Escherichia coli K-12 MG1655
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004125 (narI) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004125 showshow history
Names
narI JW1218 ECK1221 chlI b1227
Product
nitrate reductase A subunit gamma
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1285849 1286526 678 226
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR003816 (IPR003816 Nitrate reductase, gamma subunit) Apr 2008
Automated Process Approved IPR023234 (IPR023234 NarG-like domain) Jul 2018
Automated Process Approved IPR036197 (IPR036197 NarG-like superfamily) Jul 2018
Automated Process Approved PF02665 (PF02665 Nitrate reductase gamma subunit) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003816
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003816
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO biological process GO:0017004 cytochrome complex assembly Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0009325 nitrate reductase complex GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003816
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Approved GO molecular function GO:0008940 nitrate reductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003816
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO molecular function GO:0009054 (DEPRECATED) electron acceptor activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name narI Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Person Approved synonym JW1218 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym chlI Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved synonym ECK1221 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1227 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product nitrate reductase A subunit gamma Published Annotation - EcoCyc Accession Number: EG10640
Reference: Escherichia coli K-12 MG1655
Oct 2021
Person Approved alternate product name nitrate reductase 1, cytochrome b(NR), gamma subunit Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Jan 2006
Person Approved alternate product name nitrate reductase 1, gamma (cytochrome b(NR)) subunit Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family IPR036197 NarG-like superfamily Protein Sequence Similarity - InterPro Domain: IPR036197
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR023234 NarG-like domain Protein Sequence Similarity - InterPro Domain: IPR023234
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family PF02665 Nitrate reductase gamma subunit Protein Sequence Similarity - PFAM Model Name: PF02665
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR003816 Nitrate reductase, gamma subunit Protein Sequence Similarity - InterPro Domain: IPR003816
Reference: Escherichia coli K-12 MG1655
Apr 2008
Other
Person Approved EC number 1.7.99.4 Published Annotation - Swiss-Prot Accession Number: P11350 Feb 2006
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.4.2 (metabolism; energy production/transport; electron acceptor) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.6.15.1 (metabolism; macromolecules (cellular constituent) biosynthesis; large molecule carriers; cytochromes) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref EcoGene:EG10640 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GeneID:945808 Published Annotation - Entrez Gene Database ID: 945808
Reference: Escherichia coli K-12 MG1655
Jun 2009
Person Approved db xref UniProtKB:P11350 Published Annotation - Swiss-Prot Accession Number: P11350 Jan 2005
Automated Process Approved db xref GI:1787480 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P11350 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref ASAP:ABE-0004125 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved function enzyme; Energy metabolism, carbon: Anaerobic respiration Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Automated Process Approved note nitrate reductase 1, cytochrome b(NR), gamma subunit; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0017004 - cytochrome complex assembly Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved protein id AAC74311.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655
Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655
Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655
Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003816
Reference: Escherichia coli K-12 MG1655
Jul 2018
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO biological process GO:0017004 cytochrome complex assembly Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003816
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0009325 nitrate reductase complex GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003816
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated GO molecular function GO:0009054 (DEPRECATED) electron acceptor activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0008940 nitrate reductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003816
Reference: Escherichia coli K-12 MG1655
Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002505 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002443 narI CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002362 narI CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002000 narI CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002273 narI CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0001866 narI CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001674 narI CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002502 narI CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0003163 narI CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0006606 CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0020273 narI CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0002256 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0003075 narI CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003846 narI2 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006094 narI CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001287 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009822 narI CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001176 narI CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003114 narI CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008195 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005129 narI CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0006856 pseudogene
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077799 narI CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001365 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001679 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008515 narI CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008600 narI CDS
Automated Process Uncurated Go view Escherichia coli E2348/69 (EPEC) BCI-0001435 narI CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005820 narI1 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001428 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0010424 narI CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009458 narI CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0009116 narI CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0008882 narI CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006551 narI CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0009448 narI CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0010344 narI CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0010044 narI CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0010530 narI CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009909 narI CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0009233 narI CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008029 narI CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025790 narI CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0005886 narI CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006014 narI2 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001458 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001153 narI CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001249 narI CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001527 narI CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001526 narI CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001477 narI CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175573 narI CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001308 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001911 narI CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131684 narI CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004340 CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001428 narI CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0001563 narI CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0003846 narI CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0002217 narI CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0000324 narI CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0006479 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0002337 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0002559 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0000792 CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0006169 narI CDS
Person Approved Go view Pectobacterium atrosepticum SCRI1043 ABL-0062295 narI CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0003936 CDS
Person Approved Go view Pectobacterium carotovorum WPP14 ADT-0002838 narI CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0003627 narI CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0007189 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0005555 narI CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0001240 CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0002047 narI CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0005834 narI CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0004180 narI CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0004133 narI CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0004695 narI CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006318 narI CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0005258 narI CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0005312 narI CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0008079 narI CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0005393 narI CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0006257 narI CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0004526 narI CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0001114 CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0001112 narI CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0001482 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006506 narI CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0008943 narI CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0005981 narI CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007058 narI CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0107711 narI CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0083384 narI CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0002131 narI CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0095721 narI CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007572 narI CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0006823 narI CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0007372 narI CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0002898 narI CDS
Person Approved Go view Shigella boydii 227 ADV-0002041 narI CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006798 narI CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0007027 narI CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0001459 narI CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072368 narI CDS
Person Approved Go view Shigella flexneri 301 ABT-0087568 narI CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001319 narI CDS
Person Approved Go view Shigella sonnei 046 ADD-0002142 narI CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0000338 narI CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0014663 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003559 narI CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)

Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1280849 to 1291526
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004119narGCDSforward12798641283607Upstream
ABE-0004121narHCDSforward12836041285142Upstream
ABE-0004123narJCDSforward12851391285849Overlaps Upstream
ABE-0004125narICDSforward12858491286526Matches
ABE-0285436repeat_regionforward12865941286665Downstream
ABE-0047246rttRncRNAcomplement12870661287236Downstream
ABE-0004131tprCDScomplement12870871287176Downstream
ABE-0004134tyrVtRNAcomplement12872441287328Downstream
ABE-0004136tyrTtRNAcomplement12875381287622Downstream
ABE-0004138purUCDScomplement12877821288624Downstream
ABE-0004140ychJCDScomplement12886741289132Downstream
ABE-0004144rssACDSforward12892451290150Downstream
ABE-0004147rssBCDSforward12902421291255Downstream
ABE-0004150galUCDSforward12914571292365Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.