Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004111 (narK) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004111 showshow history
Names
narK JW1214 ECK1217 b1223
Product
nitrate/nitrite transporter
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1277957 1279348 1392 464
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR004737 (IPR004737 Nitrate transporter NarK/NarU-like) Apr 2008
Automated Process Approved IPR011701 (IPR011701 Major facilitator superfamily) Apr 2008
Automated Process Approved IPR036259 (IPR036259 MFS transporter superfamily) Jul 2018
Automated Process Approved PF07690 (PF07690 Major Facilitator Superfamily) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0015707 nitrite transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO biological process GO:0006807 nitrogen compound metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0015706 nitrate transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR011701
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0015698 inorganic anion transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0015113 nitrite transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0015103 inorganic anion transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0015112 nitrate transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name narK Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK1217 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW1214 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1223 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product nitrate/nitrite transporter Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name nitrite extrusion protein Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name nitrite extrusion protein (MFS family) Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR011701 Major facilitator superfamily Protein Sequence Similarity - InterPro Domain: IPR011701
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF07690 Major Facilitator Superfamily Protein Sequence Similarity - PFAM Model Name: PF07690
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR004737 Nitrate transporter NarK/NarU-like Protein Sequence Similarity - InterPro Domain: IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR036259 MFS transporter superfamily Protein Sequence Similarity - InterPro Domain: IPR036259
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated MultiFun 4.2.A.1 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Major Facilitator Superfamily (MFS)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.8.3 (metabolism; metabolism of other compounds; nitrogen metabolism) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.145 (transport; substrate; nitrite) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG10642 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P10903 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0004111 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P10903 Published Annotation - Swiss-Prot Accession Number: P10903 Jan 2005
Automated Process Approved db xref GI:1787475 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945783 Published Annotation - Entrez Gene Database ID: 945783
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved function transport; Transport of small molecules: Anions Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note nitrite extrusion protein; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006807 - nitrogen compound metabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74307.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0015707 nitrite transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO biological process GO:0006807 nitrogen compound metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0015706 nitrate transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR011701
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0015698 inorganic anion transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0015113 nitrite transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0015103 inorganic anion transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0015112 nitrate transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0001763 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002402 narK CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002320 narK CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000110 narK CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0000039 narK CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0001859 narK CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002506 narK CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0003167 narK CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0020265 narK CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001283 CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0001172 narK CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003118 narK CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008199 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077795 narK CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001361 narK CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001675 CDS
Automated Process Uncurated Go view Escherichia coli E2348/69 (EPEC) BCI-0001431 narK CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006547 narK CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025786 narK CDS
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0005882 narK CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006010 narK CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001462 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001149 narK CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001245 narK CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001531 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001522 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001473 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001492 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001304 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001915 narK CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131680 narK CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001424 narK CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0001550 narK CDS
Person Approved Go view Pectobacterium atrosepticum SCRI1043 ABL-0062291 narK CDS
Person Approved Go view Pectobacterium carotovorum WPP14 ADT-0003832 narK CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0002062 narK CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0001108 narK CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0107698 narK CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0083388 narK CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0095738 narK CDS
Person Approved Go view Shigella boydii 227 ADV-0002045 narK CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006794 narK CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0001455 narK CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072363 narK CDS
Person Approved Go view Shigella flexneri 301 ABT-0087564 narK CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001315 narK CDS
Person Approved Go view Shigella sonnei 046 ADD-0002146 narK CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0002269 nark CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20269mutantTn5 InsertionRequest the StrainABE-0004111narKnarK::Tn5KAN-2 at position 248 in Plus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1272957 to 1284348
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004089chaCCDSforward12725071273202Upstream
ABE-0004091ychNCDScomplement12732461273599Upstream
ABE-0004095ychOCDSforward12737841275178Upstream
ABE-0004097narLCDScomplement12751791275829Upstream
ABE-0004099narXCDScomplement12758221277618Upstream
ABE-0004111narKCDSforward12779571279348Matches
ABE-0285847narSncRNAforward12793371279520Overlaps Downstream
ABE-0004119narGCDSforward12798641283607Downstream
ABE-0004121narHCDSforward12836041285142Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.