Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004362 (puuP) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004362 showshow history
Names
puuP ycjJ JW1289 ECK1291 b1296
Product
putrescine importer
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1357802 1359187 1386 462
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR002293 (IPR002293 Amino acid/polyamine transporter I) Apr 2008
Automated Process Approved IPR004841 (IPR004841 Amino acid permease/ SLC12A domain) Apr 2008
Automated Process Approved PF00324 (PF00324 Amino acid permease) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006865 amino acid transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002293
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004841
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004841
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002293
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0015171 amino acid transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002293
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name puuP Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW1289 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ycjJ Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Approved synonym ECK1291 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1296 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product putrescine importer Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name putative transport protein (APC family) Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name putative amino acid/amine transport protein Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR004841 Amino acid permease/ SLC12A domain Protein Sequence Similarity - InterPro Domain: IPR004841
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00324 Amino acid permease Protein Sequence Similarity - PFAM Model Name: PF00324
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR002293 Amino acid/polyamine transporter I Protein Sequence Similarity - InterPro Domain: IPR002293
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 4.2.A.3 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Amino Acid-Polyamine-Choline (APC) Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG13907 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0004362 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P76037 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P76037 Published Annotation - Swiss-Prot Accession Number: P76037 Jan 2005
Automated Process Approved db xref GeneID:946287 Published Annotation - Entrez Gene Database ID: 946287
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:87081869 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function putative transport; Not classified Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note putative amino acid/amine transport protein; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74378.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004841
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0006865 amino acid transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002293
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004841
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002293
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0015171 amino acid transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002293
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Homolog
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0267724 CDS
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0000962 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0000052 puuP CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0000045 puuP CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000030 puuP CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0000050 puuP CDS
Ortholog
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0001593 puuP_1 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0002499 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0004100 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006168 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009903 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008112 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005214 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005237 CDS
Person Uncurated Go view Escherichia coli CFT073 (UPEC) ABR-0082065 puuP pseudogene
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001437 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001753 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0005444 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0006110 CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005945 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0002226 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0005317 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009682 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0010574 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007198 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006778 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007753 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0011253 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007923 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008807 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0008279 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007330 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008693 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026233 puuP CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006087 CDS
Automated Process Uncurated Go view Escherichia coli K-12 DH10B AEC-0004502 puuP CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001325 puuP CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001454 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001552 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001652 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001377 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001825 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131837 puuP CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0002265 CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0002127 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0001026 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0003723 CDS
Person Approved Go view Shigella boydii 227 ADV-0001963 ycjJ CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006938 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0006521 CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072445 ycjJ CDS
Person Approved Go view Shigella flexneri 301 ABT-0087638 ycjJ CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001388 ycjJ CDS
Person Approved Go view Shigella sonnei 046 ADD-0002025 puuP CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0016135 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003198 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22403mutantTn5 InsertionRequest the StrainABE-0004362puuPycjJ::Tn5KAN-I-SceI at position 263 in Minus orientation ,+ pKD460Blattner Laboratory
FB23075mutantTn5 InsertionRequest the StrainABE-0004362puuPycjJ::Tn5KAN-I-SceI at position 263 in Minus orientation ,- pKD465Tim Durfee (durf@genome.wisc.edu)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1352802 to 1364187
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004342sapDCDScomplement13526361353628Downstream
ABE-0004348sapCCDScomplement13536281354518Downstream
ABE-0004350sapBCDScomplement13545051355470Downstream
ABE-0004354sapACDScomplement13554671357110Downstream
ABE-0285439repeat_regionforward13573591357392Downstream
ABE-0004359ymjACDScomplement13574231357668Downstream
ABE-0285440repeat_regionforward13577701357799Downstream
ABE-0004362puuPCDScomplement13578021359187Matches
ABE-0285746ymjECDScomplement13591771359341Overlaps Upstream
ABE-0004365puuACDScomplement13594901360908Upstream
ABE-0004369puuDCDSforward13611201361884Upstream
ABE-0004371puuRCDSforward13619111362468Upstream
ABE-0004376puuCCDSforward13627431364230Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.