Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004378 (puuB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004378 showshow history
Names
puuB ycjA ordL JW1294 ECK1296 b1301
Product
gamma-glutamylputrescine oxidase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1364232 1365512 1281 427
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001100 (IPR001100 Pyridine nucleotide-disulphide oxidoreductase, class I) Apr 2008
Automated Process Approved IPR006076 (IPR006076 FAD dependent oxidoreductase) Apr 2008
Automated Process Approved IPR036188 (IPR036188 FAD/NAD(P)-binding domain superfamily) Jul 2018
Automated Process Approved PF01266 (PF01266 FAD dependent oxidoreductase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001100
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006076
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001100
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name puuB Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK1296 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym ycjA Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym JW1294 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ordL Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Approved locus tag b1301 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product gamma-glutamylputrescine oxidase Published Annotation - EcoCyc Accession Number: EG11822
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Approved alternate product name gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name putative oxidoreductase Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name probable oxidoreductase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR001100 Pyridine nucleotide-disulphide oxidoreductase, class I Protein Sequence Similarity - InterPro Domain: IPR001100
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR006076 FAD dependent oxidoreductase Protein Sequence Similarity - InterPro Domain: IPR006076
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR036188 FAD/NAD(P)-binding domain superfamily Protein Sequence Similarity - InterPro Domain: IPR036188
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF01266 FAD dependent oxidoreductase Protein Sequence Similarity - PFAM Model Name: PF01266
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 1.4.3.- Published Annotation - Swiss-Prot Accession Number: P37906 Feb 2006
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0004378 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P37906 Published Annotation - Swiss-Prot Accession Number: P37906 Jan 2005
Automated Process Approved db xref GeneID:945072 Published Annotation - Entrez Gene Database ID: 945072
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:1787559 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P37906 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11822 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function putative enzyme; Not classified Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note probable oxidoreductase Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74383.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006076
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001100
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001100
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002340 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002441 puuB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002527 puuB CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000071 puuB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002757 puuB CDS
Ortholog
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0001588 puuB CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0002494 puuB CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0004095 puuB CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006173 puuB CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009908 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008107 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005219 puuB CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005232 CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001445 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001758 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0005437 puuB CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0006115 puuB CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0005951 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000940 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0005322 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009687 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0009351 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007193 puuB CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006783 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007758 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0011258 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007928 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008812 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0008274 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007335 puuB CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008688 puuB CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026228 puuB CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006092 puuB CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001218 puuB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001330 puuB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001449 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001547 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001646 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001382 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001820 puuB CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0131842 puuB CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0002260 CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0002120 puuB CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0001021 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0001839 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0001956 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0001795 puuB CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0002242 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0002535 puuB CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0001610 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0010297 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0001342 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0289487 puuB CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0005709 ordl3 CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0002739 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0003728 puuB CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0264839 CDS
Person Uncurated Go view Shigella boydii 227 ADV-0001958 ordL CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006943 puuB CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0006515 puuB CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0002243 ordL CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072450 ordL CDS
Person Approved Go view Shigella flexneri 301 ABT-0087643 ordL CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001393 ordL CDS
Person Approved Go view Shigella sonnei 046 ADD-0002020 puuB CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0016158 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003193 puuB CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20287mutantTn5 InsertionRequest the StrainABE-0004378puuBordL::Tn5KAN-2 at position 291 in Plus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1359232 to 1370512
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0285746ymjECDScomplement13591771359341Upstream
ABE-0004365puuACDScomplement13594901360908Upstream
ABE-0004369puuDCDSforward13611201361884Upstream
ABE-0004371puuRCDSforward13619111362468Upstream
ABE-0004376puuCCDSforward13627431364230Upstream
ABE-0004378puuBCDSforward13642321365512Matches
ABE-0004380puuECDSforward13655501366815Downstream
ABE-0285441repeat_regionforward13668331366913Downstream
ABE-0004383pspFCDScomplement13669351367912Downstream
ABE-0004387pspACDSforward13680791368747Downstream
ABE-0004389pspBCDSforward13688011369025Downstream
ABE-0004391pspCCDSforward13690251369384Downstream
ABE-0004394pspDCDSforward13693931369614Downstream
ABE-0004397pspECDSforward13696891370003Downstream
ABE-0004400ycjMCDSforward13702161371895Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.