Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0006757 (rfbX) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0006757 showshow history
Names
rfbX wzxB JW2022 ECK2031 wzx b2037
Product
polyisoprenol-linked O-antigen repeat unit flippase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2108337 2109584 1248 416
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR002797 (IPR002797 Polysaccharide biosynthesis protein) Apr 2008
Automated Process Approved PF01943 (PF01943 Polysaccharide biosynthesis protein) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Approved GO biological process GO:0009243 O antigen biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0000271 polysaccharide biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002797
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002797
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name rfbX Published Annotation - PubMed ID: 5231414
Reference: Salmonella enterica 		Apr 2020
Person Approved synonym wzx Published Sequence Analysis - PubMed ID: 9004408 Feb 2005
Person Approved synonym JW2022 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK2031 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym wzxB Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved locus tag b2037 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product polyisoprenol-linked O-antigen repeat unit flippase Experimental - PubMed ID: 8606190
Reference: Salmonella enterica 		Apr 2020
Person Uncurated alternate product name predicted polisoprenol-linked O-antigen transporter Published Annotation - PubMed ID: 16397293 Apr 2020
Person Approved alternate product name putative O-antigen transporter Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR002797 Polysaccharide biosynthesis protein Protein Sequence Similarity - InterPro Domain: IPR002797
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF01943 Polysaccharide biosynthesis protein Protein Sequence Similarity - PFAM Model Name: PF01943
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.9.A.1 (transport; Transporters of Unknown Classification; recognized transporters of unknown biochemical mechanism; The Polysaccharide Transporter (PST) Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.3 (cell structure; surface antigens (ECA, O antigen of LPS)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.105 (transport; substrate; lipopolysaccharide) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.6.3.1 (metabolism; macromolecules (cellular constituent) biosynthesis; lipopolysaccharide; O antigen) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0006757 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946557 Published Annotation - Entrez Gene Database ID: 946557
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Uncurated db xref NCBI_gi:510252 Published Annotation - GenPept Accession Number: 510252 Feb 2005
Person Uncurated db xref NCBI_gi:2665492 Published Annotation - GenPept Accession Number: 2665492 Feb 2005
Automated Process Approved db xref GI:1788349 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P37746 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11980 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P37746 Published Annotation - Swiss-Prot Accession Number: P37746 Jan 2005
Person Approved function putative transport; Surface polysaccharides & antigens Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note putative O-antigen transporter; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009243 - O antigen biosynthetic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75098.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0000271 polysaccharide biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002797
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Approved GO biological process GO:0009243 O antigen biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002797
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0003961 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0005467 rfbX CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0005103 rfbX CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0005664 wzxB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0005948 rfbX CDS
Ortholog
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001913 rfbX CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002094 rfbX CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20589mutantTn5 InsertionRequest the StrainABE-0006757rfbXrfbX::Tn5KAN-2 at position 1057 in Plus orientation ,+ pKD460Blattner Laboratory
FB20590mutantTn5 InsertionRequest the StrainABE-0006757rfbXrfbX::Tn5KAN-2 at position 1057 in Plus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2103337 to 2114584
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0006744wbbL_1CDSpcomplement21029162103389Downstream
ABE-0285104wbbLpseudogenecomplement21029442103389(Interval #1) Downstream
ABE-0006747wbbKCDScomplement21033912104509Downstream
ABE-0006749wbbJCDScomplement21044942105084Downstream
ABE-0006751wbbICDScomplement21050652106057Downstream
ABE-0006753wbbHCDScomplement21060602107226Downstream
ABE-0006755glfCDScomplement21072262108329Downstream
ABE-0006757rfbXCDScomplement21083372109584Matches
ABE-0006759rfbCCDScomplement21095812110138Overlaps Upstream
ABE-0006762rfbACDScomplement21101382111019Upstream
ABE-0006764rfbDCDScomplement21110772111976Upstream
ABE-0006766rfbBCDScomplement21119762113061Upstream
ABE-0006771galFCDScomplement21134342114327Upstream
ABE-0006774wcaMCDScomplement21145022115896Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.