Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0006318 (otsA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0006318 showshow history
Names
otsA pexA JW5312 ECK1895 b1896
Product
trehalose-6-phosphate synthase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 1980188 1981612 1425 475
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001830 (IPR001830 Glycosyl transferase, family 20) Apr 2008
Automated Process Approved IPR012766 (IPR012766 Alpha,alpha-trehalose-phosphate synthase) Apr 2008
Automated Process Approved PF00982 (PF00982 Glycosyltransferase family 20) Jul 2018
Automated Process Uncurated GT20 (GT20 Glycosyl Transferase Family 20) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0006006 glucose metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0005992 trehalose biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001830
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0006970 response to osmotic stress Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0003825 alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR012766
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001830
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name otsA Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK1895 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym pexA Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym JW5312 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1896 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product trehalose-6-phosphate synthase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name trehalose-6-phosphate synthase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR012766 Alpha,alpha-trehalose-phosphate synthase Protein Sequence Similarity - InterPro Domain: IPR012766
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR001830 Glycosyl transferase, family 20 Protein Sequence Similarity - InterPro Domain: IPR001830
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00982 Glycosyltransferase family 20 Protein Sequence Similarity - PFAM Model Name: PF00982
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated protein family GT20 Glycosyl Transferase Family 20 Protein Sequence Similarity - CAZy Model Name: GT20
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 2.4.1.15 Published Annotation - Swiss-Prot Accession Number: P31677 Feb 2006
Person Uncurated MultiFun 1.7.9 (metabolism; central intermediary metabolism; glucose metabolism) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 5.5.1 (cell processes; adaptation to stress; osmotic pressure) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11751 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P31677 Published Annotation - Swiss-Prot Accession Number: P31677 Jan 2005
Automated Process Approved db xref GeneID:946405 Published Annotation - Entrez Gene Database ID: 946405
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref UniProtKB/Swiss-Prot:P31677 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1788206 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0006318 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Osmotic adaptation Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note GO_process: GO:0006006 - glucose metabolic process; GO_process: GO:0006970 - response to osmotic stress Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC74966.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.50
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0006970 response to osmotic stress Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO biological process GO:0006006 glucose metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0005992 trehalose biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001830
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0003825 alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR012766
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001830
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0004200 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002644 otsA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002695 otsA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002684 otsA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002561 otsA CDS
Ortholog
Automated Process Uncurated Go view Brenneria salicis ATCC 15712 [T] AMK-0001349 CDS
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0003071 CDS
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0002572 otsA CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002684 otsA CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0003432 otsA CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0003282 otsA CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0302472 otsA CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC BAA-2158 APN-0002280 otsA CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0001516 otsA CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0008313 otsA CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0001850 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009632 otsA CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001786 otsA CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002491 otsA CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007406 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0009007 otsA CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0008419 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078429 otsA CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002121 otsA CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002459 otsA CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008383 otsA CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0009356 otsA CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0002142 otsA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006565 otsA CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000151 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0010317 otsA CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0009714 otsA CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006110 otsA CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0007473 otsA CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0009134 otsA CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0010636 otsA CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0009816 otsA CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0010299 otsA CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007888 otsA CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0010023 otsA CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026691 otsA CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0008104 otsA CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006671 otsA CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002334 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001786 otsA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0001945 otsA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0002078 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002309 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001999 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0002165 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0176328 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002161 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001288 otsA CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132588 otsA CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0003953 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0002104 otsA CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0002400 otsA CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0000649 otsA CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0001630 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0003396 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0001576 otsA CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0002486 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0002303 otsA CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0001912 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0009354 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0001550 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0285682 otsA CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0005494 otsA CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0005371 otsA CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0001052 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0002272 otsA CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0005589 otsA CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0003577 otsA CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0003500 otsA CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0004497 otsA CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006506 otsA CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0005446 otsA CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0008303 otsA CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0008265 otsA CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0005622 otsA CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0006453 otsA CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0004319 otsA CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0000939 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0000940 otsA CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0001239 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006694 otsA CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0007597 otsA CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0006166 otsA CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0109252 otsA CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0082663 otsA CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0002347 otsA CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0096236 otsA CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007376 otsA CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0007053 otsA CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0008304 otsA CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0006815 otsA CDS
Person Uncurated Go view Shigella dysenteriae 197 ADU-0001355 otsA CDS
Person Uncurated Go view Shigella flexneri 2457T ABQ-0073145 otsA pseudogene
Person Approved Go view Shigella sonnei 046 ADD-0001316 otsA CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0015387 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003352 otsA CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20502mutantTn5 InsertionRequest the StrainABE-0006318otsAotsA::Tn5KAN-2 at position 621 in Minus orientation ,+ pKD460Blattner Laboratory
FB20503mutantTn5 InsertionRequest the StrainABE-0006318otsAotsA::Tn5KAN-2 at position 621 in Minus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 1975188 to 1986612
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0006294cheACDScomplement19733601975324Downstream
ABE-0006297motBCDScomplement19753291976255Downstream
ABE-0006299motACDScomplement19762521977139Downstream
ABE-0285840motRncRNAcomplement19772081977302Downstream
ABE-0006301flhCCDScomplement19772661977844Downstream
ABE-0006303flhDCDScomplement19778471978197Downstream
ABE-0006305mobile_elementcomplement19785031979270Downstream
ABE-0006308insB-5CDScomplement19785181979021Downstream
ABE-0006311insA-5CDScomplement19789401979215Downstream
ABE-0006316uspCCDSforward19797531980181Downstream
ABE-0006318otsACDScomplement19801881981612Matches
ABE-0006320otsBCDScomplement19815871982387Overlaps Upstream
ABE-0174093araHCDScomplement19825541983540Upstream
ABE-0006328araGCDScomplement19835551985069Upstream
ABE-0006330araFCDScomplement19851391986128Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.