Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004189 (kch) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004189 showshow history
Names
kch JW1242 ECK1244 b1250
Product
voltage-gated potassium channel protein
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1309016 1310269 1254 418
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR003148 (IPR003148 Regulator of K+ conductance, N-terminal) Apr 2008
Automated Process Approved IPR013099 (IPR013099 Potassium channel domain) Apr 2008
Automated Process Approved IPR036291 (IPR036291 NAD(P)-binding domain superfamily) Jul 2018
Automated Process Approved PF07885 (PF07885 Ion channel) Jul 2018
Automated Process Approved PF02254 (PF02254 TrkA-N domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006813 potassium ion transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003148
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Approved GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Approved GO molecular function GO:0005244 voltage-gated ion channel activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name kch Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK1244 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW1242 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1250 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product voltage-gated potassium channel protein Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 		Oct 2021
Person Approved alternate product name putative potassium channel protein (VIC family) Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR036291 NAD(P)-binding domain superfamily Protein Sequence Similarity - InterPro Domain: IPR036291
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR013099 Potassium channel domain Protein Sequence Similarity - InterPro Domain: IPR013099
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR003148 Regulator of K+ conductance, N-terminal Protein Sequence Similarity - InterPro Domain: IPR003148
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF07885 Ion channel Protein Sequence Similarity - PFAM Model Name: PF07885
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF02254 TrkA-N domain Protein Sequence Similarity - PFAM Model Name: PF02254
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved MultiFun 4.1.A.1 (transport; Channel-type Transporters; alpha-type channels; The Voltage-gated Ion Channel (VIC) Superfamily) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Approved MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Approved MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Approved MultiFun 4.S.96 (transport; substrate; K+) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945841 Published Annotation - Entrez Gene Database ID: 945841
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P31069 Published Annotation - Swiss-Prot Accession Number: P31069 Jan 2005
Automated Process Approved db xref UniProtKB/Swiss-Prot:P31069 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0004189 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1787503 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11606 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved function 4.1.A.1 transport; Channel-type Transporters; alpha-type channels; The Voltage-gated Ion Channel (VIC) Superfamily Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved function 4.S.96 transport; substrate; K+ Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved function 6.1 cell structure; membrane Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved function 7.3 location of gene products; inner membrane Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved note putative potassium channel protein (VIC family); GO_component: GO:0019866 - organelle inner membrane; GO_function: GO:0005244 - voltage-gated ion channel activity Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74332.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006813 potassium ion transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003148
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Approved GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Approved GO molecular function GO:0005244 voltage-gated ion channel activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0006707 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003152 kch CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003152 kch CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0007665 kch CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003221 kch CDS
Ortholog
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003880 kch CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006118 kch CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001310 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009848 kch CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001197 kch CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0003093 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008168 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005152 kch CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0006831 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0077829 kch CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001388 kch CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001703 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008491 kch CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008621 kch CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001464 kch CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005840 kch CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001447 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0010449 kch CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009483 kch CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0010686 kch CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006577 kch CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0009424 kch CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0010368 kch CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0010066 kch CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0011086 kch CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0010598 kch CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0009256 kch CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008055 kch CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025810 kch CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0005911 kch CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006035 kch CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001827 CDS
Automated Process Uncurated Go view Escherichia coli K-12 DH10B AEC-0004451 kch CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001276 kch CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001508 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001606 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001497 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001525 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175596 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001328 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001889 kch CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131704 kch CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004321 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001453 kch CDS
Person Approved Go view Shigella boydii 227 ADV-0002018 kch CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006823 kch CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0007005 kch CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0001492 kch CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072393 kch CDS
Person Approved Go view Shigella flexneri 301 ABT-0087590 kch CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001339 kch CDS
Person Approved Go view Shigella sonnei 046 ADD-0002122 kch CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0002042 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0002892 CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0003040 kch CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0001774 kch CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0002094 CDS
Automated Process Uncurated Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0240282 kch CDS
Automated Process Uncurated Go view Yersinia pestis Angola (biovar Pestoides) ADI-0007686 kch CDS
Automated Process Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0002891 CDS
Automated Process Uncurated Go view Yersinia pestis B42003004 (biovar Antiqua) BAL-0006479 kch CDS
Automated Process Uncurated Go view Yersinia pestis CA88-4125 (biovar Orientalis) BAF-0000807 CDS
Automated Process Uncurated Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0124236 CDS
Automated Process Uncurated Go view Yersinia pestis E1979001 (biovar Antiqua) BAM-0004466 kch CDS
Automated Process Uncurated Go view Yersinia pestis F1991016 (biovar Orientalis) BAQ-0005368 kch CDS
Automated Process Uncurated Go view Yersinia pestis FV-1 (biovar Orientalis) BAK-0002030 CDS
Automated Process Uncurated Go view Yersinia pestis IP275 (biovar Orientalis) BAC-0009371 kch CDS
Automated Process Uncurated Go view Yersinia pestis K1973002 (biovar Mediaevalis) BAO-0004743 kch CDS
Person Uncurated Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0030917 CDS
Automated Process Uncurated Go view Yersinia pestis MG05-1020 (biovar Orientalis) BAP-0007246 kch CDS
Automated Process Uncurated Go view Yersinia pestis Nepal516 (biovar Antiqua) BAD-0001406 CDS
Automated Process Uncurated Go view Yersinia pestis Pestoides F (biovar Pestoides) BAI-0001909 CDS
Automated Process Uncurated Go view Yersinia pestis UG05-0454 (biovar Antiqua) BAN-0005301 kch CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0005043 kch CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0003030 kch CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0003642 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0001338 CDS
Molecular Interactions to toptop
Author Approval Feature Name Technique Evidence Comment Annotated
Person Approved ABE-0004189 kch lambda repressor assay Doodle - bnumber: b1250 Oct 2003
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22802mutantTn5 InsertionRequest the StrainABE-0004189kchkch::Tn5(KAN-2) at position 124 in Minus orientation ,- pKD46 0Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1304016 to 1315269
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004176oppCCDSforward13038341304742Downstream
ABE-0004178oppDCDSforward13047541305767Downstream
ABE-0004182oppFCDSforward13057641306768Downstream
ABE-0004184yciUCDScomplement13068211307150Downstream
ABE-0004186clsACDScomplement13071851308645Downstream
ABE-0285179yciYCDSforward13087881308961Downstream
ABE-0004189kchCDScomplement13090161310269Matches
ABE-0004192yciICDScomplement13105691310865Upstream
ABE-0004196tonBCDSforward13110891311808Upstream
ABE-0004198yciACDScomplement13118481312246Upstream
ABE-0004200yciBCDScomplement13123511312890Upstream
ABE-0004204yciCCDScomplement13129201313663Upstream
ABE-0004210ompWCDSforward13140201314658Upstream
ABE-0004212yciECDScomplement13147181315224Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.