Escherichia coli K-12 MG1655
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0005137 (ydfG) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0005137 showshow history
Names
ydfG JW1532 ECK1532 b1539
Product
3-hydroxy acid dehydrogenase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1627517 1628263 747 249
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR002198 (IPR002198 (DEPRECATED) Short-chain dehydrogenase/reductase SDR) Apr 2008
Automated Process Approved IPR002347 (IPR002347 Short-chain dehydrogenase/reductase SDR) Apr 2008
Automated Process Approved IPR036291 (IPR036291 NAD(P)-binding domain superfamily) Jul 2018
Automated Process Approved PF00106 (PF00106 short chain dehydrogenase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002198
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002198
Reference: Escherichia coli K-12 MG1655
Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name ydfG Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Person Approved synonym ECK1532 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW1532 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1539 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product 3-hydroxy acid dehydrogenase Published Annotation - EcoCyc Accession Number: EG12345
Reference: Escherichia coli K-12 MG1655
Oct 2021
Person Approved alternate product name L-allo-threonine dehydrogenase, NAD(P)-binding Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name putative oxidoreductase, NAD(P)-binding Published Annotation - Database Name: GenProtEC Jan 2006
Automated Process Approved alternate product name malonic semialdehyde reductase, NADPH-dependent; L-allo-threonine dehydrogenase, NAD(P)-dependent; also oxidizes L-serine, D-serine, D-threonine and 3-hydroxyisobutyrate Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved alternate product name putative oxidoreductase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Jan 2006
Protein Family
Automated Process Approved protein family IPR002198 (DEPRECATED) Short-chain dehydrogenase/reductase SDR Protein Sequence Similarity - InterPro Domain: IPR002198
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved protein family PF00106 short chain dehydrogenase Protein Sequence Similarity - PFAM Model Name: PF00106
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR002347 Short-chain dehydrogenase/reductase SDR Protein Sequence Similarity - InterPro Domain: IPR002347
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved protein family IPR036291 NAD(P)-binding domain superfamily Protein Sequence Similarity - InterPro Domain: IPR036291
Reference: Escherichia coli K-12 MG1655
Jul 2018
Other
Person Approved EC number 1.1.1.- Published Annotation - Swiss-Prot Accession Number: P39831 Feb 2006
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P39831 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GI:1787820 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved db xref UniProtKB:P39831 Published Annotation - Swiss-Prot Accession Number: P39831 Jan 2005
Automated Process Approved db xref ASAP:ABE-0005137 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GeneID:946085 Published Annotation - Entrez Gene Database ID: 946085
Reference: Escherichia coli K-12 MG1655
Jun 2009
Automated Process Approved db xref EcoGene:EG12345 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved function putative enzyme; Not classified Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Automated Process Approved note putative oxidoreductase Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved protein id AAC74612.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.26
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655
Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002198
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002198
Reference: Escherichia coli K-12 MG1655
Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0001904 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0001937 ydfG CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0001539 ydfG CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002494 ydfG CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0000201 ydfG CDS
Ortholog
Automated Process Uncurated Go view Brenneria salicis ATCC 15712 [T] AMK-0002751 CDS
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0001959 CDS
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0002307 ydfG CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001826 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002210 ydfG CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0002857 ydfG CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0006921 CDS
Person Uncurated Go view Dickeya dadantii 3937 ABF-0020763 ydfG CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0001997 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0002541 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0301484 ydfG CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC BAA-2158 APN-0001911 ydfG CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0001811 ydfG CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003933 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005570 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001535 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0010141 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001499 ydfG CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002813 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007857 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005410 CDS
Person Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005000 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078080 ydfG CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001720 ydfG CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0001986 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0005172 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008048 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001773 ydfG CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006186 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0003418 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0005535 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009899 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0008081 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0009906 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0006995 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0006861 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0011473 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0010599 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008364 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0006953 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007059 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0010733 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0025933 ydfG CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0009399 ydfG CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006304 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001936 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001444 ydfG CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001579 ydfG CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001848 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001839 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001795 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001769 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175916 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001664 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001625 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132118 ydfG CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0000667 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001769 ydfG CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0001692 CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0002912 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0001594 ydfG CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0004507 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0006620 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0001298 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0003746 CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0002773 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0002028 ydfG CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0002192 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0009719 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0001861 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0290105 ydfG CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0005135 ydfG CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0062516 CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0001416 CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0001772 CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0002353 CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0006936 CDS
Person Uncurated Go view Photorhabdus luminescens subsp. laumondii TTO1 [T] ACD-0141940 CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0004990 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0005834 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0001502 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0001778 ydfG CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006096 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0005004 ydfG CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0004862 ydfG CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006045 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0004981 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0005030 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0004492 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0005113 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0005990 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0004796 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0001353 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0001344 ydfG CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0001809 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006233 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0005391 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0005701 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0005033 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0108203 CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0083146 CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0001827 ydfG CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0095013 ydfG CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007852 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0005689 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0008548 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0003503 CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0267784 CDS
Person Approved Go view Shigella boydii 227 ADV-0001786 ydfG CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0007111 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004222 CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0001828 ydfG CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072768 ydfG CDS
Person Approved Go view Shigella flexneri 301 ABT-0087873 ydfG CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001651 ydfG CDS
Person Approved Go view Shigella sonnei 046 ADD-0001723 ydfG CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0004129 ydfG CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0003471 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0005981 CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0002977 ydfG CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0000621 ydfG CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0000424 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0005835 CDS
Person Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0002279 ydfG CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0002337 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0013658 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003814 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)

Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20361mutantTn5 InsertionRequest the StrainABE-0005137ydfGydfG::Tn5KAN-I-SceI at position 338 in Minus orientation ,+ pKD460Blattner Laboratory
FB20362mutantTn5 InsertionRequest the StrainABE-0005137ydfGydfG::Tn5KAN-I-SceI at position 338 in Minus orientation ,- pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1622517 to 1633263
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0005121ydeECDSforward16213321622519Upstream
ABE-0285183mgtSCDSforward16226461622741Upstream
ABE-0285749mgtTCDSforward16227411622845Upstream
ABE-0285266mgrRncRNAcomplement16228171622914Upstream
ABE-0005123dgcZCDScomplement16229601623850Upstream
ABE-0005127ydeICDScomplement16241051624497Upstream
ABE-0005131ydeJCDSforward16247731625291Upstream
ABE-0005133dcpCDScomplement16253351627380Upstream
ABE-0005137ydfGCDSforward16275171628263Matches
ABE-0005144rspRCDSforward16283521629038Downstream
ABE-0005147ydfZCDSforward16292151629418Downstream
ABE-0005149ydfICDScomplement16294531630913Downstream
ABE-0285728matS16protein_bindforward16303241630336Downstream
ABE-0005152ydfJ_2CDSpcomplement16310021632285Downstream
ABE-0285155ydfJpseudogenecomplement16310021632276(Interval #2) Downstream
ABE-0234209Qin (Kim)prophageforward16322861652754Downstream
ABE-0285457cryptic prophage Qin/Kimmisc_featurecomplement16322861652743Downstream
ABE-0285766ynfTCDSforward16328901633003Downstream
ABE-0005161ydfKCDSforward16330721633305Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.