Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0008487 (eamB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0008487 showshow history
Names
eamB JW2562 ECK2576 yfiK b2578
Product
cysteine and O-acetylserine exporter
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 2715423 2716010 588 196
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001123 (IPR001123 Amino acid exporter protein, LeuE-type) Apr 2008
Automated Process Approved PF01810 (PF01810 LysE type translocator) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006865 amino acid transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001123
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001123
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0005293 (DEPRECATED) lysine permease activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001123
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Uncurated name eamB Published Annotation in EcoGene - EG Number: EG12445
Reference: Escherichia coli K-12 MG1655 		Apr 2009
Person Approved synonym yfiK Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW2562 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK2576 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2578 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product cysteine and O-acetylserine exporter Experimental - PubMed ID: 12562784
Reference: Escherichia coli K-12 		Apr 2009
Person Uncurated alternate product name neutral amino-acid efflux system Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 		Apr 2009
Protein Family
Automated Process Approved protein family PF01810 LysE type translocator Protein Sequence Similarity - PFAM Model Name: PF01810
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR001123 Amino acid exporter protein, LeuE-type Protein Sequence Similarity - InterPro Domain: IPR001123
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 4.9.B (transport; Transporters of Unknown Classification; Putative uncharacterized transport protein) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12445 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0008487 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1788932 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P38101 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:947065 Published Annotation - Entrez Gene Database ID: 947065
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P38101 Published Annotation - Swiss-Prot Accession Number: P38101 Jan 2005
Automated Process Approved protein id AAC75631.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006865 amino acid transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001123
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001123
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0005293 (DEPRECATED) lysine permease activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001123
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0000124 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0001742 eamB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002027 eamB CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000352 eamB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002091 eamB CDS
Ortholog
Person Uncurated Go view Citrobacter freundii ATCC 8090 [T] (AToL DRAFT) API-0005040 eamB pseudogene
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0002959 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006672 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002600 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008560 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002557 yfiK CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001737 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006631 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0008988 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0008294 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0079245 eamB CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0002941 eamB CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0003420 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0006646 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0007799 CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007299 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000927 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007158 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005677 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0005152 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006513 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008654 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0006164 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0006446 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0009644 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008103 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0010589 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0009405 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0010639 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027552 yfiK CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0009259 eamB CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007410 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003016 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002433 yfiK CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002650 yfiK CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0002946 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003030 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0002996 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0002924 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177107 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0002915 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0002730 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133463 yfiK CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0003776 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0002908 yfiK CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0000234 CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0006238 CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0003510 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0006922 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0003100 yfiK CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0007234 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0008396 yfiK CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0007967 yfiK CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0005916 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0007222 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0008661 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0006932 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006586 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0006363 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0007219 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0009255 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0000275 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0000273 yfiK CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0000336 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0007401 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0008703 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0006913 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007556 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0111051 yfiK CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0081977 yfiK CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0003247 yfiK CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0098361 yfiK CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0006672 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0009296 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0008900 CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0265282 CDS
Person Approved Go view Shigella boydii 227 ADV-0002877 yfiK CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0008371 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004533 CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0073967 yfiK CDS
Person Approved Go view Shigella flexneri 301 ABT-0088890 yfiK CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0002821 yfiK CDS
Person Approved Go view Shigella sonnei 046 ADD-0002957 yfiK CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22739mutantTn5 InsertionRequest the StrainABE-0008487eamByfiK::Tn5KAN-I-SceI at position 379 in Plus orientation ,+ pKD460Blattner Laboratory
FB23180mutantTn5 InsertionRequest the StrainABE-0008487eamByfiK::Tn5KAN-I-SceI at position 379 in Plus orientation ,- pKD465Tim Durfee (durf@genome.wisc.edu)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2710423 to 2721010
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0008471nadBCDSforward27104202712042Upstream
ABE-0008473yfiCCDScomplement27120272712764Upstream
ABE-0008478srmBCDSforward27128962714230Upstream
ABE-0285531repeat_regionforward27142832714380Upstream
ABE-0008483yfiECDScomplement27144392715320Upstream
ABE-0008487eamBCDSforward27154232716010Matches
ABE-0008489grcACDScomplement27160662716449Downstream
ABE-0008495ungCDSforward27167542717443Downstream
ABE-0285532repeat_regionforward27174582717483Downstream
ABE-0008497yfiFCDScomplement27174912718528Downstream
ABE-0008501trxCCDSforward27187352719154Downstream
ABE-0008503tapTCDSforward27192232719921Downstream
ABE-0008506patZCDSforward27199532722613Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.