Orientalis) BAC-0005932 CDS
Automated Process Uncurated Go view Yersinia pestis K1973002 (biovar Mediaevalis) BAO-0004285 CDS
Person Uncurated Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0030477 dhaK CDS
Automated Process Uncurated Go view Yersinia pestis MG05-1020 (biovar Orientalis) BAP-0007394 CDS
Automated Process Uncurated Go view Yersinia pestis Nepal516 (biovar Antiqua) BAD-0000905 dhaK CDS
Automated Process Uncurated Go view Yersinia pestis Pestoides F (biovar Pestoides) BAI-0003602 CDS
Automated Process Uncurated Go view Yersinia pestis UG05-0454 (biovar Antiqua) BAN-0004783 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0007301 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0000824 dhaK CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0000960 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0004060 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22899mutantTn5 InsertionRequest the StrainABE-0004029dhaKb1200::Tn5(KAN-I-SceI) at position 606 in Plus orientation ,+ pKD46 0Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1244768 to 1255838
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004014ymgECDSforward12447281244982Downstream
ABE-0004019ycgYCDSforward12451601245600Downstream
ABE-0004021treACDScomplement12456791247376Downstream
ABE-0285435repeat_regionforward12476581247688Downstream
ABE-0004024dhaMCDScomplement12476961249114Downstream
ABE-0004026dhaLCDScomplement12491251249757Downstream
ABE-0004029dhaKCDScomplement12497681250838Matches
ABE-0004033dhaRCDSforward12510661252985Upstream
ABE-0004035ycgVCDScomplement12530851255952Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.