Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0006755 (glf) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0006755 showshow history
Names
glf yefE JW2021 ECK2030 b2036
Product
UDP-galactopyranose mutase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2107226 2108329 1104 368
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR013027 (IPR013027 (DEPRECATED) FAD-dependent pyridine nucleotide-disulphide oxidoreductase) Apr 2008
Automated Process Approved IPR004379 (IPR004379 UDP-galactopyranose mutase) Apr 2008
Automated Process Approved IPR015899 (IPR015899 UDP-galactopyranose mutase, C-terminal) Jul 2018
Automated Process Approved PF03275 (PF03275 UDP-galactopyranose mutase) Jul 2018
Automated Process Approved PF13450 (PF13450 NAD(P)-binding Rossmann-like domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0009103 lipopolysaccharide biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004379
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0009243 O antigen biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0008767 UDP-galactopyranose mutase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004379
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO molecular function GO:0042280 (OBSOLETE) cell surface antigen activity, host-interacting Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name glf Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW2021 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym yefE Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym ECK2030 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2036 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product UDP-galactopyranose mutase Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 		Oct 2021
Person Approved alternate product name UDP-galactopyranose mutase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name UDP-galactopyranose mutase, FAD/NAD(P)-binding domain Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR013027 (DEPRECATED) FAD-dependent pyridine nucleotide-disulphide oxidoreductase Protein Sequence Similarity - InterPro Domain: IPR013027
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF13450 NAD(P)-binding Rossmann-like domain Protein Sequence Similarity - PFAM Model Name: PF13450
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR015899 UDP-galactopyranose mutase, C-terminal Protein Sequence Similarity - InterPro Domain: IPR015899
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF03275 UDP-galactopyranose mutase Protein Sequence Similarity - PFAM Model Name: PF03275
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR004379 UDP-galactopyranose mutase Protein Sequence Similarity - InterPro Domain: IPR004379
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Approved EC number 5.4.99.9 Published Annotation - Swiss-Prot Accession Number: P37747 Feb 2006
Person Uncurated MultiFun 6.2 (cell structure; peptidoglycan (murein)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.3 (cell structure; surface antigens (ECA, O antigen of LPS)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.6.3.1 (metabolism; macromolecules (cellular constituent) biosynthesis; lipopolysaccharide; O antigen) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945235 Published Annotation - Entrez Gene Database ID: 945235
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P37747 Published Annotation - Swiss-Prot Accession Number: P37747 Jan 2005
Person Uncurated db xref NCBI_gi:508242 Published Annotation - GenPept Accession Number: 508242 Feb 2005
Automated Process Approved db xref GI:1788348 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P37747 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Uncurated db xref NCBI_gi:510253 Published Annotation - GenPept Accession Number: 510253 Feb 2005
Automated Process Approved db xref EcoGene:EG11981 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0006755 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Degradation of small molecules: Carbon compounds Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note UDP-galactopyranose mutase; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009243 - O antigen biosynthetic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75097.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0009103 lipopolysaccharide biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004379
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0009243 O antigen biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0008767 UDP-galactopyranose mutase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004379
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO molecular function GO:0042280 (OBSOLETE) cell surface antigen activity, host-interacting Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Comparative to toptop
Author Approval Details Genome Feature Name Type
Homolog
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0002022 CDS
Person Uncurated Go view Salmonella Typhi CT18 ABX-0106779 pseudogene
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0083871 CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0092712 CDS
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0004013 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0005281 glf CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0005245 glf CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004860 glf CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0005795 glf CDS
Ortholog
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0302794 rfbD CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0001394 glf CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001912 glf CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002093 glf CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0006207 CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0003715 CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0004171 CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0002945 glf CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB23367mutantTn5 InsertionRequest the StrainABE-0006755glfglf::Tn5KAN-I-SceI at position 589 in Plus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2102226 to 2113329
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0006740is_elementforward21017492102943Downstream
ABE-0285482mobile_elementcomplement21017492102943Downstream
ABE-0006742insH7CDScomplement21018952102875Downstream
ABE-0006744wbbL_1CDSpcomplement21029162103389Downstream
ABE-0285104wbbLpseudogenecomplement21029442103389(Interval #1) Downstream
ABE-0006747wbbKCDScomplement21033912104509Downstream
ABE-0006749wbbJCDScomplement21044942105084Downstream
ABE-0006751wbbICDScomplement21050652106057Downstream
ABE-0006753wbbHCDScomplement21060602107226Overlaps Downstream
ABE-0006755glfCDScomplement21072262108329Matches
ABE-0006757rfbXCDScomplement21083372109584Upstream
ABE-0006759rfbCCDScomplement21095812110138Upstream
ABE-0006762rfbACDScomplement21101382111019Upstream
ABE-0006764rfbDCDScomplement21110772111976Upstream
ABE-0006766rfbBCDScomplement21119762113061Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.