Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0006611 (cobT) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0006611 showshow history
Names
cobT JW1969 ECK1986 b1991
Product
nicotinate-nucleotide dimethylbenzimidazole-P phophoribosyl transferase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2063388 2064467 1080 360
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR003200 (IPR003200 Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase) Apr 2008
Automated Process Approved IPR012064 (IPR012064 (DEPRECATED) Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, bacterial) Apr 2008
Automated Process Approved IPR017846 (IPR017846 Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, bacterial type) Jul 2018
Automated Process Approved IPR036087 (IPR036087 Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase-like superfamily) Jul 2018
Automated Process Approved PF02277 (PF02277 Phosphoribosyltransferase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Approved GO biological process GO:0009236 cobalamin biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0009163 nucleoside biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR017846
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0009236 cobalamin biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012064
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0008939 nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012064
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name cobT Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW1969 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK1986 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1991 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product nicotinate-nucleotide dimethylbenzimidazole-P phophoribosyl transferase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name nicotinate-nucleotide dimethylbenzimidazole-P phophoribosyl transferase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Automated Process Approved alternate product name nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Protein Family
Automated Process Approved protein family IPR003200 Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase Protein Sequence Similarity - InterPro Domain: IPR003200
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR036087 Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase-like superfamily Protein Sequence Similarity - InterPro Domain: IPR036087
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR012064 (DEPRECATED) Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, bacterial Protein Sequence Similarity - InterPro Domain: IPR012064
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF02277 Phosphoribosyltransferase Protein Sequence Similarity - PFAM Model Name: PF02277
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR017846 Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, bacterial type Protein Sequence Similarity - InterPro Domain: IPR017846
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 2.4.2.21 Published Annotation - Swiss-Prot Accession Number: P36562 Feb 2006
Person Uncurated MultiFun 1.5.3.13 (metabolism; building block biosynthesis; cofactor, small molecule carrier; cobalamin (Vitamin B12)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P36562 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946517 Published Annotation - Entrez Gene Database ID: 946517
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P36562 Published Annotation - Swiss-Prot Accession Number: P36562 Jan 2005
Automated Process Approved db xref GI:1788300 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12151 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0006611 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Biosynthesis of cofactors, carriers: Cobalamin Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved protein id AAC75052.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0009236 cobalamin biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012064
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0009163 nucleoside biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR017846
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved GO biological process GO:0009236 cobalamin biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0008939 nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012064
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0004292 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002663 cobT CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002753 cobT CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0007604 cobT CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002530 cobT CDS
Ortholog
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0004339 CDS
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0002754 cobT CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002832 cobT CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0003508 cobT CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0004940 cobT CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002007 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009428 cobT CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001926 cobT CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002395 cobT CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007283 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0008431 cobT CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0008046 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078608 cobT CDS
Person Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002283 cobT pseudogene
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002624 cobT CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0010421 cobT CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0006318 cobT CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0002262 cobT CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006710 cobT CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0005999 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0006236 cobT CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0010518 cobT CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0010598 cobT CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0007676 cobT CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0009704 cobT CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0010430 cobT CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0010319 cobT CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0009734 cobT CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009616 cobT CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0009692 cobT CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026876 cobT CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0004776 cobT CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006789 cobT CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002490 cobT CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001867 cobT CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002043 cobT CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001982 cobT CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002394 cobT CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001891 cobT CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0002322 cobT CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0176457 cobT CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002315 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001132 cobT CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132786 cobT CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004683 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0002236 cobT CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003191 cobT CDS
Person Uncurated Go view Photorhabdus luminescens subsp. laumondii TTO1 [T] ACD-0143681 cobT CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006307 cobT CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0000908 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0002385 cobT CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006618 cobT CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0006426 cobT CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0006107 cobT CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0004403 cobT CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006600 cobT CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0005900 cobT CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0006345 cobT CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0006555 cobT CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0007718 cobT CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0000856 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0000855 cobT CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0001098 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006789 cobT CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0008267 cobT CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0006265 cobT CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007949 cobT CDS
Person Uncurated Go view Salmonella Typhi CT18 ABX-0109451 cobT CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0082571 cobT CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0002508 cobT CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0096476 cobT CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007270 cobT CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0004781 cobT CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0005288 cobT CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0264590 cobT CDS
Person Uncurated Go view Shigella boydii 227 ADV-0001335 cobT CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006363 cobT CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0002583 cobT CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0073280 cobT CDS
Person Approved Go view Shigella flexneri 301 ABT-0088346 cobT CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0002213 cobT CDS
Person Approved Go view Shigella sonnei 046 ADD-0002256 cobT CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0002039 cobT CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0000511 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0007782 cobT CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0000445 cobT CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0002886 cobT CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0001259 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0016183 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003184 cobT CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20560mutantTn5 InsertionRequest the StrainABE-0006611cobTcobT::Tn5KAN-2 at position 606 in Plus orientation ,- pKD460Blattner Laboratory
FB20561mutantTn5 InsertionRequest the StrainABE-0006611cobTcobT::Tn5KAN-2 at position 606 in Plus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2058388 to 2069467
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0006586yeeOCDScomplement20582032059657Downstream
ABE-0006588asnUtRNAforward20598512059926Downstream
ABE-0006590cblCDScomplement20599642060914Downstream
ABE-0006594nacCDScomplement20610162061933Downstream
ABE-0006605asnVtRNAforward20622602062335Downstream
ABE-0006607ldtACDScomplement20623912063323Downstream
ABE-0006611cobTCDScomplement20633882064467Matches
ABE-0006613cobSCDScomplement20644792065222Upstream
ABE-0006616cobUCDScomplement20652192065764Upstream
ABE-0285237yeeHpseudogenecomplement20660652066154(Interval #2) Upstream
ABE-0006617is_elementforward20661592067353Upstream
ABE-0234210CP4-44prophageforward20661592079031Upstream
ABE-0285478mobile_elementcomplement20661592067353Upstream
ABE-0006619insH6CDScomplement20663052067285Upstream
ABE-0285237yeeHpseudogenecomplement20673542067455(Interval #1) Upstream
ABE-0285479misc_featureforward20673542079031Upstream
ABE-0285238yoeGpseudogenecomplement20674562067892Upstream
ABE-0285239yoeHpseudogenecomplement20682522068419Upstream
ABE-0006626yoeA_1CDSpforward20686352069027Upstream
ABE-0285114yoeApseudogeneforward20686352068940(Interval #1) Upstream
ABE-0006627is_elementforward20689412070271Upstream
ABE-0285480mobile_elementcomplement20689412070271Upstream
ABE-0006629insD-3CDScomplement20689522069857Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.