Escherichia coli K-12 MG1655
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0005822 (astA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0005822 showshow history
Names
astA ydjV JW1736 ECK1745 b1747
Product
arginine succinyltransferase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1829731 1830765 1035 345
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR007041 (IPR007041 Arginine N-succinyltransferase AstA/AruG) Apr 2008
Automated Process Approved IPR017650 (IPR017650 Arginine N-succinyltransferase) Jul 2018
Automated Process Approved IPR016181 (IPR016181 Acyl-CoA N-acyltransferase) Jul 2018
Automated Process Approved PF04958 (PF04958 Arginine N-succinyltransferase beta subunit) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0009063 cellular amino acid catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006527 arginine catabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007041
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Approved GO molecular function GO:0008791 arginine N-succinyltransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007041
Reference: Escherichia coli K-12 MG1655
Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name astA Published Annotation - Swiss-Prot Accession Number: P76218 Aug 2002
Person Approved synonym ECK1745 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym ydjV Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved synonym JW1736 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1747 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product arginine succinyltransferase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name arginine succinyltransferase Published Annotation - Swiss-Prot Accession Number: P76218 Jan 2006
Protein Family
Automated Process Approved protein family IPR007041 Arginine N-succinyltransferase AstA/AruG Protein Sequence Similarity - InterPro Domain: IPR007041
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved protein family IPR016181 Acyl-CoA N-acyltransferase Protein Sequence Similarity - InterPro Domain: IPR016181
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR017650 Arginine N-succinyltransferase Protein Sequence Similarity - InterPro Domain: IPR017650
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family PF04958 Arginine N-succinyltransferase beta subunit Protein Sequence Similarity - PFAM Model Name: PF04958
Reference: Escherichia coli K-12 MG1655
Jul 2018
Other
Person Approved EC number 2.3.1.109 Published Annotation - Swiss-Prot Accession Number: P0AE37 Feb 2006
Person Uncurated MultiFun 1.1.3 (metabolism; carbon utilization; amino acids) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref EcoGene:EG13998 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GI:1788043 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0AE37 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved db xref UniProtKB:P0AE37 Published Annotation - Swiss-Prot Accession Number: P0AE37 Jan 2006
Automated Process Approved db xref ASAP:ABE-0005822 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GeneID:946261 Published Annotation - Entrez Gene Database ID: 946261
Reference: Escherichia coli K-12 MG1655
Jun 2009
Automated Process Approved function enzyme; Not classified Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved note GO_process: GO:0009063 - cellular amino acid catabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved protein id AAC74817.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655
Feb 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655
Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655
Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006527 arginine catabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007041
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO biological process GO:0009063 cellular amino acid catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Approved GO molecular function GO:0008791 arginine N-succinyltransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007041
Reference: Escherichia coli K-12 MG1655
Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0004275 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002113 astA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002088 astA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0001378 astA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002018 astA CDS
Ortholog
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0002219 CDS
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0001727 astA CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0002398 astA CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002020 aruG CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0002611 aruG CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0002285 astA CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0301129 astA CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC BAA-2158 APN-0001792 astA CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0001922 astA CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0004310 astA CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0007797 astA CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001705 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0010324 astA CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001657 astA CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002643 astA CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007581 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0008157 astA CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009559 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078266 astA CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0001961 astA CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002288 astA CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0007321 astA CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0009708 astA CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0001995 astA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006406 astA CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0006260 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0005849 astA CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0010152 astA CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0008712 astA CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007549 astA CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0007305 astA CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007593 astA CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0010802 astA CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0008689 astA CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0009942 astA CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007762 astA CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007780 astA CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008457 astA CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026535 astA CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0006717 astA CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006505 astA CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002153 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001643 astA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001794 astA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0002322 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002157 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0001954 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0001948 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001443 astA CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132436 astA CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004121 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0001949 astA CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0001922 astA CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0001226 astA CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0004210 astA CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0004915 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0001035 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0004064 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0001048 astA CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0003085 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0001734 astA CDS
Automated Process Approved Go view Pantoea ananatis PA13 APK-0002488 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0009378 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0002451 CDS
Automated Process Approved Go view Pantoea stewartii DC283 ACV-0289762 astA CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0004877 astA CDS
Person Uncurated Go view Photorhabdus luminescens subsp. laumondii TTO1 [T] ACD-0143960 astA CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0001868 aruG CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006043 astA CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0001745 CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006311 astA CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0005674 astA CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0008250 astA CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0005795 astA CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0008021 astA CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0004811 astA CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0007561 astA CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0004899 astA CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0005783 astA CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0005008 astA CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0001549 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0001540 astA CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0002051 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006024 astA CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0007518 astA CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0005487 astA CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0008151 astA CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0108687 astA CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0001585 astA CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0094446 astA CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0008132 astA CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0008141 astA CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006168 astA CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0002921 astA CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0264173 astA CDS
Person Approved Go view Shigella boydii 227 ADV-0001486 CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0007405 astA CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0006129 astA CDS
Person Uncurated Go view Shigella dysenteriae 197 ADU-0001765 CDS
Person Uncurated Go view Shigella flexneri 2457T ABQ-0072672 CDS
Person Uncurated Go view Shigella flexneri 301 ABT-0255979 CDS
Automated Process Uncurated Go view Shigella flexneri 8401 BAG-0001568 CDS
Person Approved Go view Shigella sonnei 046 ADD-0001531 CDS
Person Uncurated Go view Vibrio cholerae N16961 ADH-0002716 VC2617 CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0000371 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0007081 astA CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0000782 astA CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0000294 astA CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0000108 CDS
Person Uncurated Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0238400 astA CDS
Automated Process Uncurated Go view Yersinia pestis Angola (biovar Pestoides) ADI-0006632 astA CDS
Automated Process Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0001632 astA CDS
Automated Process Uncurated Go view Yersinia pestis B42003004 (biovar Antiqua) BAL-0004447 astA CDS
Automated Process Uncurated Go view Yersinia pestis CA88-4125 (biovar Orientalis) BAF-0001466 astA CDS
Person Uncurated Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0121834 astA CDS
Automated Process Uncurated Go view Yersinia pestis E1979001 (biovar Antiqua) BAM-0005383 astA CDS
Automated Process Uncurated Go view Yersinia pestis FV-1 (biovar Orientalis) BAK-0000605 CDS
Automated Process Uncurated Go view Yersinia pestis IP275 (biovar Orientalis) BAC-0009135 astA CDS
Person Uncurated Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0032067 astA CDS
Automated Process Uncurated Go view Yersinia pestis MG05-1020 (biovar Orientalis) BAP-0006028 astA CDS
Automated Process Uncurated Go view Yersinia pestis Pestoides F (biovar Pestoides) BAI-0001382 CDS
Automated Process Uncurated Go view Yersinia pestis UG05-0454 (biovar Antiqua) BAN-0007219 astA CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0006093 astA CDS
Person Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0002040 astA CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0002385 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0002640 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0012260 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0004174 astA CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)

Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21964mutantTn5 InsertionRequest the StrainABE-0005822astAb1747::Tn5KAN-2 at position 315 in Plus orientation ,+ pKD460Blattner Laboratory
FB23508mutantTn5 InsertionRequest the StrainABE-0005822astAb1747::Tn5KAN-2 at position 315 in Plus orientation ,- pKD46 RESEQD By acciden5Tim Durfee (durf@genome.wisc.edu)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1824731 to 1835765
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0005809vesCDScomplement18243621824937Downstream
ABE-0005812spyCDScomplement18251401825625Downstream
ABE-0005815astECDScomplement18259551826923Downstream
ABE-0005817astBCDScomplement18269161828259Downstream
ABE-0005820astDCDScomplement18282561829734Overlaps Downstream
ABE-0005822astACDScomplement18297311830765Matches
ABE-0005824astCCDScomplement18307621831982Overlaps Upstream
ABE-0005829xthACDSforward18324281833234Upstream
ABE-0005832ydjXCDSforward18334011834111Upstream
ABE-0005834ydjYCDSforward18341161834793Upstream
ABE-0005837ydjZCDSforward18348081835515Upstream
ABE-0005839ynjACDSforward18355151836063Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.