Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0005070 (tam) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0005070 showshow history
Names
tam yneD lsrE JW1512 ECK1512 b1519
Product
trans-aconitate methyltransferase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1607346 1608104 759 253
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR013216 (IPR013216 Methyltransferase type 11) Apr 2008
Automated Process Approved IPR029063 (IPR029063 S-adenosyl-L-methionine-dependent methyltransferase) Jul 2018
Automated Process Approved PF13649 (PF13649 Methyltransferase domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO cellular component GO:0005737 cytoplasm Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO molecular function GO:0030798 trans-aconitate 2-methyltransferase activity Experimental - PubMed ID: 10224113 Jan 2006
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name tam Experimental - PubMed ID: 10224113
Reference: Escherichia coli 		Aug 2002
Person Approved synonym JW1512 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym yneD Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym ECK1512 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym lsrE Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved locus tag b1519 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product trans-aconitate methyltransferase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name trans-aconitate 2-methyltransferase Experimental - PubMed ID: 10224113
Reference: Escherichia coli 		Jan 2006
Protein Family
Automated Process Approved protein family IPR013216 Methyltransferase type 11 Protein Sequence Similarity - InterPro Domain: IPR013216
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF13649 Methyltransferase domain Protein Sequence Similarity - PFAM Model Name: PF13649
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR029063 S-adenosyl-L-methionine-dependent methyltransferase Protein Sequence Similarity - InterPro Domain: IPR029063
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 2.1.1.144 Published Annotation - Swiss-Prot Accession Number: P76145 Feb 2006
Person Uncurated MultiFun 7.1 (location of gene products; cytoplasm) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.7.1 (metabolism; central intermediary metabolism; unassigned reversible reactions) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P76145 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946074 Published Annotation - Entrez Gene Database ID: 946074
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:1787798 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG13812 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0005070 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P76145 Published Annotation - Swiss-Prot Accession Number: P76145 Jan 2006
Person Approved function putative enzyme; Not classified Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved mutant phenotype tam null: no coordinate information; culture extracts from a tam null mutant do not possess methyltransferase activity using trans-aconitate, cis-aconitate, citrate, or DL-isocitrate as substrates Experimental - PubMed ID: 10224113
Reference: Escherichia coli K-12 MC1000 		Feb 2006
Automated Process Approved note trans-aconitate 2-methyltransferase; GO_component: GO:0005737 - cytoplasm Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved physical properties Molecular Weight:29 kD by SDS-PAGE Experimental - PubMed ID: 10224113
Reference: Escherichia coli K-12 MC1000 		Feb 2006
Person Approved physical properties Vmax:21.3 nmol/min/mg protein; for trans-aconitate Experimental - PubMed ID: 10224113
Reference: Escherichia coli K-12 MC1000 		Feb 2006
Person Approved physical properties Km:0.32 mM; for trans-aconitate Experimental - PubMed ID: 10224113
Reference: Escherichia coli K-12 MC1000 		Feb 2006
Automated Process Approved protein id AAC74592.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved regulation expression regulated by luxS; expression of tam is down-regulated in a luxS null mutant grown in the absence of glucose, as determined by RT-PCR and microarray data Experimental - PubMed ID: 16321939
Reference: Escherichia coli K-12 W3110 		Feb 2006
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0005737 cytoplasm Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO molecular function GO:0030798 trans-aconitate 2-methyltransferase activity Experimental - PubMed ID: 10224113 Jan 2006
Comparative to toptop
Author Approval Details Genome Feature Name Type
Homolog
Person Approved Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0239855 tam CDS
Person Approved Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0123658 tam CDS
Person Approved Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0031368 tam CDS
Person Approved Go view Yersinia pseudotuberculosis IP32953 ACZ-0002633 tam CDS
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0003942 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002795 tam CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002819 tam CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0007902 tam CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002968 tam CDS
Ortholog
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001837 CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002242 tam CDS
Person Uncurated Go view Dickeya dadantii 3937 ABF-0018602 tam CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0000505 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0002731 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003962 tam CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005549 tam CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001515 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0010118 tam CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001477 tam CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002839 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007880 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005384 tam CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005031 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078058 tam CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001691 tam CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001955 tam CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0005199 tam CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008017 tam CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001754 tam CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006158 tam CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0003394 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0005510 tam CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009875 tam CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0008056 tam CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007009 tam CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006972 tam CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0006885 tam CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0011449 tam CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0008115 tam CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008388 tam CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0006977 tam CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007080 tam CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025959 tam CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0009421 tam CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006277 tam CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001961 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001425 tam CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001559 tam CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001815 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001872 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001772 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001746 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175891 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001638 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001652 tam CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132096 tam CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0000692 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001745 tam CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0000926 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0001650 CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0004561 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0008487 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0002333 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0002555 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0002362 tam CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0001645 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0003127 tam CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0001027 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0010805 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0000788 CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0006165 tam CDS
Automated Process Uncurated Go view Shigella boydii BS512 ADJ-0007100 tam CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004196 CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0001872 CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072791 tam CDS
Person Approved Go view Shigella flexneri 301 ABT-0087892 tam CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001678 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0008196 tam CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0002264 tam CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0001301 tam CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0002584 CDS
Automated Process Uncurated Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0239855 tam CDS
Automated Process Uncurated Go view Yersinia pestis Angola (biovar Pestoides) ADI-0005884 tam CDS
Automated Process Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0002418 tam CDS
Automated Process Uncurated Go view Yersinia pestis B42003004 (biovar Antiqua) BAL-0007884 tam CDS
Automated Process Uncurated Go view Yersinia pestis CA88-4125 (biovar Orientalis) BAF-0000955 tam CDS
Automated Process Uncurated Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0123658 tam CDS
Automated Process Uncurated Go view Yersinia pestis E1979001 (biovar Antiqua) BAM-0004654 tam CDS
Automated Process Uncurated Go view Yersinia pestis F1991016 (biovar Orientalis) BAQ-0005553 tam CDS
Automated Process Uncurated Go view Yersinia pestis FV-1 (biovar Orientalis) BAK-0003299 CDS
Automated Process Uncurated Go view Yersinia pestis IP275 (biovar Orientalis) BAC-0006872 tam CDS
Automated Process Uncurated Go view Yersinia pestis K1973002 (biovar Mediaevalis) BAO-0008311 tam CDS
Automated Process Uncurated Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0031368 tam CDS
Automated Process Uncurated Go view Yersinia pestis MG05-1020 (biovar Orientalis) BAP-0008254 tam CDS
Automated Process Uncurated Go view Yersinia pestis Nepal516 (biovar Antiqua) BAD-0002536 tam CDS
Automated Process Uncurated Go view Yersinia pestis Pestoides F (biovar Pestoides) BAI-0002305 CDS
Automated Process Uncurated Go view Yersinia pestis UG05-0454 (biovar Antiqua) BAN-0005817 tam CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0005464 tam CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0002633 tam CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0003151 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0001883 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0013784 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003778 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22192mutantTn5 InsertionRequest the StrainABE-0005070tamb1519::Tn5KAN-I-SceI at position 378 in Minus orientation ,+ pKD460Blattner Laboratory
FB23122mutantTn5 InsertionRequest the StrainABE-0005070tamb1519::Tn5KAN-I-SceI at position 378 in Minus orientation ,- pKD465Tim Durfee (durf@genome.wisc.edu)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1602346 to 1613104
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0005056lsrACDSforward16014901603025Upstream
ABE-0005059lsrCCDSforward16030191604047Upstream
ABE-0005061lsrDCDSforward16040471605039Upstream
ABE-0005063lsrBCDSforward16050511606073Upstream
ABE-0005065lsrFCDSforward16061001606975Upstream
ABE-0005067lsrGCDSforward16069991607289Upstream
ABE-0005070tamCDSforward16073461608104Matches
ABE-0005072yneECDScomplement16081081609022Downstream
ABE-0005076uxaBCDScomplement16092291610680Downstream
ABE-0005081dgcFpseudogenecomplement16109071611892Downstream
ABE-0005084yneGCDScomplement16119661612325Downstream
ABE-0005086glsBCDScomplement16123251613251Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.