Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0008032 (amiA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0008032 showshow history
Names
amiA yfeE JW2428 ECK2430 b2435
Product
N-acetylmuramoyl-l-alanine amidase I
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 2552352 2553221 870 290
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR006311 (IPR006311 Twin-arginine translocation pathway, signal sequence) Apr 2008
Automated Process Approved IPR002508 (IPR002508 N-acetylmuramoyl-L-alanine amidase, catalytic domain) Apr 2008
Automated Process Approved PF01520 (PF01520 N-acetylmuramoyl-L-alanine amidase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0009252 peptidoglycan biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0009253 peptidoglycan catabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002508
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0008745 N-acetylmuramoyl-L-alanine amidase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002508
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name amiA Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK2430 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW2428 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym yfeE Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved locus tag b2435 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product N-acetylmuramoyl-l-alanine amidase I Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name N-acetylmuramoyl-l-alanine amidase I Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR006311 Twin-arginine translocation pathway, signal sequence Protein Sequence Similarity - InterPro Domain: IPR006311
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR002508 N-acetylmuramoyl-L-alanine amidase, catalytic domain Protein Sequence Similarity - InterPro Domain: IPR002508
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF01520 N-acetylmuramoyl-L-alanine amidase Protein Sequence Similarity - PFAM Model Name: PF01520
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 3.5.1.28 Published Annotation - Swiss-Prot Accession Number: P36548 Feb 2006
Person Uncurated MultiFun 6.2 (cell structure; peptidoglycan (murein)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.6.7 (metabolism; macromolecules (cellular constituent) biosynthesis; peptidoglycan (murein)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11823 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P36548 Published Annotation - Swiss-Prot Accession Number: P36548 Jan 2005
Automated Process Approved db xref GeneID:946916 Published Annotation - Entrez Gene Database ID: 946916
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:1788776 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P36548 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0008032 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Murein sacculus, peptidoglycan Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_process: GO:0009252 - peptidoglycan biosynthetic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75488.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.50
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0009253 peptidoglycan catabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002508
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0009252 peptidoglycan biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0008745 N-acetylmuramoyl-L-alanine amidase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002508
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0006804 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002494 amiA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002483 amiA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002275 amiA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002439 amiA CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0003193 amiA CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0000890 CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0003281 amiA CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0003963 amiA CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0003893 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0303645 amiA CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC BAA-2158 APN-0002695 amiA CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0001131 amiA CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0003087 amiA CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006821 amiA CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002476 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008788 amiA CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002397 amiA CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001863 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006796 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0006745 amiA CDS
Person Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009485 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0079113 amiA CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002781 amiA CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0003256 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008498 amiA CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0002777 amiA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007155 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000778 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007008 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005529 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0005003 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006663 amiA CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008505 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0006312 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0006297 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0008156 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007954 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0006636 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0006553 amiA CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008080 amiA CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027408 amiA CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0008033 amiA CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007245 amiA CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0002983 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002291 amiA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002513 amiA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003151 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002880 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002940 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0002799 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002773 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0002589 amiA CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133325 amiA CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0003625 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0002776 amiA CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0002552 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0002787 amiA CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0001127 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0002142 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0003506 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0002102 amiA CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0001910 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0002856 amiA CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0001286 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0010592 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0001081 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0290918 amiA CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0005975 amiA CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006763 amiA CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0000447 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0002864 amiA CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0007077 amiA CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0007881 amiA CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0007485 amiA CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0006070 amiA CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0007072 amiA CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006954 amiA CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0007109 amiA CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006420 amiA CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0006195 amiA CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0007006 amiA CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0008131 amiA CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0000417 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0000416 amiA CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0000510 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0007251 amiA CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0004930 amiA CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0006762 amiA CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007462 amiA CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0110642 amiA CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0082120 amiA CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0003013 amiA CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0097802 amiA CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0006822 amiA CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0004325 amiA CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006051 amiA CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0002239 CDS
Person Approved Go view Shigella boydii 227 ADV-0002716 amiA CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0008216 amiA CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004366 amiA CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003018 amiA CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0073785 amiA CDS
Person Approved Go view Shigella flexneri 301 ABT-0088747 amiA CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0002659 amiA CDS
Person Approved Go view Shigella sonnei 046 ADD-0002760 amiA CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0017713 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0001855 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20798mutantTn5 InsertionRequest the StrainABE-0008032amiAamiA::Tn5KAN-2 at position 464 in Plus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2547352 to 2558221
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0008009murPCDSforward25466732548097Upstream
ABE-0008012yfeWCDSforward25481022549406Upstream
ABE-0285518repeat_regionforward25494662549563Upstream
ABE-0008015yfeXCDScomplement25496462550545Upstream
ABE-0008017yfeYCDScomplement25506412551216Upstream
ABE-0008023yfeZCDScomplement25512772551726Upstream
ABE-0008028ypeACDScomplement25517132552138Upstream
ABE-0008032amiACDSforward25523522553221Matches
ABE-0008034hemFCDSforward25532252554124Downstream
ABE-0008036eutRCDScomplement25541302555182Downstream
ABE-0285519repeat_regionforward25551922555215Downstream
ABE-0008039eutKCDScomplement25552282555728Downstream
ABE-0008041eutLCDScomplement25557412556400Downstream
ABE-0008043eutCCDScomplement25564102557297Downstream
ABE-0008046eutBCDScomplement25573182558679Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.