Escherichia coli K-12 MG1655
About ASAP Data Release Policy University of Wisconsin—Madison
Home    Overview    Annotations    BLAST    Downloads    Comparative Genomics    Experiments    Strains    Help (this script)    User: guest  / Log On   
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0006960 (thiM) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0006960 showshow history
Names
thiM JW2091 ECK2097 b2104
Product
hydoxyethylthiazole kinase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2184513 2185301 789 263
Sequence

Get full DNA or protein sequence

View sequence with flanking regions: bases upstream and bases downstream

Context

Browse sequence in GaPP

You last hovered over feature (none)
Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000417 (IPR000417 Hydroxyethylthiazole kinase) Apr 2008
Automated Process Approved IPR011144 (IPR011144 (DEPRECATED) Hydroxyethylthiazole kinase, monofunctional) Apr 2008
Automated Process Approved IPR029056 (IPR029056 Ribokinase-like) Jul 2018
Automated Process Approved PF02110 (PF02110 Hydroxyethylthiazole kinase family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Approved GO biological process GO:0009228 thiamine biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0009228 thiamine biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000417
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0004417 hydroxyethylthiazole kinase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000417
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name thiM Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW2091 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK2097 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2104 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product hydoxyethylthiazole kinase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name hydoxyethylthiazole kinase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR000417 Hydroxyethylthiazole kinase Protein Sequence Similarity - InterPro Domain: IPR000417
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR029056 Ribokinase-like Protein Sequence Similarity - InterPro Domain: IPR029056
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF02110 Hydroxyethylthiazole kinase family Protein Sequence Similarity - PFAM Model Name: PF02110
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR011144 (DEPRECATED) Hydroxyethylthiazole kinase, monofunctional Protein Sequence Similarity - InterPro Domain: IPR011144
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Approved EC number 2.7.1.50 Published Annotation - Swiss-Prot Accession Number: P76423 Feb 2006
Person Uncurated MultiFun 1.5.3.8 (metabolism; building block biosynthesis; cofactor, small molecule carrier; thiamin) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0006960 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945142 Published Annotation - Entrez Gene Database ID: 945142
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:1788421 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P76423 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG14069 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P76423 Published Annotation - Swiss-Prot Accession Number: P76423 Jan 2005
Person Approved function enzyme; Biosynthesis of cofactors, carriers: Thiamin Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note GO_process: GO:0009228 - thiamin biosynthetic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75165.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0009228 thiamine biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000417
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Approved GO biological process GO:0009228 thiamine biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0004417 hydroxyethylthiazole kinase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000417
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0001890 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002295 thiM CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002194 thiM CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002161 thiM CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002038 thiM CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0002908 thiM CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001194 thiM CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002956 thiM CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0005797 CDS
Person Uncurated Go view Dickeya dadantii 3937 ABF-0018796 thiM CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0003065 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0003566 thiM CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0302924 thiM CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC BAA-2158 APN-0002455 thiM CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003463 thiM CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005060 thiM CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002160 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009123 thiM CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002060 thiM CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002223 thiM CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007180 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0008546 thiM CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0008342 thiM pseudogene
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078766 thiM CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002430 thiM CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002767 thiM CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0007721 thiM CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0006502 thiM CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0002392 thiM CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006829 thiM CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0005795 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005010 thiM CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0010388 thiM CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0008238 thiM CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0007923 thiM CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0008541 thiM CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0005770 thiM CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0008306 thiM CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0009499 thiM CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007429 thiM CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0006711 thiM CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0009339 thiM CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0026993 thiM CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0004927 thiM CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006913 thiM CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002681 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0001977 thiM CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002167 thiM CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0002584 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002520 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002478 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0002464 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0176614 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002413 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0000959 thiM CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0132912 thiM CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0001902 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0002385 thiM CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0002559 thiM CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0000888 thiM CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0007584 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0001900 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0002017 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0001852 thiM CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0002182 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0002597 thiM CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0001549 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0010360 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0001284 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0289568 thiM CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0005770 thiM CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0063485 thiM CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0001851 thiM CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0000822 thiM CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0000462 thiM CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0008058 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006459 thiM CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0000784 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0002530 thiM CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006758 thiM CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0006823 thiM CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0006490 thiM CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0004244 thiM CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006741 thiM CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006041 thiM CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0006496 thiM CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0004139 thiM CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0005888 thiM CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0006697 thiM CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0007566 thiM CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0000705 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0000705 thiM CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0000883 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006930 thiM CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0006378 thiM CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0006462 thiM CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0005685 thiM CDS
Person Uncurated Go view Salmonella Typhi CT18 ABX-0109812 CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0082422 CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0002652 thiM CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0096858 thiM CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007133 thiM CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0004638 thiM CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0005429 thiM CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0002119 thiM CDS
Person Uncurated Go view Shigella boydii 227 ADV-0001003 thiM CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006531 thiM CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0003632 thiM CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0002622 thiM CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0073414 thiM CDS
Person Approved Go view Shigella flexneri 301 ABT-0088443 thiM CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0002316 thiM CDS
Person Approved Go view Shigella sonnei 046 ADD-0002358 thiM CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0010329 thiM CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0002060 thiM CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0002775 thiM CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0016576 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003101 thiM CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22834mutantTn5 InsertionRequest the StrainABE-0006960thiMthiM::Tn5(KAN-I-SceI) at position 668 in Minus orientation ,+ pKD46 0Blattner Laboratory
FB23385mutantTn5 InsertionRequest the StrainABE-0006960thiMthiM::Tn5KAN-I-SceI at position 668 in Minus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2179513 to 2190301
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0006946yegTCDSforward21788212180098Downstream
ABE-0006948yegUCDSforward21800952181099Downstream
ABE-0006950yegVCDSforward21810962182061Downstream
ABE-0006952yegWCDScomplement21820352182781Downstream
ABE-0006955yegXCDScomplement21828332183651Downstream
ABE-0006958thiDCDScomplement21837162184516Overlaps Downstream
ABE-0006960thiMCDScomplement21845132185301Matches
ABE-0006964rcnRCDScomplement21855242185796Upstream
ABE-0006968rcnACDSforward21859172186741Upstream
ABE-0006970rcnBCDSforward21869602187298Upstream
ABE-0006972yehACDScomplement21873802188414Upstream
ABE-0006974yehBCDScomplement21884302190910Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.