Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004365 (puuA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004365 showshow history
Names
puuA ycjK JW5201 ECK1292 b1297
Product
gamma-Glu-putrescine synthase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1359490 1360908 1419 473
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR014746 (IPR014746 Glutamine synthetase/guanido kinase, catalytic domain) Apr 2008
Automated Process Approved IPR008147 (IPR008147 Glutamine synthetase, beta-Grasp domain) Apr 2008
Automated Process Approved IPR008146 (IPR008146 Glutamine synthetase, catalytic domain) Apr 2008
Automated Process Approved IPR036651 (IPR036651 Glutamine synthetase, N-terminal domain superfamily) Jul 2018
Automated Process Approved PF00120 (PF00120 Glutamine synthetase, catalytic domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Approved GO biological process GO:0006542 glutamine biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006542 glutamine biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008147
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006807 nitrogen compound metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008147
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Approved GO biological process GO:0006807 nitrogen compound metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0004356 glutamate-ammonia ligase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008147
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR014746
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name puuA Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW5201 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ycjK Published Annotation - Swiss-Prot Accession Number: P78061 Jan 2006
Person Approved synonym ECK1292 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1297 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product gamma-Glu-putrescine synthase Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family IPR008147 Glutamine synthetase, beta-Grasp domain Protein Sequence Similarity - InterPro Domain: IPR008147
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR036651 Glutamine synthetase, N-terminal domain superfamily Protein Sequence Similarity - InterPro Domain: IPR036651
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00120 Glutamine synthetase, catalytic domain Protein Sequence Similarity - PFAM Model Name: PF00120
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR008146 Glutamine synthetase, catalytic domain Protein Sequence Similarity - InterPro Domain: IPR008146
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR014746 Glutamine synthetase/guanido kinase, catalytic domain Protein Sequence Similarity - InterPro Domain: IPR014746
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Automated Process Uncurated EC number 6.3.1.2 Published Annotation - Database Name: EC2GO: GO:0004356
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved EC number 6.3.1.11 Published Annotation - Swiss-Prot Accession Number: P78061 Feb 2006
Person Uncurated MultiFun 1.5.1.2 (metabolism; building block biosynthesis; amino acids; glutamine) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.8.3 (metabolism; metabolism of other compounds; nitrogen metabolism) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG13908 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0004365 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946202 Published Annotation - Entrez Gene Database ID: 946202
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:87081870 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P78061 Published Annotation - Swiss-Prot Accession Number: P78061 Jan 2005
Automated Process Approved db xref UniProtKB/Swiss-Prot:P78061 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved note putative glutamine synthetase; GO_process: GO:0006542 - glutamine biosynthetic process; GO_process: GO:0006807 - nitrogen compound metabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74379.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.97
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006807 nitrogen compound metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008147
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Approved GO biological process GO:0006542 glutamine biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Approved GO biological process GO:0006807 nitrogen compound metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006542 glutamine biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008147
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR014746
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0004356 glutamate-ammonia ligase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR008147
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003548 puuA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002958 puuA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000044 puuA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003270 puuA CDS
Ortholog
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0001592 puuA CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0002498 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006169 puuA CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009904 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008111 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005215 puuA CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005236 CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001438 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001754 puuA CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0006111 puuA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005946 puuA CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0002225 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0005318 puuA CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009683 puuA CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007197 puuA CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006779 puuA CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007754 puuA CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0011254 puuA CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007924 puuA CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008808 puuA CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0008278 puuA CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007331 puuA CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008692 puuA CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026232 puuA CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006088 puuA CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001214 puuA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001326 puuA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001453 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001551 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001651 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001378 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001824 puuA CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131838 puuA CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0002264 CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0002311 CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0002124 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0001025 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0001836 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0001792 puuA CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0002245 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0002532 puuA CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0001613 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0010294 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0289484 puuA CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0005706 ycjK CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0005388 CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0264833 CDS
Person Approved Go view Shigella boydii 227 ADV-0001962 CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006939 puuA CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0006520 puuA CDS
Person Uncurated Go view Shigella dysenteriae 197 ADU-0002247 CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072446 CDS
Person Approved Go view Shigella flexneri 301 ABT-0087639 CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001389 CDS
Person Approved Go view Shigella sonnei 046 ADD-0002024 puuA CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0016139 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003197 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22404mutantTn5 InsertionRequest the StrainABE-0004365puuAb1297::Tn5KAN-I-SceI at position 1358 in Minus orientation ,+ pKD460Blattner Laboratory
FB23076mutantTn5 InsertionRequest the StrainABE-0004365puuAb1297::Tn5KAN-I-SceI at position 1358 in Minus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1354490 to 1365908
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004348sapCCDScomplement13536281354518Downstream
ABE-0004350sapBCDScomplement13545051355470Downstream
ABE-0004354sapACDScomplement13554671357110Downstream
ABE-0285439repeat_regionforward13573591357392Downstream
ABE-0004359ymjACDScomplement13574231357668Downstream
ABE-0285440repeat_regionforward13577701357799Downstream
ABE-0004362puuPCDScomplement13578021359187Downstream
ABE-0285746ymjECDScomplement13591771359341Downstream
ABE-0004365puuACDScomplement13594901360908Matches
ABE-0004369puuDCDSforward13611201361884Upstream
ABE-0004371puuRCDSforward13619111362468Upstream
ABE-0004376puuCCDSforward13627431364230Upstream
ABE-0004378puuBCDSforward13642321365512Upstream
ABE-0004380puuECDSforward13655501366815Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.