Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0007283 (napB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0007283 showshow history
Names
napB yejY JW5367 ECK2195 b2203
Product
periplasmic nitrate reductase cytochrome c550 protein
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2298269 2298718 450 150
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR005591 (IPR005591 Nitrate reductase cytochrome c-type subunit NapB) Apr 2008
Automated Process Approved IPR011031 (IPR011031 Multihaem cytochrome) Apr 2008
Automated Process Approved IPR036280 (IPR036280 Multiheme cytochrome superfamily) Jul 2018
Automated Process Approved PF03892 (PF03892 Nitrate reductase cytochrome c-type subunit (NapB)) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO biological process GO:0017004 cytochrome complex assembly Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0030288 outer membrane-bounded periplasmic space GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0042597 periplasmic space Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO molecular function GO:0009054 (DEPRECATED) electron acceptor activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name napB Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK2195 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW5367 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym yejY Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved locus tag b2203 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product periplasmic nitrate reductase cytochrome c550 protein Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 		Oct 2021
Person Uncurated alternate product name periplasmic nitrate reductase, small subunit, cytochrome C550, in complex with NapA Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name cytochrome c-type protein Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR011031 Multihaem cytochrome Protein Sequence Similarity - InterPro Domain: IPR011031
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR005591 Nitrate reductase cytochrome c-type subunit NapB Protein Sequence Similarity - InterPro Domain: IPR005591
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR036280 Multiheme cytochrome superfamily Protein Sequence Similarity - InterPro Domain: IPR036280
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF03892 Nitrate reductase cytochrome c-type subunit (NapB) Protein Sequence Similarity - PFAM Model Name: PF03892
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated MultiFun 1.6.15.1 (metabolism; macromolecules (cellular constituent) biosynthesis; large molecule carriers; cytochromes) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.2 (location of gene products; periplasmic space) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.4.2 (metabolism; energy production/transport; electron acceptor) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12061 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:87082065 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946698 Published Annotation - Entrez Gene Database ID: 946698
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0ABL3 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0007283 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P0ABL3 Published Annotation - Swiss-Prot Accession Number: P0ABL3 Jan 2006
Person Approved function carrier; Energy metabolism, carbon: Electron transport Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note cytochrome c-type protein; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0017004 - cytochrome complex assembly Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75263.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Periplasmic Predicted by PSORTb - PSORTb Final_Score: 9.76
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO biological process GO:0017004 cytochrome complex assembly Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO cellular component GO:0042597 periplasmic space Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0030288 outer membrane-bounded periplasmic space GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO molecular function GO:0009054 (DEPRECATED) electron acceptor activity Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0003206 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002296 napB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002535 napB CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0003716 napB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002583 napB CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0003201 napB CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0000882 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0003047 napB CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0003780 napB CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003341 napB CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005163 napB CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002262 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0009102 napB CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002147 napB CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002122 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0007054 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0006635 napB CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005412 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078878 napB CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002557 napB CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002868 napB CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0006488 napB CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0005825 napB CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0002493 napB CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006937 napB CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000566 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0006677 napB CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005203 napB CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0006645 napB CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007721 napB CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008180 napB CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0005752 napB CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0005965 napB CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0006900 napB CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007249 napB CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0010256 napB CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0010127 napB CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0007422 napB CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027178 napB CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0005039 napB CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007014 napB CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002776 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002071 napB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002269 napB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0002751 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002654 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002587 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0002580 napB CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0176720 napB CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002512 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0002350 napB CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133101 napB CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0000189 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0002488 napB CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0003692 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0010602 CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0062152 napB CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0003995 napB CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0001686 napB CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0002128 napB CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0007380 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006560 napB CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0000662 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0002644 napB CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006864 napB CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0007192 napB CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0006836 napB CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0006275 napB CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006858 napB CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006159 napB CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0006596 napB CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0004239 napB CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0005989 napB CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0006803 napB CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0007460 napB CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0000609 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0000608 napB CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0000742 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0007045 napB CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0005139 napB CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0006561 napB CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0005785 napB CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0110095 napB CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0082321 napB CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0002789 napB CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0097183 napB CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0007026 napB CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0004531 napB CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006794 napB CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0264400 napB CDS
Person Approved Go view Shigella boydii 227 ADV-0002338 napB CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0006135 napB CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0006946 napB CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0000988 napB CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0073550 napB CDS
Person Approved Go view Shigella flexneri 301 ABT-0088553 napB CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0002445 napB CDS
Person Approved Go view Shigella sonnei 046 ADD-0002474 napB CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0000824 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0003458 napB CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0001467 napB CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0003172 napB CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0002878 CDS
Person Uncurated Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0240724 napB CDS
Automated Process Uncurated Go view Yersinia pestis Angola (biovar Pestoides) ADI-0006904 napB CDS
Automated Process Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0002689 napB CDS
Automated Process Uncurated Go view Yersinia pestis B42003004 (biovar Antiqua) BAL-0006288 napB CDS
Automated Process Uncurated Go view Yersinia pestis CA88-4125 (biovar Orientalis) BAF-0000476 napB CDS
Person Uncurated Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0125485 napB CDS
Automated Process Uncurated Go view Yersinia pestis E1979001 (biovar Antiqua) BAM-0007614 napB CDS
Automated Process Uncurated Go view Yersinia pestis F1991016 (biovar Orientalis) BAQ-0007078 napB CDS
Automated Process Uncurated Go view Yersinia pestis IP275 (biovar Orientalis) BAC-0004996 napB CDS
Automated Process Uncurated Go view Yersinia pestis K1973002 (biovar Mediaevalis) BAO-0005689 napB CDS
Person Uncurated Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0031123 napB CDS
Automated Process Uncurated Go view Yersinia pestis MG05-1020 (biovar Orientalis) BAP-0008730 napB CDS
Automated Process Uncurated Go view Yersinia pestis Nepal516 (biovar Antiqua) BAD-0001622 napB CDS
Automated Process Uncurated Go view Yersinia pestis Pestoides F (biovar Pestoides) BAI-0002570 CDS
Automated Process Uncurated Go view Yersinia pestis UG05-0454 (biovar Antiqua) BAN-0005574 napB CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0005233 napB CDS
Person Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0002854 napB CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0003424 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0001618 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20696mutantTn5 InsertionRequest the StrainABE-0007283napBnapB::Tn5KAN-2 at position 208 in Minus orientation ,+ pKD460Blattner Laboratory
FB20697mutantTn5 InsertionRequest the StrainABE-0007283napBnapB::Tn5KAN-2 at position 208 in Minus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2293269 to 2303718
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0007269ccmFCDScomplement22929612294904Downstream
ABE-0007271ccmECDScomplement22949012295380Downstream
ABE-0007273ccmDCDScomplement22953772295586Downstream
ABE-0007275ccmCCDScomplement22955832296320Downstream
ABE-0007277ccmBCDScomplement22963622297024Downstream
ABE-0007279ccmACDScomplement22970212297644Downstream
ABE-0007281napCCDScomplement22976572298259Downstream
ABE-0007283napBCDScomplement22982692298718Matches
ABE-0007285napHCDScomplement22987152299578Overlaps Upstream
ABE-0007287napGCDScomplement22995652300260Upstream
ABE-0007289napACDScomplement23002672302753Upstream
ABE-0007291napDCDScomplement23027502303013Upstream
ABE-0007293napFCDScomplement23030032303497Upstream
ABE-0285187yojOCDSforward23036062303770Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.