Escherichia coli K-12 MG1655
About ASAP Data Release Policy University of Wisconsin—Madison
Home    Overview    Annotations    BLAST    Downloads    Comparative Genomics    Experiments    Strains    Help (this script)    User: guest  / Log On   
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0005435 (add) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0005435 showshow history
Names
add JW1615 ECK1618 b1623
Product
adenosine deaminase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1702233 1703234 1002 334
Sequence

Get full DNA or protein sequence

View sequence with flanking regions: bases upstream and bases downstream

Context

Browse sequence in GaPP

You last hovered over feature (none)
Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR006330 (IPR006330 Adenosine/adenine deaminase) Apr 2008
Automated Process Approved IPR001365 (IPR001365 Adenosine/AMP deaminase domain) Apr 2008
Automated Process Approved IPR006650 (IPR006650 Adenosine/AMP deaminase active site) Apr 2008
Automated Process Approved IPR032466 (IPR032466 Metal-dependent hydrolase) Jul 2018
Automated Process Approved PF00962 (PF00962 Adenosine deaminase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0009168 purine ribonucleoside monophosphate biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006330
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0015949 nucleobase-containing small molecule interconversion Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0019239 deaminase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001365
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0004000 adenosine deaminase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006330
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name add Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK1618 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW1615 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1623 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product adenosine deaminase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name adenosine deaminase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR006330 Adenosine/adenine deaminase Protein Sequence Similarity - InterPro Domain: IPR006330
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR032466 Metal-dependent hydrolase Protein Sequence Similarity - InterPro Domain: IPR032466
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR006650 Adenosine/AMP deaminase active site Protein Sequence Similarity - InterPro Domain: IPR006650
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR001365 Adenosine/AMP deaminase domain Protein Sequence Similarity - InterPro Domain: IPR001365
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00962 Adenosine deaminase Protein Sequence Similarity - PFAM Model Name: PF00962
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 3.5.4.4 Published Annotation - Swiss-Prot Accession Number: P22333 Feb 2006
Person Uncurated MultiFun 1.7.33 (metabolism; central intermediary metabolism; nucleotide and nucleoside conversions) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945851 Published Annotation - Entrez Gene Database ID: 945851
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P22333 Published Annotation - Swiss-Prot Accession Number: P22333 Jan 2005
Automated Process Approved db xref EcoGene:EG10030 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P22333 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0005435 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1787910 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Salvage of nucleosides and nucleotides Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74695.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0009168 purine ribonucleoside monophosphate biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006330
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0015949 nucleobase-containing small molecule interconversion Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0019239 deaminase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001365
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0004000 adenosine deaminase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006330
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0001907 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002068 add CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0001954 add CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0001905 add CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0001316 add CDS
Ortholog
Automated Process Uncurated Go view Brenneria salicis ATCC 15712 [T] AMK-0002766 CDS
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0001972 CDS
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0001923 add CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0002243 add CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002164 CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0002803 CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0006935 CDS
Person Uncurated Go view Dickeya dadantii 3937 ABF-0015574 add CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0001986 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0002431 add CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0301443 add CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC BAA-2158 APN-0001896 add CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0001825 add CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0004167 add CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005653 add CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001579 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0010189 add CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001546 add CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002765 add CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007727 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005459 add CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0006017 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078130 add CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001827 add CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002107 add CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008820 add CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008736 add CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001822 add CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006268 add CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001806 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0005714 add CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0010022 add CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0009966 add CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0008669 add CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0007175 add CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0008453 add CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0010933 add CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0009362 add CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0010577 add CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009233 add CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0008597 add CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0009724 add CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026412 add CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0008631 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006373 add CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002024 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001525 add CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001665 add CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001902 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0001978 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002289 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001820 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0175971 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001773 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001575 add CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132312 add CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004254 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001818 add CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0001607 add CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0002932 add CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0001963 add CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0004347 add CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0006632 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0001178 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0003922 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0001192 add CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0002923 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0001893 add CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0002333 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0009511 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0002591 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0289253 add CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0005018 add CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0062530 add CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0004329 CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0003867 add CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0000550 add CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0006917 CDS
Person Uncurated Go view Photorhabdus luminescens subsp. laumondii TTO1 [T] ACD-0142244 add CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0005885 add CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0001574 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0001714 add CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006148 add CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0005182 add CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0005014 add CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0008920 add CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0005996 add CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0004932 add CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0004975 add CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0004441 add CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0005061 add CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0005940 add CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0004849 add CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0001390 add misc_feature
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0001868 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006184 add CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0006801 add CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0005648 add CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0004980 add CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0108381 add CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0083045 add CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0001765 add CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0094890 add CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007903 add CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0005639 add CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0008357 add CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0003539 add CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0268391 add CDS
Person Approved Go view Shigella boydii 227 ADV-0001668 add CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0007220 add CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004290 add CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0002134 add CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072868 add CDS
Person Approved Go view Shigella flexneri 301 ABT-0087958 add CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001748 add CDS
Person Approved Go view Shigella sonnei 046 ADD-0001663 add CDS
Person Uncurated Go view Vibrio cholerae N16961 ADH-0002850 VC2751 CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0004155 add CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0002783 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0005915 add CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0003005 add CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0003351 add CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0000405 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0005849 add CDS
Person Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0002264 add CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0002673 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0002356 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003704 add CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1697233 to 1708234
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0005419hdhACDScomplement16972731698040Upstream
ABE-0005421malICDScomplement16981521699180Upstream
ABE-0005429malXCDSforward16993551700947Upstream
ABE-0005432malYCDSforward17009571702129Upstream
ABE-0005435addCDSforward17022331703234Matches
ABE-0005437ydgJCDScomplement17032681704308Downstream
ABE-0285730matS18protein_bindforward17040021704014Downstream
ABE-0047212blrCDSforward17045511704676Downstream
ABE-0005443cnuCDSforward17049491705164Downstream
ABE-0005447ydgKCDSforward17052501705690Downstream
ABE-0005450rsxACDSforward17057671706348Downstream
ABE-0005452rsxBCDSforward17063481706926Downstream
ABE-0005454rsxCCDSforward17069191709141Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.