Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004121 (narH) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004121 showshow history
Names
narH JW1216 ECK1219 chlC b1225
Product
nitrate reductase A subunit beta
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1283604 1285142 1539 513
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001450 (IPR001450 (DEPRECATED) 4Fe-4S binding domain) Apr 2008
Automated Process Approved IPR006547 (IPR006547 Nitrate reductase, beta subunit) Apr 2008
Automated Process Approved IPR017896 (IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain) Jul 2018
Automated Process Approved IPR029263 (IPR029263 Respiratory nitrate reductase beta, C-terminal) Jul 2018
Automated Process Approved PF13247 (PF13247 4Fe-4S dicluster domain) Jul 2018
Automated Process Approved PF14711 (PF14711 Respiratory nitrate reductase beta C-terminal) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006547
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0042126 nitrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006547
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0009325 nitrate reductase complex GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006547
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0008940 nitrate reductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006547
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0009055 electron transfer activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO molecular function GO:0009054 (DEPRECATED) electron acceptor activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Uncurated name narH Published Annotation - PubMed ID: 6298188
Reference: Escherichia coli K-12 		Jan 2007
Person Approved synonym ECK1219 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym chlC Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved synonym JW1216 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1225 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product nitrate reductase A subunit beta Published Annotation - EcoCyc Accession Number: EG10639
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Uncurated alternate product name nitrate reductase 1, Fe-S (beta) subunit Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name nitrate reductase 1, beta subunit Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Approved alternate product name nitrate reductase 1, beta (Fe-S) subunit Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family PF13247 4Fe-4S dicluster domain Protein Sequence Similarity - PFAM Model Name: PF13247
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF14711 Respiratory nitrate reductase beta C-terminal Protein Sequence Similarity - PFAM Model Name: PF14711
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR029263 Respiratory nitrate reductase beta, C-terminal Protein Sequence Similarity - InterPro Domain: IPR029263
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain Protein Sequence Similarity - InterPro Domain: IPR017896
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR006547 Nitrate reductase, beta subunit Protein Sequence Similarity - InterPro Domain: IPR006547
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR001450 (DEPRECATED) 4Fe-4S binding domain Protein Sequence Similarity - InterPro Domain: IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Approved EC number 1.7.99.4 Published Annotation - Swiss-Prot Accession Number: P11349 Feb 2006
Person Uncurated MultiFun 1.4.2 (metabolism; energy production/transport; electron acceptor) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG10639 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P11349 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P11349 Published Annotation - Swiss-Prot Accession Number: P11349 Jan 2005
Automated Process Approved db xref GeneID:945780 Published Annotation - Entrez Gene Database ID: 945780
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref ASAP:ABE-0004121 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1787478 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Energy metabolism, carbon: Anaerobic respiration Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Uncurated molecular interaction Interacts with the product of narG ASAP:4119:(interacting residues not known); by MALDI-TOF and LC-MS Experimental - PubMed ID: 15690043
Reference: Escherichia coli K-12 W3110 		Mar 2005
Automated Process Approved note nitrate reductase 1, beta subunit; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74309.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.97
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006547
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0042126 nitrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006547
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0009325 nitrate reductase complex GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006547
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0008940 nitrate reductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006547
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0009055 electron transfer activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO molecular function GO:0009054 (DEPRECATED) electron acceptor activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0001765 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0000056 narH CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0000077 narH CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000104 narH CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0000040 narH CDS
Ortholog
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002504 narH CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0003165 narH CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0020271 narH CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001285 CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0001174 narH CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003116 narH CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008197 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077797 narH CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001363 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001677 CDS
Automated Process Uncurated Go view Escherichia coli E2348/69 (EPEC) BCI-0001433 narH CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005818 narH CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006549 narH CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025788 narH CDS
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0005884 narH CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006012 narH CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001460 narH CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001151 narH CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001247 narH CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001529 narH CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001524 narH CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001475 narH CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001494 narH CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175571 narH CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001306 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001913 narH CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131682 narH CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001426 narH CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0003848 narH CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0062293 narH CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0002049 narH CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0001110 narH CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0107705 narH CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0083386 narH CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0095726 narH CDS
Person Approved Go view Shigella boydii 227 ADV-0002043 narH CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0001457 narH CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072366 narH CDS
Person Approved Go view Shigella flexneri 301 ABT-0087566 narH CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001317 narH CDS
Person Approved Go view Shigella sonnei 046 ADD-0002144 narH CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1278604 to 1290142
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004111narKCDSforward12779571279348Upstream
ABE-0285847narSncRNAforward12793371279520Upstream
ABE-0004119narGCDSforward12798641283607Overlaps Upstream
ABE-0004121narHCDSforward12836041285142Matches
ABE-0004123narJCDSforward12851391285849Overlaps Downstream
ABE-0004125narICDSforward12858491286526Downstream
ABE-0285436repeat_regionforward12865941286665Downstream
ABE-0047246rttRncRNAcomplement12870661287236Downstream
ABE-0004131tprCDScomplement12870871287176Downstream
ABE-0004134tyrVtRNAcomplement12872441287328Downstream
ABE-0004136tyrTtRNAcomplement12875381287622Downstream
ABE-0004138purUCDScomplement12877821288624Downstream
ABE-0004140ychJCDScomplement12886741289132Downstream
ABE-0004144rssACDSforward12892451290150Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.