Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0005116 (eamA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0005116 showshow history
Names
eamA JW5250 ECK1526 ydeD b1533
Product
cysteine and O-acetyl-L-serine efflux system
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1620238 1621137 900 300
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000620 (IPR000620 EamA domain) Apr 2008
Automated Process Approved PF00892 (PF00892 EamA-like transporter family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000620
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000620
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name eamA Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Aug 2002
Person Approved synonym ydeD Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK1526 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW5250 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1533 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product cysteine and O-acetyl-L-serine efflux system Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name amino acid metabolite efflux pump Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name cysteine pathway metabolite transport protein (MFS family) Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR000620 EamA domain Protein Sequence Similarity - InterPro Domain: IPR000620
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00892 EamA-like transporter family Protein Sequence Similarity - PFAM Model Name: PF00892
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated MultiFun 4.2.A.1 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Major Facilitator Superfamily (MFS)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.40 (transport; substrate; cysteine/O-acetyl-L-serine/cysteine metabolites) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11639 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0005116 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:87081929 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P31125 Published Annotation - Swiss-Prot Accession Number: P31125 Jan 2005
Automated Process Approved db xref UniProtKB/Swiss-Prot:P31125 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946081 Published Annotation - Entrez Gene Database ID: 946081
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved note amino acid metabolite efflux pump Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74606.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000620
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000620
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002338 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002585 eamA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002416 eamA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004067 eamA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002294 eamA CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0002073 eamA CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001880 CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002224 eamA_1 CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0002868 eamA_2 CDS
Person Uncurated Go view Dickeya dadantii 3937 ABF-0017608 eamA CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0000077 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0002695 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003940 eamA CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005564 CDS
Automated Process Uncurated Go view Escherichia coli 536 (UPEC) AEF-0001531 CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0001495 ydeD CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002818 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007865 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005404 eamA CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005007 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078076 ydeD CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001979 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0005178 eamA CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008042 eamA CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001767 eamA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006179 eamA CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0003409 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0005526 eamA CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009890 eamA CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0008071 eamA CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006994 eamA CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006987 eamA CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0006870 eamA CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0011464 eamA CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0008130 eamA CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008373 eamA CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0006962 eamA CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007065 eamA CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025942 ydeD CDS
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0009404 eamA CDS
Automated Process Uncurated Go view Escherichia coli HS ADR-0006297 eamA CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001438 eamA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001573 eamA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001839 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001788 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001658 CDS
Automated Process Uncurated Go view Escherichia coli SMS-3-5 AFT-0001630 eamA CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132110 eamA CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0000676 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001765 ydeD CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0001626 eamA CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0004526 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0009025 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0003168 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0003358 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0003316 eamA CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0000668 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0000080 eamA CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0000012 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0007783 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0003507 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0286304 eamA CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0007058 yded3 CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0064722 eamA CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0002908 CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0002287 eamA CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0004495 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0005827 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0001785 ydeD CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006088 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0004985 ydeD CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0004845 ydeD CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0004959 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006052 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0004989 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0005038 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0004499 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0005120 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0005997 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0004789 CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0001798 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006240 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0005384 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0005709 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0005040 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0108193 CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0083152 CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0001837 ydeD CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0095032 ydeD CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007845 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0005696 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0007049 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0004453 CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0001837 ydeD CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072776 ydeD CDS
Person Approved Go view Shigella flexneri 301 ABT-0087878 ydeD CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001697 ydeD CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0010616 CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0002272 eamA CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0013690 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003807 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20352mutantTn5 InsertionRequest the StrainABE-0005116eamAydeD::Tn5KAN-I-SceI at position 304 in Minus orientation ,+ pKD460Blattner Laboratory
FB20353mutantTn5 InsertionRequest the StrainABE-0005116eamAydeD::Tn5KAN-I-SceI at position 304 in Minus orientation ,- pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1615238 to 1626137
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0005096yneJCDSforward16148041615685Downstream
ABE-0005100yneKCDSforward16157631616878Downstream
ABE-0005103ydeACDSforward16170281618218Downstream
ABE-0005106marCCDScomplement16182431618908Downstream
ABE-0005110marRCDSforward16191201619554Downstream
ABE-0005112marACDSforward16195741619957Downstream
ABE-0285727matS15protein_bindforward16199661619978Downstream
ABE-0005114marBCDSforward16199891620207Downstream
ABE-0005116eamACDScomplement16202381621137Matches
ABE-0005121ydeECDSforward16213321622519Upstream
ABE-0285183mgtSCDSforward16226461622741Upstream
ABE-0285749mgtTCDSforward16227411622845Upstream
ABE-0285266mgrRncRNAcomplement16228171622914Upstream
ABE-0005123dgcZCDScomplement16229601623850Upstream
ABE-0005127ydeICDScomplement16241051624497Upstream
ABE-0005131ydeJCDSforward16247731625291Upstream
ABE-0005133dcpCDScomplement16253351627380Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.