Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0006749 (wbbJ) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0006749 showshow history
Names
wbbJ JW2018 ECK2027 yefH b2033
Product
putative lipopolysaccharide biosynthesis O-acetyl transferase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2104494 2105084 591 197
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001451 (IPR001451 Hexapeptide repeat) Apr 2008
Automated Process Approved IPR011004 (IPR011004 Trimeric LpxA-like superfamily) Apr 2008
Automated Process Approved PF00132 (PF00132 Bacterial transferase hexapeptide (six repeats)) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Approved GO biological process GO:0009103 lipopolysaccharide biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0008415 (DEPRECATED) acyltransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001451
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name wbbJ Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym yefH Published Annotation - Database Name: GenProtEC Jul 2002
Person Approved synonym JW2018 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK2027 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2033 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product putative lipopolysaccharide biosynthesis O-acetyl transferase Published Annotation - EcoCyc Accession Number: EG11984
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Automated Process Approved alternate product name predicted lipopolysaccharide biosynthesis O-acetyl transferase Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved alternate product name predicted acyl transferase Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family IPR001451 Hexapeptide repeat Protein Sequence Similarity - InterPro Domain: IPR001451
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR011004 Trimeric LpxA-like superfamily Protein Sequence Similarity - InterPro Domain: IPR011004
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00132 Bacterial transferase hexapeptide (six repeats) Protein Sequence Similarity - PFAM Model Name: PF00132
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 2.3.1.- Published Annotation - Swiss-Prot Accession Number: P37750 Feb 2006
Person Approved MultiFun 1.6.3 (metabolism; macromolecules (cellular constituent) biosynthesis; lipopolysaccharide) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11984 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0006749 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Uncurated db xref NCBI_gi:510256 Published Annotation - GenPept Accession Number: 510256 Feb 2005
Person Approved db xref UniProtKB:P37750 Published Annotation - Swiss-Prot Accession Number: P37750 Jan 2005
Person Uncurated db xref NCBI_gi:2665493 Published Annotation - GenPept Accession Number: 2665493 Feb 2005
Automated Process Approved db xref GeneID:946556 Published Annotation - Entrez Gene Database ID: 946556
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:1788345 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P37750 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved note putative O-acetyl transferase; GO_process: GO:0009103 - lipopolysaccharide biosynthetic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75094.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Approved GO biological process GO:0009103 lipopolysaccharide biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0008415 (DEPRECATED) acyltransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001451
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002473 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0008688 wbbJ CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0009801 wbbJ CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004345 wbbJ CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0007605 wbbJ CDS
Ortholog
Person Uncurated Go view Dickeya dadantii 3937 ABF-0018565 wbbJ CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001909 wbbJ CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002090 wbbJ CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20586mutantTn5 InsertionRequest the StrainABE-0006749wbbJwbbJ::Tn5KAN-I-SceI at position 60 in Plus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2099494 to 2110084
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0006734ugdCDScomplement20984472099613Downstream
ABE-0006737gndCDScomplement20998622101268Downstream
ABE-0285061wbbL_2CDSpcomplement21013962101677Downstream
ABE-0285104wbbLpseudogenecomplement21013962101744(Interval #2) Downstream
ABE-0006740is_elementforward21017492102943Downstream
ABE-0285482mobile_elementcomplement21017492102943Downstream
ABE-0006742insH7CDScomplement21018952102875Downstream
ABE-0006744wbbL_1CDSpcomplement21029162103389Downstream
ABE-0285104wbbLpseudogenecomplement21029442103389(Interval #1) Downstream
ABE-0006747wbbKCDScomplement21033912104509Overlaps Downstream
ABE-0006749wbbJCDScomplement21044942105084Matches
ABE-0006751wbbICDScomplement21050652106057Overlaps Upstream
ABE-0006753wbbHCDScomplement21060602107226Upstream
ABE-0006755glfCDScomplement21072262108329Upstream
ABE-0006757rfbXCDScomplement21083372109584Upstream
ABE-0006759rfbCCDScomplement21095812110138Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.