Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004666 (paaI) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004666 showshow history
Names
paaI ydbV JW1391 ECK1393 b1396
Product
phenylacetyl-CoA thioesterase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1460471 1460893 423 141
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR006683 (IPR006683 Thioesterase domain) Apr 2008
Automated Process Approved IPR003736 (IPR003736 Phenylacetic acid degradation-related domain) Apr 2008
Automated Process Approved IPR011973 (IPR011973 Phenylacetic acid degradation protein PaaD) Apr 2008
Automated Process Approved IPR029069 (IPR029069 HotDog domain superfamily) Jul 2018
Automated Process Approved PF03061 (PF03061 Thioesterase superfamily) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0016790 thiolester hydrolase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR011973
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006683
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name paaI Experimental - PubMed ID: 9748275
Reference: Escherichia coli 		Aug 2002
Person Approved synonym JW1391 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym ydbV Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym ECK1393 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1396 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product phenylacetyl-CoA thioesterase Published Annotation - EcoCyc Accession Number: G6717
Reference: Escherichia coli K-12 MG1655 		Jan 2021
Person Approved alternate product name predicted thioesterase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name putative phenylacetic acid degradation protein Published Annotation - Database Name: GenProtEC Jan 2006
Automated Process Approved alternate product name thioesterase, most active with ring-hydroxylated phenylacetyl-coenzyme A thioesters Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Uncurated alternate product name phenylacetic acid degradation protein Experimental - PubMed ID: 9748275
Reference: Escherichia coli 		Jan 2006
Protein Family
Automated Process Approved protein family IPR003736 Phenylacetic acid degradation-related domain Protein Sequence Similarity - InterPro Domain: IPR003736
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF03061 Thioesterase superfamily Protein Sequence Similarity - PFAM Model Name: PF03061
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR011973 Phenylacetic acid degradation protein PaaD Protein Sequence Similarity - InterPro Domain: IPR011973
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR029069 HotDog domain superfamily Protein Sequence Similarity - InterPro Domain: IPR029069
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR006683 Thioesterase domain Protein Sequence Similarity - InterPro Domain: IPR006683
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Automated Process Uncurated EC number 3.1.2.- Published Annotation - Database Name: EC2GO: GO:0016790
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated MultiFun 1.1.1 (metabolism; carbon utilization; carbon compounds) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P76084 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1787662 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P76084 Published Annotation - Swiss-Prot Accession Number: P76084 Jan 2005
Automated Process Approved db xref ASAP:ABE-0004666 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945265 Published Annotation - Entrez Gene Database ID: 945265
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref EcoGene:EG13743 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved function enzyme: Phenylacetic acid degradation Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved note GO_process: GO:0016052 - carbohydrate catabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74478.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006683
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0016790 thiolester hydrolase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR011973
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0005101 paaI CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0004629 paaI CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002776 paaI CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0004169 paaI CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0002128 paaI CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001815 paaD CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0002567 paaD CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009981 paaI CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0008028 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005294 paaI CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005161 PaaI CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001512 paaD CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001831 paaD CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0005344 paaI CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0006250 paaI CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006021 paaD CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006161 paaD CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001308 paaI CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001431 paaI CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001380 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001698 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001577 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001510 CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0001782 paaI CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0001481 paaI CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0000171 paaI CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0002699 paaI CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0007077 paaD CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0008269 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0013120 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003961 paaI CDS
Molecular Interactions to toptop
Author Approval Feature Name Technique Evidence Comment Annotated
Person Approved ABE-0004666 paaI lambda repressor assay Doodle - bnumber: b1396 Oct 2003
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1455471 to 1465893
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004653paaCCDSforward14551641455910Upstream
ABE-0004655paaDCDSforward14559251456422Upstream
ABE-0004657paaECDSforward14564301457500Upstream
ABE-0004659paaFCDSforward14574971458264Upstream
ABE-0004661paaGCDSforward14582641459052Upstream
ABE-0004663paaHCDSforward14590541460481Overlaps Upstream
ABE-0285720matS8protein_bindforward14599851459997Upstream
ABE-0004666paaICDSforward14604711460893Matches
ABE-0004668paaJCDSforward14608931462098Overlaps Downstream
ABE-0004671paaKCDSforward14621251463438Downstream
ABE-0004674paaXCDSforward14635391464489Downstream
ABE-0004676paaYCDSforward14644711465061Downstream
ABE-0285217ynbGCDScomplement14651651465230Downstream
ABE-0004680ydbA_1CDSpforward14653921467950Downstream
ABE-0285093ydbApseudogeneforward14653921467909(Interval #1) Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.