Escherichia coli K-12 MG1655
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0007038 (yehY) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0007038 showshow history
Names
yehY JW2118 ECK2123 b2130
Product
membrane component of an ABC superfamily glycine betaine transporter
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2217400 2218557 1158 386
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000515 (IPR000515 ABC transporter type 1, transmembrane domain MetI-like) Apr 2008
Automated Process Approved IPR035906 (IPR035906 MetI-like superfamily) Jul 2018
Automated Process Approved PF00528 (PF00528 Binding-protein-dependent transport system inner membrane component) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000515
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655
Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name yehY Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Person Approved synonym JW2118 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK2123 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2130 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product membrane component of an ABC superfamily glycine betaine transporter Published Annotation - EcoCyc Accession Number: EG12011
Reference: Escherichia coli K-12 MG1655
Jan 2021
Person Approved alternate product name putative transport system permease protein Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Jan 2006
Person Uncurated alternate product name putative glycine/betaine/choline transport protein, osmoprotection (ABC superfamily, membrane) Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name predicted transporter subunit: membrane component of ABC superfamily Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family PF00528 Binding-protein-dependent transport system inner membrane component Protein Sequence Similarity - PFAM Model Name: PF00528
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR000515 ABC transporter type 1, transmembrane domain MetI-like Protein Sequence Similarity - InterPro Domain: IPR000515
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved protein family IPR035906 MetI-like superfamily Protein Sequence Similarity - InterPro Domain: IPR035906
Reference: Escherichia coli K-12 MG1655
Jul 2018
Other
Person Uncurated MultiFun 4.3.A.1.m (transport; Primary Active Transporters; Pyrophosphate Bond (ATP, GTP, P2) Hydrolysis-driven Active Transporters; The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases; ABC superfamily, membrane component) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.79 (transport; substrate; glycine betaine choline transport) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GeneID:946660 Published Annotation - Entrez Gene Database ID: 946660
Reference: Escherichia coli K-12 MG1655
Jun 2009
Person Approved db xref UniProtKB:P33361 Published Annotation - Swiss-Prot Accession Number: P33361 Jan 2005
Automated Process Approved db xref GI:1788451 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P33361 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref ASAP:ABE-0007038 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref EcoGene:EG12011 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved function putative transport; Not classified Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Automated Process Approved note putative transport system permease protein; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved protein id AAC75191.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655
Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655
Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655
Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000515
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655
Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0005413 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002185 yehY CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002313 yehY CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002278 yehY CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002431 yehY CDS
Ortholog
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0002881 CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0002972 yehY CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0003591 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003441 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005085 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002187 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009210 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002083 yehY CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002197 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007145 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0006557 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005489 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078796 yehY CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002463 yehY CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002792 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0009136 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0005747 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0002422 yehY CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006855 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000484 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005119 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0010209 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0008611 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008095 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0009859 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0005880 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0009594 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0010955 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009765 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0008893 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0009211 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027099 yehY CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0004955 yehY CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006939 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002707 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002002 yehY CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002196 yehY CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0002610 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002554 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002505 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0002489 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0176640 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002438 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0000913 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133025 yehY CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0000271 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0002410 yehY CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0000882 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0002574 yehY CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0000905 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0000563 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0001148 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0000591 yehY CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0003617 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0001304 yehY CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0002968 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0008972 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0002979 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0288107 yehY CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0004400 yehY CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006478 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0000766 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0002548 yehY CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006777 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0006889 yehY CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0006553 yehY CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0004225 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006760 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006060 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0006516 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0004158 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0005907 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0006716 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0007547 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0000687 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0000687 yehY CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0000862 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0006949 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0006397 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0006481 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0005704 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0109863 yehY CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0082404 yehY CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0002672 yehY CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0096910 yehY CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007114 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0004620 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0005448 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0004372 CDS
Person Approved Go view Shigella boydii 227 ADV-0001108 yehY CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006240 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0003586 CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0073470 yehY CDS
Person Approved Go view Shigella flexneri 301 ABT-0088484 yehY CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0002371 yehY CDS
Person Approved Go view Shigella sonnei 046 ADD-0002397 yehY CDS
Person Uncurated Go view Trabulsiella guamensis ATCC 49490 [T] APH-0001184 yehY CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0001098 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0008262 CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0002285 yehY CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0002939 yehY CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0002333 CDS
Person Uncurated Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0236423 proW2 CDS
Automated Process Uncurated Go view Yersinia pestis Angola (biovar Pestoides) ADI-0005439 CDS
Automated Process Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0001105 CDS
Automated Process Uncurated Go view Yersinia pestis B42003004 (biovar Antiqua) BAL-0005293 CDS
Automated Process Uncurated Go view Yersinia pestis CA88-4125 (biovar Orientalis) BAF-0002174 CDS
Person Uncurated Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0119201 CDS
Automated Process Uncurated Go view Yersinia pestis E1979001 (biovar Antiqua) BAM-0006049 CDS
Automated Process Uncurated Go view Yersinia pestis F1991016 (biovar Orientalis) BAQ-0004741 CDS
Automated Process Uncurated Go view Yersinia pestis FV-1 (biovar Orientalis) BAK-0001913 CDS
Automated Process Uncurated Go view Yersinia pestis IP275 (biovar Orientalis) BAC-0007705 CDS
Automated Process Uncurated Go view Yersinia pestis K1973002 (biovar Mediaevalis) BAO-0005293 CDS
Person Uncurated Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0032738 CDS
Automated Process Uncurated Go view Yersinia pestis MG05-1020 (biovar Orientalis) BAP-0005444 CDS
Automated Process Uncurated Go view Yersinia pestis Nepal516 (biovar Antiqua) BAD-0003343 CDS
Automated Process Uncurated Go view Yersinia pestis Pestoides F (biovar Pestoides) BAI-0003003 CDS
Automated Process Uncurated Go view Yersinia pestis UG05-0454 (biovar Antiqua) BAN-0006494 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0006775 CDS
Person Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0001305 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0001566 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0003403 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0016671 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)

Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2212400 to 2223557
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0007023btsRCDScomplement22122432212962Downstream
ABE-0007027btsSCDScomplement22129592214644Downstream
ABE-0007030mlrACDSforward22148662215597Downstream
ABE-0285063yohOCDSforward22156572215764Downstream
ABE-0007032yehWCDScomplement22157452216476Downstream
ABE-0007034yehXCDScomplement22164812217407Overlaps Downstream
ABE-0007038yehYCDScomplement22174002218557Matches
ABE-0007040osmFCDScomplement22185642219481Upstream
ABE-0285492repeat_regionforward22196252219658Upstream
ABE-0007043bglXCDScomplement22196922221989Upstream
ABE-0007048dldCDSforward22221852223900Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.