|
|
EC number |
1.1.1.271
|
Published Annotation -
Swiss-Prot Accession Number: P32055
|
Feb 2006 |
|
|
MultiFun |
7.1
(location of gene products; cytoplasm)
|
Published Annotation -
MultiFun Database Name: MultiFun |
Feb 2002 |
|
|
MultiFun |
6.3
(cell structure; surface antigens (ECA, O antigen of LPS))
|
Published Annotation -
MultiFun Database Name: MultiFun |
Feb 2002 |
|
|
MultiFun |
6.7
(cell structure; capsule (M and K antigens))
|
Published Annotation -
MultiFun Database Name: MultiFun |
Feb 2002 |
|
|
MultiFun |
1.6.2
(metabolism; macromolecules (cellular constituent) biosynthesis; colanic acid (M antigen))
|
Published Annotation -
MultiFun Database Name: MultiFun |
Feb 2002 |
|
|
codon start |
1
|
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
|
Oct 2013 |
|
|
comment |
The epimerase and reduction reactions occur at the same active site.
|
Experimental -
PubMed ID: 9862812
Reference: Escherichia coli
|
Aug 2005 |
|
|
comment |
The gene product has two distinct activities: the epimerization at C-3 and C-5 of GDP-4-keto,6-deoxy-(D)-mannose and the reduction at C-4 of GDP-4-keto,6-deoxy-(L)-glucose.
|
Experimental -
PubMed ID: 9525924
Reference: Escherichia coli
|
Aug 2005 |
|
|
comment |
NADPH is required for the reduction reaction but not for the epimerase reaction.
|
Experimental -
PubMed ID: 10480878
Reference: Escherichia coli
|
Aug 2005 |
|
|
db xref |
ASAP:ABE-0006801
|
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
|
Oct 2013 |
|
|
db xref |
EcoGene:EG11788
|
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
|
Oct 2013 |
|
|
db xref |
GI:1788365
|
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
|
Oct 2013 |
|
|
db xref |
GeneID:946563
|
Published Annotation -
Entrez Gene Database ID: 946563
Reference: Escherichia coli K-12 MG1655
|
Jun 2009 |
|
|
db xref |
UniProtKB:P32055
|
Published Annotation -
Swiss-Prot Accession Number: P32055
|
Jan 2005 |
|
|
db xref |
NCBI_gi:1407613
|
Published Annotation -
GenPept Accession Number: AAC77843
|
Sep 2005 |
|
|
function |
resistance to acid stress
|
Experimental -
PubMed ID: 11371548
|
Nov 2005 |
|
|
function |
resistance to desiccation
|
Experimental -
PubMed Central ID: 201377
Reference: Escherichia coli
|
Sep 2005 |
|
|
function |
resistance to thermal stress
|
Experimental -
PubMed ID: 11371548
|
Nov 2005 |
|
|
function |
colanic acid biosynthesis (M antigen)
|
Published Sequence Analysis -
PubMed ID: 8759852
|
Nov 2005 |
|
|
function |
second and third of three steps in the biosynthetic pathway from GDP-(D)-mannose to GDP-(L)-fucose
|
Experimental -
PubMed ID: 9473059
Reference: Escherichia coli
|
Aug 2005 |
|
|
function |
biosynthesis of L-fucose component of colanic acid (M antigen)
|
Published Sequence Analysis -
PubMed ID: 8759852
|
Aug 2005 |
|
|
function |
converts GDP-4-keto,6-deoxy-(D)-mannose to GDP-(L)-fucose via a GDP-4-keto,6-deoxy-(L)-glucose intermediate
|
Experimental -
PubMed ID: 9473059
Reference: Escherichia coli
|
Aug 2005 |
|
|
note |
GO_component: GO:0005737 - cytoplasm; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009242 - colanic acid biosynthetic process; GO_process: GO:0009269 - response to desiccation
|
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
|
Oct 2013 |
|
|
physical properties |
Vmax: 363.5 +/- 21.2 micromol/min/mg; for NADPH and GDP-4-keto,6-deoxy-(D)-mannose
|
Experimental -
PubMed ID: 10480878
Reference: Escherichia coli
|
Aug 2005 |
|
|
physical properties |
Km: 38.6 +/- 4.4 microM; for GDP-4-keto,6-deoxy-(D)-mannose
|
Experimental -
PubMed ID: 11021971
Reference: Escherichia coli
|
Aug 2005 |
|
|
physical properties |
Km: 14.1 +/- 1.3 microM; for NADPH
|
Experimental -
PubMed ID: 11021971
Reference: Escherichia coli
|
Aug 2005 |
|
|
physical properties |
Vmax: 12.32 +/- 0.27 micrmol/min/mg; for NADPH and GDP-4-keto,6-deoxy-(D)-mannose
|
Experimental -
PubMed ID: 11021971
Reference: Escherichia coli
|
Aug 2005 |
|
|
physical properties |
Km: 29 +/- 1.4 microM; for GDP-4-keto,6-deoxy-(D)-mannose
|
Experimental -
PubMed ID: 10480878
|
Aug 2005 |
|
|
physical properties |
Km: 9 +/- 0.8 microM; for NADPH
|
Experimental -
PubMed ID: 10480878
|
Aug 2005 |
|
|
physical properties |
Molecular Weight:70 kD; for homodimer; by SDS-PAGE
|
Experimental -
PubMed ID: 9761875
Reference: Escherichia coli
|
Aug 2005 |
|
|
protein id |
AAC75113.1
|
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
|
Oct 2013 |
|
|
structure |
PDB:1BSV, wt, whole protein, co-crystallized with NADPH:2.20 A; X-ray diffraction.
|
Experimental -
PubMed ID: 9862812
|
Aug 2005 |
|
|
structure |
PDB:1E7Q, mutant; S107A, whole protein, co-crystallized with NADP:1.6 A; X-ray diffraction.
|
Experimental -
PubMed ID: 11021971
|
Aug 2005 |
|
|
structure |
PDB:1BWS, wt, whole protein, co-crystallized with NADPH;2.2A; X-ray diffraction.
|
Experimental -
PubMed ID: 9817848
Reference: Escherichia coli
|
Aug 2005 |
|
|
structure |
PDB:1E7S, mutant; K104R, whole protein, co-crystallized with NADP:1.5 A; X-ray diffraction.
|
Experimental -
PubMed ID: 11021971
|
Aug 2005 |
|
|
structure |
PDB:1E7R, mutant; Y136E, whole protein, co-crystallized with NADP:1.6 A; X-ray diffraction.
|
Experimental -
PubMed ID: 11021971
|
Aug 2005 |
|
|
structure |
PDB:1E6U, wt, whole protein, co-crystallized with NADP:1.45 A; X-ray diffraction.
|
Experimental -
PubMed ID: 11021971
|
Aug 2005 |
|
|
structure |
PDB:1FXS, wt, whole protein, co-crystallized with NADP:2.30 A; X-ray diffraction.
|
Experimental -
PubMed ID: 9862812
|
Aug 2005 |
|
|
structure |
PDB:1GFS, wt, whole protein, pure:2.20 A; X-ray diffraction.
|
Experimental -
PubMed ID: 9862812
|
Aug 2005 |
|
|
subcellular localization |
Cytoplasmic
|
Predicted by PSORTb -
PSORTb Final_Score: 8.96 Reference: Escherichia coli K-12 MG1655
|
Feb 2008 |
|
|
subsystem |
component of in silico model iAF1260
|
Experimental -
PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655
|
Jan 2009 |
|
|
subsystem |
component of in silico model iJR904
|
Experimental -
PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655
|
Feb 2009 |
|
|
subsystem |
component of in silico model iMC1010
|
Experimental -
PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655
|
Mar 2009 |
|
|
transl table |
11
|
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
|
Oct 2013 |