Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0005748 (pfkB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0005748 showshow history
Names
pfkB JW5280 ECK1721 b1723
Product
6-phosphofructokinase II
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 1806370 1807299 930 310
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR011611 (IPR011611 Carbohydrate kinase PfkB) Apr 2008
Automated Process Approved IPR002173 (IPR002173 Carbohydrate/purine kinase, PfkB, conserved site) Apr 2008
Automated Process Approved IPR017583 (IPR017583 Tagatose/fructose phosphokinase) Jul 2018
Automated Process Approved IPR029056 (IPR029056 Ribokinase-like) Jul 2018
Automated Process Approved PF00294 (PF00294 pfkB family carbohydrate kinase) Jul 2018
Automated Process Uncurated GH28 (GH28 Glycoside Hydrolase Family 28) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0005975 carbohydrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR017583
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0005737 cytoplasm Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0016773 phosphotransferase activity, alcohol group as acceptor GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR017583
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name pfkB Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK1721 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW5280 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1723 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product 6-phosphofructokinase II Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name 6-phosphofructokinase II Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name 6-phosphofructokinase II; suppressor of pfkA Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Uncurated protein family GH28 Glycoside Hydrolase Family 28 Protein Sequence Similarity - CAZy Model Name: GH28
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR011611 Carbohydrate kinase PfkB Protein Sequence Similarity - InterPro Domain: IPR011611
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00294 pfkB family carbohydrate kinase Protein Sequence Similarity - PFAM Model Name: PF00294
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR029056 Ribokinase-like Protein Sequence Similarity - InterPro Domain: IPR029056
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR017583 Tagatose/fructose phosphokinase Protein Sequence Similarity - InterPro Domain: IPR017583
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR002173 Carbohydrate/purine kinase, PfkB, conserved site Protein Sequence Similarity - InterPro Domain: IPR002173
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Automated Process Uncurated EC number 2.7.1.- Published Annotation - Database Name: EC2GO: GO:0016773
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved EC number 2.7.1.11 Published Annotation - Swiss-Prot Accession Number: P06999 Feb 2006
Person Uncurated MultiFun 7.1 (location of gene products; cytoplasm) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.1.1 (metabolism; carbon utilization; carbon compounds) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946230 Published Annotation - Entrez Gene Database ID: 946230
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P06999 Published Annotation - Swiss-Prot Accession Number: P06999 Jan 2005
Automated Process Approved db xref GI:1788017 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P06999 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0005748 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG10700 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Energy metabolism, carbon: Glycolysis Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note 6-phosphofructokinase II; suppressor of pfkA; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74793.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.26
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0005975 carbohydrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR017583
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO cellular component GO:0005737 cytoplasm Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0016773 phosphotransferase activity, alcohol group as acceptor GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR017583
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0004819 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002797 pfkB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002855 pfkB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002692 pfkB CDS
Ortholog
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0002926 CDS
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0001753 pfkB CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0002379 pfkB CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002044 pfkB CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0002634 pfkB CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0002309 pfkB CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0004279 pfkB CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0007773 pfkB CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001682 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0010300 pfkB CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001634 pfkB CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002666 pfkB CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007609 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0008129 pfkB CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009532 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078240 pfkB CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001934 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002260 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0009683 pfkB CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001972 pfkB CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006380 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0006235 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0005825 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0010128 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0008688 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007573 pfkB CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0007281 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007617 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0010826 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0008665 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0009918 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007788 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007804 pfkB CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008433 pfkB CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026511 pfkB CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0006740 pfkB CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006479 pfkB CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002128 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001619 pfkB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001770 pfkB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0002348 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002084 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002182 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001929 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0176086 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001923 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001467 pfkB CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132412 pfkB CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004148 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001923 pfkB CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0002189 pfkB CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0004235 pfkB CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006019 pfkB CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0001720 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0001572 pfkB CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006287 pfkB CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0005612 pfkB CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0005428 pfkB CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0008226 pfkB CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0005819 pfkB CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0008045 pfkB CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0004835 pfkB CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0007585 pfkB CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0004923 pfkB CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0005807 pfkB CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0004984 pfkB CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0001527 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0001518 pfkB CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0002026 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006047 pfkB CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0007495 pfkB CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0005511 pfkB CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0008175 pfkB CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0108644 pfkB CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0082920 pfkB CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0001612 pfkB CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0094512 pfkB CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0008109 pfkB CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0008164 pfkB CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006145 pfkB CDS
Person Approved Go view Shigella boydii 227 ADV-0001513 pfkB CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0007379 pfkB CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0008125 pfkB CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0002098 pfkB CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0072703 pfkB CDS
Person Approved Go view Shigella flexneri 301 ABT-0087824 pfkB CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0001592 pfkB CDS
Person Approved Go view Shigella sonnei 046 ADD-0001556 pfkB CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0003468 fruK_2 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0014543 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003583 pfkB CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20438mutantTn5 InsertionRequest the StrainABE-0005748pfkBpfkB::Tn5KAN-2 at position 526 in Plus orientation ,+ pKD460Blattner Laboratory
FB20439mutantTn5 InsertionRequest the StrainABE-0005748pfkBpfkB::Tn5KAN-2 at position 526 in Plus orientation ,- pKD460Blattner Laboratory
FB23556mutantTn5 InsertionRequest the StrainABE-0005748pfkBpfkB::Tn5KAN-I-SceI at position 684 in Plus orientation ,- pKD46 Saved as well because of the ISCE site0Blattner Laboratory
FB23675mutantTn5 InsertionRequest the StrainABE-0005748pfkBpfkB::Tn5KAN-I-SceI at position 684 in Plus orientation ,- pKD46 Saved as well because of the ISCE site0Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1801370 to 1812299
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0005736thrSCDScomplement18006421802570Upstream
ABE-0005740arpB_1CDSpforward18030941803567Upstream
ABE-0285095arpBpseudogeneforward18030941804993Upstream
ABE-0005742arpB_2CDSpforward18035781804993Upstream
ABE-0285056yniDCDSforward18051651805272Upstream
ABE-0005744ydiYCDScomplement18053251806083Upstream
ABE-0005748pfkBCDSforward18063701807299Matches
ABE-0005751ydiZCDSforward18074001807690Downstream
ABE-0005755yniACDSforward18077961808656Downstream
ABE-0005757yniBCDScomplement18086971809233Downstream
ABE-0005761hxpBCDSforward18093801810048Downstream
ABE-0005768ydjMCDSforward18102111810801Downstream
ABE-0005771tcyPCDSforward18109341812325Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.