Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004567 (trkG) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004567 showshow history
Names
trkG JW1358 ECK1362 b1363
Product
Rac prophage; potassium transporter subunit
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1423782 1425239 1458 486
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR003445 (IPR003445 Cation transporter) Apr 2008
Automated Process Approved IPR004772 (IPR004772 TrkH potassium transport family) Apr 2008
Automated Process Approved IPR011014 (IPR011014 Mechanosensitive ion channel MscS, transmembrane-2) Apr 2008
Automated Process Approved PF02386 (PF02386 Cation transport protein) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006812 cation transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003445
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0071805 potassium ion transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004772
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003445
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004772
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0015379 potassium:chloride symporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004772
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0008324 cation transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003445
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name trkG Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK1362 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW1358 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1363 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product Rac prophage; potassium transporter subunit Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name trk system potassium uptake protein Experimental - PubMed ID: 7896723
Reference: Escherichia coli 		Jan 2006
Person Uncurated alternate product name Rac prophage; Trk system potassium uptake Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR003445 Cation transporter Protein Sequence Similarity - InterPro Domain: IPR003445
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR011014 Mechanosensitive ion channel MscS, transmembrane-2 Protein Sequence Similarity - InterPro Domain: IPR011014
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF02386 Cation transport protein Protein Sequence Similarity - PFAM Model Name: PF02386
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR004772 TrkH potassium transport family Protein Sequence Similarity - InterPro Domain: IPR004772
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 4.2.A.38 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The K+ Transporter (Trk) Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.96 (transport; substrate; K+) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 8.1 (extrachromosomal; prophage genes and phage related functions) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11020 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P23849 Published Annotation - Swiss-Prot Accession Number: P23849 Jan 2005
Automated Process Approved db xref GeneID:945932 Published Annotation - Entrez Gene Database ID: 945932
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:1787626 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P23849 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0004567 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved function transport; Transport of small molecules: Cations; Phage or Prophage Related Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74445.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006812 cation transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003445
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0071805 potassium ion transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004772
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003445
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004772
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0015379 potassium:chloride symporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004772
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0008324 cation transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003445
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0006438 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0007212 trkG CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0006185 trkG CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004002 trkG CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0006007 trkG CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0001168 trkG CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0008724 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0004673 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0004416 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005394 CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0001352 trkG CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0006194 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001278 trkG CDS
Person Uncurated Go view Escherichia coli K-12 W3110 ADY-0001397 trkG CDS
Automated Process Uncurated Go view Escherichia coli SE11 AFS-0001460 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0007347 CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0002332 trkG CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20301mutantTn5 InsertionRequest the StrainABE-0004567trkGtrkG::Tn5KAN-2 at position 62 in Plus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1418782 to 1430239
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0285446misc_featureforward14118991434958Contains
ABE-0234208Racprophageforward14119251434984Contains
ABE-0004540sieBCDSforward14186711419159Upstream
ABE-0285048ydaFCDScomplement14191561419311Upstream
ABE-0004544ydaGCDScomplement14193221419456Upstream
ABE-0004548racRCDScomplement14197651420241Upstream
ABE-0004552ydaSCDSforward14203651420661Upstream
ABE-0004556ydaTCDSforward14206841421106Upstream
ABE-0004558ydaUCDSforward14211191421976Upstream
ABE-0004560ydaVCDSforward14219831422729Upstream
ABE-0004562ydaWpseudogeneforward14227521423201Upstream
ABE-0004564rzpRpseudogeneforward14232011423644Upstream
ABE-0285049rzoRCDSforward14234001423585Upstream
ABE-0004567trkGCDSforward14237821425239Matches
ABE-0004571ynaKCDSforward14253771425640Downstream
ABE-0004574ydaYpseudogeneforward14256301426082Downstream
ABE-0004581ynaApseudogeneforward14264541427386Downstream
ABE-0285103lomRpseudogeneforward14273871427600(Interval #1) Downstream
ABE-0004583lomR_1CDSpforward14274581427613Downstream
ABE-0004584is_elementforward14275991428794Downstream
ABE-0285447mobile_elementcomplement14275991428794Downstream
ABE-0004586insH5CDScomplement14277461428726Downstream
ABE-0285103lomRpseudogeneforward14288011428984(Interval #2) Downstream
ABE-0004590lomR_2CDSpforward14288141428984Downstream
ABE-0004595stfRCDSforward14290491432411Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.