Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0006613 (cobS) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0006613 showshow history
Names
cobS JW1970 ECK1987 b1992
Product
cobalamin 5'-phosphate synthase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2064479 2065222 744 248
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR003805 (IPR003805 Adenosylcobinamide-GDP ribazoletransferase) Apr 2008
Automated Process Approved PF02654 (PF02654 Cobalamin-5-phosphate synthase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0009236 cobalamin biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003805
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Approved GO biological process GO:0009236 cobalamin biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0051073 adenosylcobinamide-GDP ribazoletransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003805
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0008818 cobalamin 5'-phosphate synthase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003805
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name cobS Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW1970 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK1987 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b1992 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product cobalamin 5'-phosphate synthase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name cobalamin 5'-phosphate synthase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Automated Process Approved alternate product name cobalamin synthase Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Protein Family
Automated Process Approved protein family PF02654 Cobalamin-5-phosphate synthase Protein Sequence Similarity - PFAM Model Name: PF02654
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR003805 Adenosylcobinamide-GDP ribazoletransferase Protein Sequence Similarity - InterPro Domain: IPR003805
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Approved EC number 2.-.-.- Published Annotation - Swiss-Prot Accession Number: P36561 Feb 2006
Automated Process Uncurated EC number 2.7.8.26 Published Annotation - Database Name: EC2GO: GO:0051073
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated MultiFun 1.5.3.13 (metabolism; building block biosynthesis; cofactor, small molecule carrier; cobalamin (Vitamin B12)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0006613 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12150 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P36561 Published Annotation - Swiss-Prot Accession Number: P36561 Jan 2005
Automated Process Approved db xref GI:1788301 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P36561 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946520 Published Annotation - Entrez Gene Database ID: 946520
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved function enzyme; Biosynthesis of cofactors, carriers: Cobalamin Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved protein id AAC75053.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0009236 cobalamin biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003805
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Approved GO biological process GO:0009236 cobalamin biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0051073 adenosylcobinamide-GDP ribazoletransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003805
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0008818 cobalamin 5'-phosphate synthase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR003805
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0004294 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002687 cobS CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002810 cobS CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0007510 cobS CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002604 cobS CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0002755 cobS CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002833 cobS CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0003509 cobS CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003566 cobS CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0004941 cobS CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002008 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009427 cobS CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0001927 cobS CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002394 cobS CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007282 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0008430 cobS CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0008045 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078609 cobS CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002285 cobS CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002625 cobS CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0010422 cobS CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0006319 cobS CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0002263 cobS CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006711 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0006000 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0006237 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0010519 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0010599 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0009655 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC4115 (EHEC) BAS-0007677 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0009703 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0010429 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0010320 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0009735 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009617 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0009691 cobS CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0010440 cobS CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026877 cobS CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0004777 cobS CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006790 cobS CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002491 cobS CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001868 cobS CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002044 cobS CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0001981 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002393 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0001890 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0002323 cobS CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0176458 cobS CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002316 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0001131 cobS CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132787 cobS CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0004682 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0002237 cobS CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0002472 cobS CDS
Person Uncurated Go view Photorhabdus luminescens subsp. laumondii TTO1 [T] ACD-0143688 cobS CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0001747 cobS CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006308 cobS CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0000907 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0002386 cobS CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006619 cobS CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0006429 cobS CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0006109 cobS CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0004402 cobS CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006601 cobS CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0005901 cobS CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0006346 cobS CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0003982 cobS CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0005728 cobS CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0006556 cobS CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0007717 cobS CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0000855 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0000854 cobS CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0001097 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006790 cobS CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0008266 cobS CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0006266 cobS CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007950 cobS CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0109453 cobS CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0082570 cobS CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0002509 cobS CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0096479 cobS CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007269 cobS CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0004780 cobS CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0005289 cobS CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0264591 cobS CDS
Person Uncurated Go view Shigella boydii 227 ADV-0001334 cobS CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006362 cobS CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0003777 cobS CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0002582 cobS CDS
Person Uncurated Go view Shigella flexneri 2457T ABQ-0073281 cobS CDS
Person Approved Go view Shigella flexneri 301 ABT-0088347 cobS CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0002214 cobS CDS
Person Approved Go view Shigella sonnei 046 ADD-0002257 cobS CDS
Person Uncurated Go view Trabulsiella guamensis ATCC 49490 [T] APH-0004742 cobS CDS
Person Uncurated Go view Vibrio cholerae N16961 ADH-0001310 VC1238 CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0002037 cobS CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0000509 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0007789 cobS CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0000447 cobS CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0002884 cobS CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0001261 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0016189 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003183 cobS CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20562mutantTn5 InsertionRequest the StrainABE-0006613cobScobS::Tn5KAN-2 at position 288 in Minus orientation ,+ pKD460Blattner Laboratory
FB20563mutantTn5 InsertionRequest the StrainABE-0006613cobScobS::Tn5KAN-2 at position 288 in Minus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2059479 to 2070222
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0006586yeeOCDScomplement20582032059657Downstream
ABE-0006588asnUtRNAforward20598512059926Downstream
ABE-0006590cblCDScomplement20599642060914Downstream
ABE-0006594nacCDScomplement20610162061933Downstream
ABE-0006605asnVtRNAforward20622602062335Downstream
ABE-0006607ldtACDScomplement20623912063323Downstream
ABE-0006611cobTCDScomplement20633882064467Downstream
ABE-0006613cobSCDScomplement20644792065222Matches
ABE-0006616cobUCDScomplement20652192065764Overlaps Upstream
ABE-0285237yeeHpseudogenecomplement20660652066154(Interval #2) Upstream
ABE-0006617is_elementforward20661592067353Upstream
ABE-0234210CP4-44prophageforward20661592079031Upstream
ABE-0285478mobile_elementcomplement20661592067353Upstream
ABE-0006619insH6CDScomplement20663052067285Upstream
ABE-0285237yeeHpseudogenecomplement20673542067455(Interval #1) Upstream
ABE-0285479misc_featureforward20673542079031Upstream
ABE-0285238yoeGpseudogenecomplement20674562067892Upstream
ABE-0285239yoeHpseudogenecomplement20682522068419Upstream
ABE-0006626yoeA_1CDSpforward20686352069027Upstream
ABE-0285114yoeApseudogeneforward20686352068940(Interval #1) Upstream
ABE-0006627is_elementforward20689412070271Upstream
ABE-0285480mobile_elementcomplement20689412070271Upstream
ABE-0006629insD-3CDScomplement20689522069857Upstream
ABE-0006631insC-3CDScomplement20698152070180Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.