Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0005309 (ynfG) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0005309 showshow history
Names
ynfG JW1581 ECK1584 b1589
Product
putative oxidoreductase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1662990 1663607 618 206
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR014297 (IPR014297 Dimethylsulphoxide reductase, chain B) Apr 2008
Automated Process Approved IPR001450 (IPR001450 (DEPRECATED) 4Fe-4S binding domain) Apr 2008
Automated Process Approved IPR017896 (IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain) Jul 2018
Automated Process Approved PF13247 (PF13247 4Fe-4S dicluster domain) Jul 2018
Automated Process Approved PF12800 (PF12800 4Fe-4S binding domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0009055 electron transfer activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name ynfG Published Annotation - Swiss-Prot Accession Number: P77313 Aug 2002
Person Approved synonym ECK1584 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW1581 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1589 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product putative oxidoreductase Published Annotation - EcoCyc Accession Number: G6847
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Approved alternate product name oxidoreductase, Fe-S subunit Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name putative dimethyl sulfoxide reductase, Fe-S subunit Published Annotation - Swiss-Prot Accession Number: P77313 Jan 2006
Protein Family
Automated Process Approved protein family PF12800 4Fe-4S binding domain Protein Sequence Similarity - PFAM Model Name: PF12800
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR014297 Dimethylsulphoxide reductase, chain B Protein Sequence Similarity - InterPro Domain: IPR014297
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR001450 (DEPRECATED) 4Fe-4S binding domain Protein Sequence Similarity - InterPro Domain: IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain Protein Sequence Similarity - InterPro Domain: IPR017896
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF13247 4Fe-4S dicluster domain Protein Sequence Similarity - PFAM Model Name: PF13247
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG13845 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0005309 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945638 Published Annotation - Entrez Gene Database ID: 945638
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P0AAJ1 Published Annotation - Swiss-Prot Accession Number: P0AAJ1 Jan 2006
Automated Process Approved db xref GI:1787872 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0AAJ1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function putative enzyme; Not classified Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved protein id AAC74661.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 7.88
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0009055 electron transfer activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0004022 ynfG CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0000103 ynfG CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004961 ynfG CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003643 ynfG CDS
Paralog
Person Uncurated Go view Escherichia coli K-12 MG1655 ABE-0003046 dmsB CDS
Ortholog
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002197 dmsB_4 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0004132 dmsB_1 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005618 dmsB_1 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0010154 dmsB_2 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001512 dmsB CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002799 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007771 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005425 dmsB_1 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0006051 pseudogene
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078094 ynfG CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001791 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002072 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0005100 dmsB_1 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008701 dmsB_1 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001787 ynfG CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006233 dmsB2 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001769 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0005677 dmsB_2 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0009985 dmsB_2 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0009930 dmsB_1 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0008703 dmsB_1 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0007138 dmsB_2 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0008419 dmsB_1 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0010970 dmsB_2 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0009400 dmsB_1 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0011044 dmsB_1 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009271 dmsB_1 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0008561 dmsB_1 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0009688 dmsB_1 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026376 ynfG CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006337 dmsB1 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0001989 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001491 ynfG CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001631 ynfG CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001940 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001944 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002327 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001784 CDS
Person Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0179624 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001739 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001610 dmsB_1 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132276 ynfG CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004290 CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001783 ynfG CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0001759 ynfG pseudogene
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0001357 ynfG CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0108237 ynfG CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0083132 ynfG CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0094978 ynfG CDS
Person Approved Go view Shigella boydii 227 ADV-0005140 ynfG pseudogene
Person Approved Go view Shigella dysenteriae 197 ADU-0001807 ynfG CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072828 ynfG CDS
Person Approved Go view Shigella flexneri 301 ABT-0255988 ynfG CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001711 CDS
Person Approved Go view Shigella sonnei 046 ADD-0001707 ynfG CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22214mutantTn5 InsertionRequest the StrainABE-0005309ynfGb1589::Tn5KAN-I-SceI at position 258 in Minus orientation ,+ pKD460Blattner Laboratory
FB23140mutantTn5 InsertionRequest the StrainABE-0005309ynfGb1589::Tn5KAN-I-SceI at position 258 in Minus orientation ,- pKD465Tim Durfee (durf@genome.wisc.edu)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1657990 to 1668607
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0005302ynfECDSforward16580691660495Upstream
ABE-0005307ynfFCDSforward16605561662979Upstream
ABE-0005309ynfGCDSforward16629901663607Matches
ABE-0005311ynfHCDSforward16636091664463Downstream
ABE-0005314dmsDCDSforward16645061665120Downstream
ABE-0285460repeat_regionforward16651361665166Downstream
ABE-0285729matS17protein_bindforward16651751665187Downstream
ABE-0005317clcBCDSforward16653151666571Downstream
ABE-0005319bidACDScomplement16665241667219Downstream
ABE-0005323mlcCDScomplement16673441668564Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.