Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004119 (narG) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004119 showshow history
Names
narG JW1215 ECK1218 narC chlC b1224
Product
nitrate reductase A subunit alpha
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1279864 1283607 3744 1248
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR006655 (IPR006655 Molybdopterin oxidoreductase, prokaryotic, conserved site) Apr 2008
Automated Process Approved IPR006468 (IPR006468 Nitrate reductase, alpha subunit) Apr 2008
Automated Process Approved IPR009010 (IPR009010 Aspartate decarboxylase-like domain superfamily) Apr 2008
Automated Process Approved IPR006657 (IPR006657 Molybdopterin dinucleotide-binding domain) Apr 2008
Automated Process Approved IPR006656 (IPR006656 Molybdopterin oxidoreductase) Apr 2008
Automated Process Approved IPR028189 (IPR028189 Nitrate reductase, alpha subunit, N-terminal) Jul 2018
Automated Process Approved IPR006963 (IPR006963 Molybdopterin oxidoreductase, 4Fe-4S domain) Jul 2018
Automated Process Approved PF01568 (PF01568 Molydopterin dinucleotide binding domain) Jul 2018
Automated Process Approved PF14710 (PF14710 Respiratory nitrate reductase alpha N-terminal) Jul 2018
Automated Process Approved PF00384 (PF00384 Molybdopterin oxidoreductase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006657, IPR006656, IPR006468, IPR006963
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0042126 nitrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006655
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0009325 nitrate reductase complex GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0043546 molybdopterin cofactor binding GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006657
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0030151 molybdenum ion binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006657
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0008940 nitrate reductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006655
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO molecular function GO:0009054 (DEPRECATED) electron acceptor activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Uncurated name narG Published Sequence Analysis - PubMed ID: 6094247
Reference: Escherichia coli K-12 		Jan 2007
Person Approved synonym chlC Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved synonym narC Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved synonym ECK1218 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW1215 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1224 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product nitrate reductase A subunit alpha Published Annotation - EcoCyc Accession Number: EG10638
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Approved alternate product name nitrate reductase 1, alpha subunit Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family PF14710 Respiratory nitrate reductase alpha N-terminal Protein Sequence Similarity - PFAM Model Name: PF14710
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00384 Molybdopterin oxidoreductase Protein Sequence Similarity - PFAM Model Name: PF00384
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR006656 Molybdopterin oxidoreductase Protein Sequence Similarity - InterPro Domain: IPR006656
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR006655 Molybdopterin oxidoreductase, prokaryotic, conserved site Protein Sequence Similarity - InterPro Domain: IPR006655
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF01568 Molydopterin dinucleotide binding domain Protein Sequence Similarity - PFAM Model Name: PF01568
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR006657 Molybdopterin dinucleotide-binding domain Protein Sequence Similarity - InterPro Domain: IPR006657
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR006963 Molybdopterin oxidoreductase, 4Fe-4S domain Protein Sequence Similarity - InterPro Domain: IPR006963
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR006468 Nitrate reductase, alpha subunit Protein Sequence Similarity - InterPro Domain: IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR028189 Nitrate reductase, alpha subunit, N-terminal Protein Sequence Similarity - InterPro Domain: IPR028189
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR009010 Aspartate decarboxylase-like domain superfamily Protein Sequence Similarity - InterPro Domain: IPR009010
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Automated Process Uncurated EC number 1.-.-.- Published Annotation - Database Name: EC2GO: GO:0016491
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved EC number 1.7.99.4 Published Annotation - Swiss-Prot Accession Number: P09152 Feb 2006
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.4.2 (metabolism; energy production/transport; electron acceptor) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945782 Published Annotation - Entrez Gene Database ID: 945782
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref UniProtKB/Swiss-Prot:P09152 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P09152 Published Annotation - Swiss-Prot Accession Number: P09152 Jan 2005
Automated Process Approved db xref ASAP:ABE-0004119 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1787477 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG10638 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Energy metabolism, carbon: Anaerobic respiration Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved molecular interaction Interacts with the product of dnaJ ASAP:54:(interacting residues not known); by MALDI-TOF Experimental - PubMed ID: 15690043
Reference: Escherichia coli K-12 W3110 		Mar 2005
Person Approved molecular interaction Interacts with the product of narJ ASAP:4123:(interacting residues not known); by MALDI-TOF and LC-MS Experimental - PubMed ID: 15690043
Reference: Escherichia coli K-12 W3110 		Mar 2005
Person Approved molecular interaction Interacts with the product of narY ASAP:4896:(interacting residues not known); by MALDI-TOF Experimental - PubMed ID: 15690043
Reference: Escherichia coli K-12 W3110 		Mar 2005
Person Approved molecular interaction Interacts with the product of mreB ASAP:10661:(interacting residues not known); by MALDI-TOF Experimental - PubMed ID: 15690043
Reference: Escherichia coli K-12 W3110 		Mar 2005
Automated Process Approved note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74308.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0042126 nitrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006655
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006657, IPR006656, IPR006468, IPR006963
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0009325 nitrate reductase complex GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0043546 molybdopterin cofactor binding GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006657
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0030151 molybdenum ion binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006657
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0008940 nitrate reductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006655
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO molecular function GO:0009054 (DEPRECATED) electron acceptor activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0001764 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0000060 narG CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0000078 narG CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000123 narG CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0000038 narG CDS
Ortholog
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002505 narG CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0003166 narG CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0020270 narG CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001284 CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0001173 narG CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003117 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008198 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077796 narG CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001362 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001676 CDS
Automated Process Uncurated Go view Escherichia coli E2348/69 (EPEC) BCI-0001432 narG CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005817 narG CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006548 narG CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025787 narG CDS
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0005883 narG CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006011 narG CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001461 narG CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001150 narG CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001246 narG CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001530 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001523 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001474 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001493 narZ CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001305 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001914 narG CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131681 narG CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001425 narG CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0003849 CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0062292 narG CDS
Person Approved Go view Pectobacterium carotovorum WPP14 ADT-0002836 narG CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0002054 narG CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0001109 narG CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0107699 narG CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0083387 narG CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0095729 narG CDS
Person Approved Go view Shigella boydii 227 ADV-0002044 narG CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0001456 narG CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072365 narG CDS
Person Approved Go view Shigella flexneri 301 ABT-0087565 narG CDS
Person Approved Go view Shigella sonnei 046 ADD-0002145 narG CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20270mutantTn5 InsertionRequest the StrainABE-0004119narGnarG::Tn5KAN-2 at position 966 in Plus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1274864 to 1288607
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004095ychOCDSforward12737841275178Upstream
ABE-0004097narLCDScomplement12751791275829Upstream
ABE-0004099narXCDScomplement12758221277618Upstream
ABE-0004111narKCDSforward12779571279348Upstream
ABE-0285847narSncRNAforward12793371279520Upstream
ABE-0004119narGCDSforward12798641283607Matches
ABE-0004121narHCDSforward12836041285142Overlaps Downstream
ABE-0004123narJCDSforward12851391285849Downstream
ABE-0004125narICDSforward12858491286526Downstream
ABE-0285436repeat_regionforward12865941286665Downstream
ABE-0047246rttRncRNAcomplement12870661287236Downstream
ABE-0004131tprCDScomplement12870871287176Downstream
ABE-0004134tyrVtRNAcomplement12872441287328Downstream
ABE-0004136tyrTtRNAcomplement12875381287622Downstream
ABE-0004138purUCDScomplement12877821288624Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.