Escherichia coli K-12 MG1655
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004123 (narJ) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004123 showshow history
Names
narJ JW1217 ECK1220 b1226
Product
nitrate reductase 1 molybdenum cofactor assembly chaperone
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1285139 1285849 711 237
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR003765 (IPR003765 Nitrate reductase chaperone, NarJ) Apr 2008
Automated Process Approved IPR020945 (IPR020945 DMSO/Nitrate reductase chaperone) Jul 2018
Automated Process Approved IPR036411 (IPR036411 TorD-like superfamily) Jul 2018
Automated Process Approved PF02613 (PF02613 Nitrate reductase delta subunit) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003765
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO biological process GO:0051131 chaperone-mediated protein complex assembly GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003765
Reference: Escherichia coli K-12 MG1655
Jul 2018
Person Uncurated GO biological process GO:0006457 protein folding Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0009325 nitrate reductase complex GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003765
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Uncurated GO molecular function GO:0051082 unfolded protein binding GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003765
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO molecular function GO:0008940 nitrate reductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003765
Reference: Escherichia coli K-12 MG1655
Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name narJ Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Person Approved synonym ECK1220 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW1217 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1226 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product nitrate reductase 1 molybdenum cofactor assembly chaperone Published Annotation - EcoCyc Accession Number: EG10641
Reference: Escherichia coli K-12 MG1655
Oct 2021
Person Approved alternate product name nitrate reductase 1, delta subunit, assembly function Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Jan 2006
Person Approved alternate product name molybdenum-cofactor-assembly chaperone subunit (delta subunit) of nitrate reductase 1 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name nitrate reductase 1, delta subunit, chaperone required for molybdenum cofactor assembly Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family PF02613 Nitrate reductase delta subunit Protein Sequence Similarity - PFAM Model Name: PF02613
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR036411 TorD-like superfamily Protein Sequence Similarity - InterPro Domain: IPR036411
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR020945 DMSO/Nitrate reductase chaperone Protein Sequence Similarity - InterPro Domain: IPR020945
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR003765 Nitrate reductase chaperone, NarJ Protein Sequence Similarity - InterPro Domain: IPR003765
Reference: Escherichia coli K-12 MG1655
Apr 2008
Other
Person Approved EC number 1.7.99.4 Published Annotation - Swiss-Prot Accession Number: P0AF26 Feb 2006
Person Uncurated MultiFun 2.3.4 (information transfer; protein related; chaperoning, folding) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0AF26 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GeneID:945807 Published Annotation - Entrez Gene Database ID: 945807
Reference: Escherichia coli K-12 MG1655
Jun 2009
Person Approved db xref UniProtKB:P0AF26 Published Annotation - Swiss-Prot Accession Number: P0AF26 Jan 2006
Automated Process Approved db xref EcoGene:EG10641 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref ASAP:ABE-0004123 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GI:1787479 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved function enzyme; Energy metabolism, carbon: Anaerobic respiration Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Person Uncurated molecular interaction Interacts with the product of narH ASAP:4121:(interacting residues not known); by MALDI-TOF and LC-MS Experimental - PubMed ID: 15690043
Reference: Escherichia coli K-12 W3110
Mar 2005
Person Uncurated molecular interaction Interacts with the product of narG ASAP:4119:(interacting residues not known); by MALDI-TOF and LC-MS Experimental - PubMed ID: 15690043
Reference: Escherichia coli K-12 W3110
Mar 2005
Automated Process Approved note nitrate reductase 1, delta subunit, assembly function; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0006457 - protein folding Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved protein id AAC74310.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655
Feb 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655
Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655
Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0006457 protein folding Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0051131 chaperone-mediated protein complex assembly GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003765
Reference: Escherichia coli K-12 MG1655
Jul 2018
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003765
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Approved GO cellular component GO:0009325 nitrate reductase complex GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003765
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO molecular function GO:0051082 unfolded protein binding GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003765
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO molecular function GO:0008940 nitrate reductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003765
Reference: Escherichia coli K-12 MG1655
Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002504 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002465 narJ CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002355 narJ CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002001 narJ CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002296 narJ CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0001865 narJ CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001673 narJ CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002503 narJ CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0003164 narJ CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0006605 CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0020272 narJ CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0002257 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0003076 narJ CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003845 narJ CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006093 narJ CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001286 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009821 narJ CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001175 narJ CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003115 narJ CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008196 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005128 narJ CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0006857 narJ pseudogene
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077798 narJ CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001364 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001678 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008516 narJ CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008599 narJ CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001434 narJ CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005819 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001427 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0010423 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009457 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0009115 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0008881 narJ CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006550 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0009449 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0010343 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0010043 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0010529 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009910 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0009232 narJ CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008028 narJ CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025789 narJ CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0005885 narJ CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006013 narJ CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001459 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001152 narJ CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001248 narJ CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001528 narJ CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001525 narJ CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001476 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001495 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175572 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001307 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001912 narJ CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131683 narJ CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004341 CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001427 narJ CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0001562 narJ CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0003847 narJ CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0002218 narJ CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0000325 narJ CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0006478 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0002338 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0002560 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0000793 CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0006170 narJ CDS
Person Approved Go view Pectobacterium atrosepticum SCRI1043 ABL-0062294 narJ CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0001054 narJ CDS
Person Approved Go view Pectobacterium carotovorum WPP14 ADT-0000528 narJ CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0003626 narJ CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0007190 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0005554 narJ CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0001239 CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0002048 narJ CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0005833 narJ CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0004178 narJ CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0004131 narJ CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0004694 narJ CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006319 narJ CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0005259 narJ CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0005313 narJ CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0008080 narJ CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0005394 narJ CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0006258 narJ CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0004525 narJ CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0001113 CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0001111 narJ CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0001481 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006507 narJ CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0008942 narJ CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0005982 narJ CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007059 narJ CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0107710 narJ CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0083385 narJ CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0002132 narJ CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0095723 narJ CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007571 narJ CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0006824 narJ CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0007373 narJ CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0002897 narJ CDS
Person Approved Go view Shigella boydii 227 ADV-0002042 narJ CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006797 narJ CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0007028 narJ CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0001458 narJ CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072367 narJ CDS
Person Approved Go view Shigella flexneri 301 ABT-0087567 narJ CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001318 narJ CDS
Person Approved Go view Shigella sonnei 046 ADD-0002143 narJ CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0000339 narJ CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0014665 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003558 narJ CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)

Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1280139 to 1290849
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004119narGCDSforward12798641283607Upstream
ABE-0004121narHCDSforward12836041285142Overlaps Upstream
ABE-0004123narJCDSforward12851391285849Matches
ABE-0004125narICDSforward12858491286526Overlaps Downstream
ABE-0285436repeat_regionforward12865941286665Downstream
ABE-0047246rttRncRNAcomplement12870661287236Downstream
ABE-0004131tprCDScomplement12870871287176Downstream
ABE-0004134tyrVtRNAcomplement12872441287328Downstream
ABE-0004136tyrTtRNAcomplement12875381287622Downstream
ABE-0004138purUCDScomplement12877821288624Downstream
ABE-0004140ychJCDScomplement12886741289132Downstream
ABE-0004144rssACDSforward12892451290150Downstream
ABE-0004147rssBCDSforward12902421291255Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.