Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0008183 (hyfC) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0008183 showshow history
Names
hyfC JW2468 ECK2479 b2483
Product
hydrogenase 4 component C
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 2603847 2604794 948 316
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001694 (IPR001694 NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H) Apr 2008
Automated Process Approved PF00146 (PF00146 NADH dehydrogenase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001694
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001694
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001694
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name hyfC Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW2468 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK2479 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2483 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product hydrogenase 4 component C Published Annotation - EcoCyc Accession Number: G7300
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Approved alternate product name hydrogenase 4 membrane subunit Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name hydrogenase 4, C (membrane) subunit Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name hydrogenase 4, membrane subunit Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family PF00146 NADH dehydrogenase Protein Sequence Similarity - PFAM Model Name: PF00146
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR001694 NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H Protein Sequence Similarity - InterPro Domain: IPR001694
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Approved EC number 1.-.-.- Published Annotation - Swiss-Prot Accession Number: P77858 Feb 2006
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG14211 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:87082115 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:947492 Published Annotation - Entrez Gene Database ID: 947492
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P77858 Published Annotation - Swiss-Prot Accession Number: P77858 Jan 2005
Automated Process Approved db xref UniProtKB/Swiss-Prot:P77858 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0008183 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Energy metabolism, carbon: Anaerobic respiration Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note hydrogenase 4 membrane subunit; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75536.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001694
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001694
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001694
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002656 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003960 hyfC CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0001575 hyfC CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004150 hyfC CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003622 hyfC CDS
Ortholog
Automated Process Uncurated Go view Brenneria salicis ATCC 15712 [T] AMK-0003725 CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0007620 CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0015737 hyfC CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0002697 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008744 hyfC CDS
Person Uncurated Go view Escherichia coli ATCC 8739 AEM-0006742 hyfC pseudogene
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0006789 hyfC CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0007911 CDS
Person Uncurated Go view Escherichia coli BL21(DE3) AHL-0001983 pseudogene
Person Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002829 pseudogene
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0003318 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0006753 hyfC CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008541 hyfC CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007199 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000823 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007048 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005569 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0005043 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006623 hyfC CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008545 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0006272 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0006337 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0008196 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007994 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0006676 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0006593 hyfC CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008121 hyfC CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027449 hyfC CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007288 hyfC CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002339 hyfC CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002553 hyfC CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003106 hycD CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0002924 hycD CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002899 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002815 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0002629 hyfC CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133364 hyfC CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0003670 CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0003149 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0005877 CDS
Person Approved Go view Pectobacterium atrosepticum SCRI1043 ABL-0061491 hyfC CDS
Person Approved Go view Shigella boydii 227 ADV-0002764 hyfC CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0008263 hyfC CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003062 hyfC CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0073827 hyfC CDS
Person Approved Go view Shigella flexneri 301 ABT-0256070 hyfC CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0002703 hyfC CDS
Person Approved Go view Shigella sonnei 046 ADD-0002800 hyfC CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0001151 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0008058 hyfC CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0002225 hyfC CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0001336 hyfC CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0003596 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0008222 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0002191 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22429mutantTn5 InsertionRequest the StrainABE-0008183hyfChyfC::Tn5KAN-I-SceI at position 597 in Plus orientation ,+ pKD460Blattner Laboratory
FB23575mutantTn5 InsertionRequest the StrainABE-0008183hyfChyfC::Tn5KAN-I-SceI at position 597 in Plus orientation ,- pKD460Blattner Laboratory
FB23699mutantTn5 InsertionRequest the StrainABE-0008183hyfChyfC::Tn5KAN-I-SceI at position 597 in Plus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2598847 to 2609794
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0008161bamCCDScomplement25978312598865Upstream
ABE-0008163dapACDScomplement25988822599760Upstream
ABE-0008168gcvRCDSforward25999062600478Upstream
ABE-0008171bcpCDSforward26004782600948Upstream
ABE-0008179hyfACDSforward26012012601818Upstream
ABE-0008181hyfBCDSforward26018182603836Upstream
ABE-0008183hyfCCDSforward26038472604794Matches
ABE-0008185hyfDCDSforward26048112606250Downstream
ABE-0008188hyfECDSforward26062622606912Downstream
ABE-0008191hyfFCDSforward26069172608497Downstream
ABE-0008194hyfGCDSforward26084872610154Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.