Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004898 (narZ) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004898 showshow history
Names
narZ chlZ JW1463 ECK1462 b1468
Product
nitrate reductase Z subunit alpha
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1538850 1542590 3741 1247
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR006656 (IPR006656 Molybdopterin oxidoreductase) Apr 2008
Automated Process Approved IPR006657 (IPR006657 Molybdopterin dinucleotide-binding domain) Apr 2008
Automated Process Approved IPR006655 (IPR006655 Molybdopterin oxidoreductase, prokaryotic, conserved site) Apr 2008
Automated Process Approved IPR009010 (IPR009010 Aspartate decarboxylase-like domain superfamily) Apr 2008
Automated Process Approved IPR006468 (IPR006468 Nitrate reductase, alpha subunit) Apr 2008
Automated Process Approved IPR028189 (IPR028189 Nitrate reductase, alpha subunit, N-terminal) Jul 2018
Automated Process Approved IPR006963 (IPR006963 Molybdopterin oxidoreductase, 4Fe-4S domain) Jul 2018
Automated Process Approved PF00384 (PF00384 Molybdopterin oxidoreductase) Jul 2018
Automated Process Approved PF01568 (PF01568 Molydopterin dinucleotide binding domain) Jul 2018
Automated Process Approved PF14710 (PF14710 Respiratory nitrate reductase alpha N-terminal) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006656, IPR006657, IPR006468, IPR006963
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0042126 nitrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006655
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0009325 nitrate reductase complex GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0043546 molybdopterin cofactor binding GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006657
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0008940 nitrate reductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006656
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO molecular function GO:0009054 (DEPRECATED) electron acceptor activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0030151 molybdenum ion binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006657
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name narZ Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW1463 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK1462 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym chlZ Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved locus tag b1468 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product nitrate reductase Z subunit alpha Published Annotation - EcoCyc Accession Number: EG10648
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Approved alternate product name nitrate reductase 2 (NRZ), alpha subunit Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name cryptic nitrate reductase 2 alpha subunit Published Annotation - Swiss-Prot Accession Number: P19319 Jan 2006
Person Uncurated alternate product name nitrate reductase 2, alpha subunit Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR006656 Molybdopterin oxidoreductase Protein Sequence Similarity - InterPro Domain: IPR006656
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR006657 Molybdopterin dinucleotide-binding domain Protein Sequence Similarity - InterPro Domain: IPR006657
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00384 Molybdopterin oxidoreductase Protein Sequence Similarity - PFAM Model Name: PF00384
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF01568 Molydopterin dinucleotide binding domain Protein Sequence Similarity - PFAM Model Name: PF01568
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF14710 Respiratory nitrate reductase alpha N-terminal Protein Sequence Similarity - PFAM Model Name: PF14710
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR006963 Molybdopterin oxidoreductase, 4Fe-4S domain Protein Sequence Similarity - InterPro Domain: IPR006963
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR028189 Nitrate reductase, alpha subunit, N-terminal Protein Sequence Similarity - InterPro Domain: IPR028189
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR006655 Molybdopterin oxidoreductase, prokaryotic, conserved site Protein Sequence Similarity - InterPro Domain: IPR006655
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR009010 Aspartate decarboxylase-like domain superfamily Protein Sequence Similarity - InterPro Domain: IPR009010
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR006468 Nitrate reductase, alpha subunit Protein Sequence Similarity - InterPro Domain: IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Automated Process Uncurated EC number 1.-.-.- Published Annotation - Database Name: EC2GO: GO:0016491
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved EC number 1.7.99.4 Published Annotation - Swiss-Prot Accession Number: P19319 Feb 2006
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.4.2 (metabolism; energy production/transport; electron acceptor) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P19319 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945999 Published Annotation - Entrez Gene Database ID: 945999
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:1787741 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0004898 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG10648 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P19319 Published Annotation - Swiss-Prot Accession Number: P19319 Jan 2005
Person Approved function enzyme; Energy metabolism, carbon: Anaerobic respiration Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74550.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0042126 nitrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006656, IPR006657, IPR006468, IPR006963
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006655
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0009325 nitrate reductase complex GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO molecular function GO:0009054 (DEPRECATED) electron acceptor activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0043546 molybdopterin cofactor binding GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006657
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0008940 nitrate reductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006468
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0030151 molybdenum ion binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006657
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006656
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0001764 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0000060 narG CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0000078 narG CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000123 narG CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0000038 narG CDS
Ortholog
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002278 narZ CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0002935 narZ CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001482 CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0001443 narZ CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002874 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007943 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078018 narZ CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001638 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001901 CDS
Automated Process Uncurated Go view Escherichia coli E2348/69 (EPEC) BCI-0001718 narZ CDS
Person Uncurated Go view Escherichia coli E24377A (ETEC) ADO-0006089 narZ CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006918 narZ CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026018 narZ CDS
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0007979 narZ CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006229 narZ CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001914 narZ CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001377 narZ CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001509 narZ CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001778 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001776 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001732 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001706 narZ CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175839 narZ CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001586 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001705 narZ CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132041 narZ CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001693 narZ CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0001838 narZ pseudogene
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0001291 narZ CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0108099 narZ CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0083202 narZ CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0095196 narZ CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072990 narZ pseudogene
Person Uncurated Go view Shigella flexneri 301 ABT-0088062 narZ pseudogene
Person Approved Go view Shigella sonnei 046 ADD-0001804 narZ CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20326mutantTn5 InsertionRequest the StrainABE-0004898narZnarZ::Tn5KAN-2 at position 2321 in Minus orientation ,- pKD460Blattner Laboratory
FB20327mutantTn5 InsertionRequest the StrainABE-0004898narZnarZ::Tn5KAN-2 at position 2322 in Minus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1533850 to 1547590
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004877yddHCDScomplement15332821533851Downstream
ABE-0004882nhoACDSforward15340241534869Downstream
ABE-0285451repeat_regionforward15348831534957Downstream
ABE-0004886yddECDScomplement15349651535858Downstream
ABE-0004889narVCDScomplement15359371536617Downstream
ABE-0004892narWCDScomplement15366141537309Downstream
ABE-0285724matS12protein_bindforward15368071536819Downstream
ABE-0004896narYCDScomplement15373091538853Overlaps Downstream
ABE-0004898narZCDScomplement15388501542590Matches
ABE-0285452repeat_regionforward15426341542662Upstream
ABE-0004901narUCDScomplement15426721544060Upstream
ABE-0004904yddJpseudogenecomplement15443841545438Upstream
ABE-0004906yddKpseudogenecomplement15454281545753Upstream
ABE-0004910yddLpseudogenecomplement15457511546028Upstream
ABE-0004912yddGCDScomplement15462881547169Upstream
ABE-0004917fdnGCDSforward15474011550448Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.