Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0008206 (focB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0008206 showshow history
Names
focB JW2477 ECK2488 b2492
Product
formate channel protein
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 2613934 2614782 849 283
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000292 (IPR000292 Formate/nitrite transporter) Apr 2008
Automated Process Approved PF01226 (PF01226 Formate/nitrite transporter) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000292
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000292
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000292
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0022857 transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000292
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000292
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name focB Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW2477 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK2488 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2492 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product formate channel protein Published Annotation - EcoCyc Accession Number: G7309
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Approved alternate product name probable formate transporter (formate channel 2) Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name putative formate transport protein (formate channel 2) (FNT family) Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name predicted formate transporter Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family IPR000292 Formate/nitrite transporter Protein Sequence Similarity - InterPro Domain: IPR000292
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF01226 Formate/nitrite transporter Protein Sequence Similarity - PFAM Model Name: PF01226
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.2.A.44 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Formate-Nitrite Transporter (FNT) Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.59 (transport; substrate; formate) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0008206 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG14220 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P77733 Published Annotation - Swiss-Prot Accession Number: P77733 Jan 2005
Automated Process Approved db xref GI:1788837 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:949032 Published Annotation - Entrez Gene Database ID: 949032
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref UniProtKB/Swiss-Prot:P77733 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function putative transport; Transport of small molecules: Carbohydrates, organic acids, alcohols Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note probable formate transporter (formate channel 2); GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75545.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000292
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000292
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000292
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000292
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0022857 transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000292
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0004631 focB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0005491 focB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003942 focB CDS
Ortholog
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006771 fnt CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008736 fnt CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006730 CDS
Person Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009753 pseudogene
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002841 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0003328 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0006744 fnt CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007208 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000832 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007057 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005578 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0005052 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006614 fnt CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008554 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0006263 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0006346 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0008205 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008003 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0006685 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0006602 fnt CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008130 fnt CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027458 focB CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007297 fnt CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002348 focB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002562 focB CDS
Automated Process Uncurated Go view Escherichia coli SE11 AFS-0002825 CDS
Automated Process Uncurated Go view Escherichia coli SMS-3-5 AFT-0002638 fnt CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133373 focB CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0003679 CDS
Automated Process Uncurated Go view Shigella boydii BS512 ADJ-0008272 fnt CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004416 CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003071 focB CDS
Person Uncurated Go view Shigella flexneri 2457T ABQ-0073837 focB pseudogene
Person Uncurated Go view Shigella flexneri 301 ABT-0088792 focB pseudogene
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22437mutantTn5 InsertionRequest the StrainABE-0008206focBfocB::Tn5KAN-I-SceI at position 807 in Minus orientation ,+ pKD460Blattner Laboratory
FB23582mutantTn5 InsertionRequest the StrainABE-0008206focBfocB::Tn5KAN-I-SceI at position 807 in Minus orientation ,- pKD460Blattner Laboratory
FB23706mutantTn5 InsertionRequest the StrainABE-0008206focBfocB::Tn5KAN-I-SceI at position 807 in Minus orientation ,- pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2608934 to 2619782
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0008194hyfGCDSforward26084872610154Upstream
ABE-0008196hyfHCDSforward26101642610709Upstream
ABE-0008198hyfICDSforward26107062611464Upstream
ABE-0008200hyfJCDSforward26114572611870Upstream
ABE-0008204hyfRCDSforward26119002613912Upstream
ABE-0008206focBCDSforward26139342614782Matches
ABE-0008208yfgOCDScomplement26148202615881Downstream
ABE-0008212bepACDSforward26160942617557Downstream
ABE-0008214yfgDCDSforward26175782617937Downstream
ABE-0285525repeat_regionforward26179682618004Downstream
ABE-0008217hdaCDScomplement26180752618776Downstream
ABE-0008219uraACDScomplement26188712620160Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.