Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0008012 (yfeW) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0008012 showshow history
Names
yfeW pbp4B JW5395 ECK2425 b2430
Product
penicillin binding protein 4B
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 2548102 2549406 1305 435
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001466 (IPR001466 Beta-lactamase-related) Apr 2008
Automated Process Approved IPR012338 (IPR012338 Beta-lactamase/transpeptidase-like) Apr 2008
Automated Process Approved PF00144 (PF00144 Beta-lactamase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0046677 response to antibiotic GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001466
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0030655 beta-lactam antibiotic catabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001466
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0030288 outer membrane-bounded periplasmic space GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0008800 beta-lactamase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001466
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name yfeW Published Annotation - Swiss-Prot Accession Number: P77619 Aug 2002
Person Approved synonym ECK2425 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym pbp4B Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym JW5395 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2430 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product penicillin binding protein 4B Published Annotation - EcoCyc Accession Number: G7265
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Uncurated alternate product name putative beta-lactamase/D-ala carboxypeptidase Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name putative beta-lactamase Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Approved alternate product name predicted periplasmic esterase Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved alternate product name penicillin binding protein PBP4B; weak DD-carboxypeptidase activity Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Protein Family
Automated Process Approved protein family IPR012338 Beta-lactamase/transpeptidase-like Protein Sequence Similarity - InterPro Domain: IPR012338
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR001466 Beta-lactamase-related Protein Sequence Similarity - InterPro Domain: IPR001466
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00144 Beta-lactamase Protein Sequence Similarity - PFAM Model Name: PF00144
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated EC number 3.-.-.- Protein Sequence Similarity - InterPro Domain: IPR012338
Reference: Escherichia coli K-12 MG1655 		Dec 2008
Person Uncurated MultiFun 1.1.1 (metabolism; carbon utilization; carbon compounds) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0008012 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:87082101 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946907 Published Annotation - Entrez Gene Database ID: 946907
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P77619 Published Annotation - Swiss-Prot Accession Number: P77619 Jan 2006
Automated Process Approved db xref EcoGene:EG14164 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P77619 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved function enzyme; Murein sacculus, peptidoglycan Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved note putative beta-lactamase; GO_process: GO:0016052 - carbohydrate catabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75483.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Periplasmic Predicted by PSORTb - PSORTb Final_Score: 9.76
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0030655 beta-lactam antibiotic catabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001466
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0046677 response to antibiotic GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001466
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0030288 outer membrane-bounded periplasmic space GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0008800 beta-lactamase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001466
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0005655 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003530 yfeW CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0004113 yfeW CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0003882 yfeW CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003652 yfeW CDS
Ortholog
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008794 CDS
Person Uncurated Go view Escherichia coli ATCC 11775 [T] AMC-0005194 yfeW pseudogene
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006803 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0006740 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009480 CDS
Person Uncurated Go view Escherichia coli CFT073 (UPEC) ABR-0081906 yfeW pseudogene
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002776 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0003251 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008493 CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007150 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000773 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007003 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005524 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0004998 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006668 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008500 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0006317 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0006292 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0008151 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007949 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0006631 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0006548 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008075 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027403 yfeW CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007239 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002286 yfeW CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002508 yfeW CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003156 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002875 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002945 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002768 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0002584 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133320 yfeW CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0003620 CDS
Person Uncurated Go view Escherichia coli UTI89 (UPEC) AEA-0005489 yfeW pseudogene
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006790 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0000452 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0002897 yfeW CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0007105 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0007959 CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0007558 CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0006043 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0007099 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006982 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0007084 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006448 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0006223 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0007033 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0008103 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0000392 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0000391 CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0000480 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0007279 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0004903 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0006787 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007437 CDS
Person Uncurated Go view Salmonella Typhi CT18 ABX-0110711 CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0082093 CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0003044 CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0097882 CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0006796 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0004298 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006080 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004361 CDS
Person Approved Go view Shigella sonnei 046 ADD-0002755 yfeW CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22636mutantTn5 InsertionRequest the StrainABE-0008012yfeWb2430::Tn5KAN-I-SceI at position 1373 in Minus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2543102 to 2554406
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0007992cysPCDScomplement25425122543528Upstream
ABE-0007996ucpACDScomplement25438322544623Upstream
ABE-0008000murRCDScomplement25447522545609Upstream
ABE-0008007murQCDSforward25457732546669Upstream
ABE-0008009murPCDSforward25466732548097Upstream
ABE-0008012yfeWCDSforward25481022549406Matches
ABE-0285518repeat_regionforward25494662549563Downstream
ABE-0008015yfeXCDScomplement25496462550545Downstream
ABE-0008017yfeYCDScomplement25506412551216Downstream
ABE-0008023yfeZCDScomplement25512772551726Downstream
ABE-0008028ypeACDScomplement25517132552138Downstream
ABE-0008032amiACDSforward25523522553221Downstream
ABE-0008034hemFCDSforward25532252554124Downstream
ABE-0008036eutRCDScomplement25541302555182Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.