Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0004901 (narU) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0004901 showshow history
Names
narU yddF JW1464 ECK1463 b1469
Product
nitrate/nitrite transporter
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1542672 1544060 1389 463
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR004737 (IPR004737 Nitrate transporter NarK/NarU-like) Apr 2008
Automated Process Approved IPR011701 (IPR011701 Major facilitator superfamily) Apr 2008
Automated Process Approved IPR036259 (IPR036259 MFS transporter superfamily) Jul 2018
Automated Process Approved PF07690 (PF07690 Major Facilitator Superfamily) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0015707 nitrite transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO biological process GO:0006807 nitrogen compound metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0015706 nitrate transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR011701
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0015698 inorganic anion transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0015113 nitrite transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0015103 inorganic anion transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0015112 nitrate transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name narU Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved synonym yddF Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym ECK1463 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW1464 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1469 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product nitrate/nitrite transporter Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name nitrite extrusion protein 2 Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name nitrate extrusion protein (MFS family) Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR036259 MFS transporter superfamily Protein Sequence Similarity - InterPro Domain: IPR036259
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF07690 Major Facilitator Superfamily Protein Sequence Similarity - PFAM Model Name: PF07690
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR011701 Major facilitator superfamily Protein Sequence Similarity - InterPro Domain: IPR011701
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR004737 Nitrate transporter NarK/NarU-like Protein Sequence Similarity - InterPro Domain: IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 1.8.3 (metabolism; metabolism of other compounds; nitrogen metabolism) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.2.A.1 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Major Facilitator Superfamily (MFS)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.145 (transport; substrate; nitrite) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12153 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P37758 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0004901 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P37758 Published Annotation - Swiss-Prot Accession Number: P37758 Jan 2005
Automated Process Approved db xref GI:1787743 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945799 Published Annotation - Entrez Gene Database ID: 945799
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved function transport; Transport of small molecules: Anions Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note nitrite extrusion protein 2; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006807 - nitrogen compound metabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAD13433.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0015707 nitrite transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO biological process GO:0006807 nitrogen compound metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0015706 nitrate transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR011701
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0015698 inorganic anion transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0015113 nitrite transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0015103 inorganic anion transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004737
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0015112 nitrate transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004737
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0001763 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003208 narU CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003376 narU CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000110 narK CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0000039 narK CDS
Ortholog
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002277 narU CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0002934 narU CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001483 CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0001444 narU CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002873 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007942 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078019 narU CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001639 narU CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001902 CDS
Automated Process Uncurated Go view Escherichia coli E2348/69 (EPEC) BCI-0001719 narU CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006090 narU CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006919 narU CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026017 narU CDS
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0007978 narU CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006230 narU CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001913 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001378 narU CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001510 narU CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001777 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001777 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001731 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001707 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175840 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001587 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001704 narU CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132042 narU CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001694 narU CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0001835 narU CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0001292 narU CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0108106 narU CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0083201 narU CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0095193 narU CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072989 narU CDS
Person Approved Go view Shigella flexneri 301 ABT-0088061 narU CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001874 narU CDS
Person Approved Go view Shigella sonnei 046 ADD-0001803 narU pseudogene
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20328mutantTn5 InsertionRequest the StrainABE-0004901narUnarU::Tn5KAN-2 at position 748 in Plus orientation ,+ pKD46 Insertion site is approximate0Blattner Laboratory
FB20329mutantTn5 InsertionRequest the StrainABE-0004901narUnarU::Tn5KAN-2 at position 748 in Plus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1537672 to 1549060
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0004896narYCDScomplement15373091538853Downstream
ABE-0004898narZCDScomplement15388501542590Downstream
ABE-0285452repeat_regionforward15426341542662Downstream
ABE-0004901narUCDScomplement15426721544060Matches
ABE-0004904yddJpseudogenecomplement15443841545438Upstream
ABE-0004906yddKpseudogenecomplement15454281545753Upstream
ABE-0004910yddLpseudogenecomplement15457511546028Upstream
ABE-0004912yddGCDScomplement15462881547169Upstream
ABE-0004917fdnGCDSforward15474011550448Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.