Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0007168 (psuK) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0007168 showshow history
Names
psuK pscK JW2153 ECK2159 yeiC b2166
Product
pseudouridine kinase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2258355 2259296 942 314
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR011611 (IPR011611 Carbohydrate kinase PfkB) Apr 2008
Automated Process Approved IPR002173 (IPR002173 Carbohydrate/purine kinase, PfkB, conserved site) Apr 2008
Automated Process Approved IPR029056 (IPR029056 Ribokinase-like) Jul 2018
Automated Process Approved PF00294 (PF00294 pfkB family carbohydrate kinase) Jul 2018
Automated Process Uncurated GH28 (GH28 Glycoside Hydrolase Family 28) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Uncurated name psuK Published Annotation in EcoGene - EG Number: EG11646
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Approved synonym JW2153 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK2159 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym yeiC Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved synonym pscK Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved locus tag b2166 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product pseudouridine kinase Experimental - PubMed ID: 18591240
Reference: Escherichia coli K-12 		Mar 2009
Protein Family
Automated Process Approved protein family IPR002173 Carbohydrate/purine kinase, PfkB, conserved site Protein Sequence Similarity - InterPro Domain: IPR002173
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR011611 Carbohydrate kinase PfkB Protein Sequence Similarity - InterPro Domain: IPR011611
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR029056 Ribokinase-like Protein Sequence Similarity - InterPro Domain: IPR029056
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00294 pfkB family carbohydrate kinase Protein Sequence Similarity - PFAM Model Name: PF00294
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated protein family GH28 Glycoside Hydrolase Family 28 Protein Sequence Similarity - CAZy Model Name: GH28
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated EC number 2.7.1.- Protein Sequence Similarity - InterPro Domain: IPR011611
Reference: Escherichia coli K-12 MG1655 		Dec 2008
Person Uncurated MultiFun 1.7.1 (metabolism; central intermediary metabolism; unassigned reversible reactions) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11646 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0007168 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P30235 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1788491 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946664 Published Annotation - Entrez Gene Database ID: 946664
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P30235 Published Annotation - Swiss-Prot Accession Number: P30235 Jan 2005
Automated Process Approved function enzyme; pseudouridine utilization Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved note putative kinase Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75227.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.26
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0005508 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003730 psuK CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0004104 psuK CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004566 psuK CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003355 psuK CDS
Ortholog
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0003010 psuK_2 CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0003729 psuK_2 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003401 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005122 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002224 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009172 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002115 yeiC CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002160 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007101 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0006593 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005452 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078837 psuK CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002501 psuK CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002828 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0009784 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0005783 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0002457 yeiC CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006899 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000528 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0006638 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005165 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0006606 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0010722 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008142 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0005791 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0005925 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0010751 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007210 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009380 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0010570 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0009256 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027143 yeiC CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0004998 psuK_1 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0006976 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002742 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002037 yeiC CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002232 yeiC CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0002653 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002597 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002548 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0176677 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002474 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0002312 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133066 yeiC CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0000227 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0002447 yeiC CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0073510 yeiC CDS
Person Approved Go view Shigella flexneri 301 ABT-0088516 yeiC CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0002406 yeiC CDS
Person Approved Go view Shigella sonnei 046 ADD-0002432 yeiC CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0003703 yeiC CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0001920 rbsK_2 CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0001832 rbsK_1 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2253355 to 2264296
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0007152nupXCDScomplement22528952254145Downstream
ABE-0007156rihBCDScomplement22542452255186Downstream
ABE-0007160yeiLCDSforward22553552256014Downstream
ABE-0285498repeat_regionforward22560382256074Downstream
ABE-0007163psuTCDScomplement22560852257335Downstream
ABE-0007165psuGCDScomplement22574292258367Overlaps Downstream
ABE-0007168psuKCDScomplement22583552259296Matches
ABE-0007172fruACDScomplement22597192261410Upstream
ABE-0007174fruKCDScomplement22614272262365Upstream
ABE-0007180fruBCDScomplement22623652263495Upstream
ABE-0007184setBCDSforward22638632265044Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.