Escherichia coli K-12 MG1655
About ASAP Data Release Policy University of Wisconsin—Madison
Home    Overview    Annotations    BLAST    Downloads    Comparative Genomics    Experiments    Strains    Help (this script)    User: guest  / Log On   
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0007466 (arnF) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0007466 showshow history
Names
arnF pbgE3 pmrM JW5373 ECK2252 yfbJ b2258
Product
undecaprenyl phosphate-alpha-L-Ara4N (4-amino-4-deoxy-L-arabinose) flippase component
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 2372892 2373278 387 129
Sequence

Get full DNA or protein sequence

View sequence with flanking regions: bases upstream and bases downstream

Context

Browse sequence in GaPP

You last hovered over feature (none)
Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000390 (IPR000390 Small multidrug resistance protein family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000390
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0005887 integral component of plasma membrane GO Inferred from Sequence or Structural Similarity - PubMed ID: 17928292
Reference: Escherichia coli K-12 W3110 		Dec 2007
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Uncurated name arnF Published Annotation - PubMed ID: 17928292
Reference: Escherichia coli K-12 W3110 		Dec 2007
Automated Process Approved synonym pbgE3 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved synonym pmrM Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym ECK2252 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW5373 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym yfbJ Published Annotation - Database Name: GenProtEC Jul 2002
Person Approved locus tag b2258 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product undecaprenyl phosphate-alpha-L-Ara4N (4-amino-4-deoxy-L-arabinose) flippase component Published Annotation - PubMed ID: 17928292
Reference: Escherichia coli K-12 W3110 		Dec 2007
Person Uncurated alternate product name predicted inner membrane protein Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 		Dec 2007
Automated Process Approved alternate product name undecaprenyl phosphate-alpha-L-ara4N exporter; flippase ArnEF subunit Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved alternate product name putative transport/receptor protein Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR000390 Small multidrug resistance protein family Protein Sequence Similarity - InterPro Domain: IPR000390
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG14094 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:87082075 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:945344 Published Annotation - Entrez Gene Database ID: 945344
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P76474 Published Annotation - Swiss-Prot Accession Number: P76474 Jan 2006
Automated Process Approved db xref ASAP:ABE-0007466 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P76474 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Uncurated function flips undecaprenyl phosphate-alpha-L-Ara4N in the inner membrane, exposing the sugar subunit to the periplasmic space Published Annotation - PubMed ID: 17928292
Reference: Escherichia coli K-12 W3110 		Dec 2007
Automated Process Approved function transport; Transport of small molecules: lipids Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Uncurated molecular interaction predicted to form heterodimer with arnE Published Annotation - PubMed ID: 17928292
Reference: Escherichia coli K-12 W3110 		Dec 2007
Person Uncurated mutant phenotype arnF null mutant: gene replacement with kan; an arnF null mutant has reduced levels of L-Ara4N modified lipid A Experimental - PubMed ID: 17928292
Reference: Escherichia coli K-12 W3110 		Dec 2007
Person Uncurated mutant phenotype arnF null mutant: gene replacement with kan; an arnF null mutant is sensitive to polymyxin Experimental - PubMed ID: 17928292
Reference: Escherichia coli K-12 W3110 		Dec 2007
Automated Process Approved note putative transport/receptor protein Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75318.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 9.46
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0005887 integral component of plasma membrane GO Inferred from Sequence or Structural Similarity - PubMed ID: 17928292
Reference: Escherichia coli K-12 W3110 		Dec 2007
Automated Process Uncurated GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000390
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002295 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003103 arnF CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003369 arnF CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003041 arnF CDS
Ortholog
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0003104 arnF CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0003823 arnF CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0009468 CDS
Person Uncurated Go view Dickeya dadantii 3937 ABF-0016240 arnF CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0004267 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0299526 CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0002458 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003276 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005224 CDS
Person Approved Go view Escherichia coli 536 (UPEC) AEF-0002320 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008961 CDS
Person Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002207 yfbJ CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0002064 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006980 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0006687 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0006677 PmrM CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0078938 arnF CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002614 arnF CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0002988 yfbJ CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0006538 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0005876 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0002550 yfbJ CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0006989 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0006738 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005263 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0006705 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007781 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008240 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0005692 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0006025 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0006958 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007308 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0006889 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0006186 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0007480 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027233 yfbJ CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0005098 arnF CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007077 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002833 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002126 yfbJ CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002332 yfbJ CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0002801 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002712 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002647 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0002635 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002559 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0002412 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133157 yfbJ CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0002548 yfbJ CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003889 yfbJ CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0009178 CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0063429 CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0002701 CDS
Automated Process Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0002568 CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0000405 CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0007991 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006608 CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0002697 yfbJ CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0006911 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0007370 CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0007006 CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0006226 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0006905 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006207 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0006639 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0004286 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0006040 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0006850 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0007413 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0000561 CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0000560 CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0000684 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0007092 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0005090 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0006609 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0006584 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0110234 CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0082272 CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0002845 CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0097355 CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0006978 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0004487 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006844 CDS
Person Approved Go view Shigella boydii 227 ADV-0002540 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0005922 CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0002823 CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0073610 CDS
Person Approved Go view Shigella flexneri 301 ABT-0088599 CDS
Person Uncurated Go view Shigella flexneri 8401 BAG-0002497 CDS
Person Uncurated Go view Shigella sonnei 046 ADD-0002538 CDS
Person Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0000574 CDS
Person Uncurated Go view Yersinia enterocolitica 8081 BAH-0006421 CDS
Person Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0000878 arnF CDS
Person Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0001494 CDS
Person Uncurated Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0239546 rhaT8 CDS
Person Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0002128 CDS
Person Uncurated Go view Yersinia pestis CA88-4125 (biovar Orientalis) BAF-0001048 CDS
Person Uncurated Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0123310 pmrM CDS
Person Approved Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0031620 CDS
Person Uncurated Go view Yersinia pestis Nepal516 (biovar Antiqua) BAD-0002253 CDS
Person Uncurated Go view Yersinia pestis Pestoides F (biovar Pestoides) BAI-0000870 CDS
Person Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0005697 CDS
Person Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0002411 pmrM CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22841mutantTn5 InsertionRequest the StrainABE-0007466arnFb2258::Tn5(KAN-I-SceI) at position 438 in Minus orientation ,+ pKD46 0Blattner Laboratory
FB23416mutantTn5 InsertionRequest the StrainABE-0007466arnFb2258::Tn5KAN-I-SceI at position 438 in Minus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2367892 to 2378278
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0007458arnCCDSforward23670712368039Upstream
ABE-0007460arnACDSforward23680392370021Upstream
ABE-0007462arnDCDSforward23700182370908Upstream
ABE-0007464arnTCDSforward23709082372560Upstream
ABE-0285065arnECDSforward23725572372892Overlaps Upstream
ABE-0007466arnFCDSforward23728922373278Matches
ABE-0007468pmrDCDScomplement23732722373538Overlaps Downstream
ABE-0007471menECDScomplement23736482375003Downstream
ABE-0007473menCCDScomplement23750002375962Downstream
ABE-0007476menBCDScomplement23759622376819Downstream
ABE-0007478menHCDScomplement23768342377592Downstream
ABE-0007482menDCDScomplement23775892379259Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.