Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0008926 (ascF) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0008926 showshow history
Names
ascF JW5435 ECK2710 sac b2715
Product
PTS system arbutin/salicin/cellobiose-specific enzyme II component
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 2839524 2840981 1458 486
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001996 (IPR001996 Phosphotransferase system, IIB component, type 1) Apr 2008
Automated Process Approved IPR011535 (IPR011535 (DEPRECATED) Phosphotransferase system, glucose-like IIB component) Apr 2008
Automated Process Approved IPR004719 (IPR004719 Phosphotransferase system, maltose/glucose-specific subfamily IIC component) Apr 2008
Automated Process Approved IPR013013 (IPR013013 Phosphotransferase system, EIIC component, type 1) Apr 2008
Automated Process Approved IPR003352 (IPR003352 Phosphotransferase system, EIIC) Apr 2008
Automated Process Approved IPR018113 (IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site) Jul 2018
Automated Process Approved IPR036878 (IPR036878 Glucose permease domain IIB) Jul 2018
Automated Process Approved PF02378 (PF02378 Phosphotransferase system, EIIC) Jul 2018
Automated Process Approved PF00367 (PF00367 phosphotransferase system, EIIB) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0009401 phosphoenolpyruvate-dependent sugar phosphotransferase system GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001996
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001996
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004719
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001996
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0008982 protein-N(PI)-phosphohistidine-sugar phosphotransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003352, IPR018113, IPR004719, IPR036878
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0005351 carbohydrate:proton symporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001996
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name ascF Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym sac Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved synonym ECK2710 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW5435 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2715 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product PTS system arbutin/salicin/cellobiose-specific enzyme II component Experimental - PubMed ID: 1630307
Reference: Escherichia coli K-12 		Mar 2008
Automated Process Approved alternate product name fused cellobiose/arbutin/salicin-specific PTS enzymes: IIB component/IC component Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Uncurated alternate product name PTS family enzyme IIBC component, cellobiose/salicin/arbutin-specific Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name PTS system enzyme II ABC (asc), cryptic, transports specific beta-glucosides Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site Protein Sequence Similarity - InterPro Domain: IPR018113
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR001996 Phosphotransferase system, IIB component, type 1 Protein Sequence Similarity - InterPro Domain: IPR001996
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR011535 (DEPRECATED) Phosphotransferase system, glucose-like IIB component Protein Sequence Similarity - InterPro Domain: IPR011535
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR004719 Phosphotransferase system, maltose/glucose-specific subfamily IIC component Protein Sequence Similarity - InterPro Domain: IPR004719
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR003352 Phosphotransferase system, EIIC Protein Sequence Similarity - InterPro Domain: IPR003352
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR036878 Glucose permease domain IIB Protein Sequence Similarity - InterPro Domain: IPR036878
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR013013 Phosphotransferase system, EIIC component, type 1 Protein Sequence Similarity - InterPro Domain: IPR013013
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF02378 Phosphotransferase system, EIIC Protein Sequence Similarity - PFAM Model Name: PF02378
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00367 phosphotransferase system, EIIB Protein Sequence Similarity - PFAM Model Name: PF00367
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Approved EC number 2.7.1.69 Published Annotation - Swiss-Prot Accession Number: P24241 Feb 2006
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 10 (cryptic genes) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.29 (transport; substrate; cellobiose/arbutin/salicin) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.1.1 (metabolism; carbon utilization; carbon compounds) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.4.A.1 (transport; Group Translocators; Phosphotransferase Systems (PEP-dependent PTS); The PTS Glucose-Glucoside (Glc) Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:48994906 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P24241 Published Annotation - Swiss-Prot Accession Number: P24241 Jan 2005
Automated Process Approved db xref ASAP:ABE-0008926 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:947154 Published Annotation - Entrez Gene Database ID: 947154
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref UniProtKB/Swiss-Prot:P24241 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG10086 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note PTS system enzyme II ABC (asc), cryptic, transports specific beta-glucosides; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0016052 - carbohydrate catabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAT48150.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved sequence correction version m56: H167 to A, L168 to S, P169 to A, R170 to A Experimental - GenBank Accession Number: AG613245
Reference: Escherichia coli 		May 2004
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001996
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0009401 phosphoenolpyruvate-dependent sugar phosphotransferase system GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001996
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR004719
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001996
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0008982 protein-N(PI)-phosphohistidine-sugar phosphotransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR003352, IPR018113, IPR004719, IPR036878
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0005351 carbohydrate:proton symporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001996
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0003224 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003286 ascF CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002881 ascF CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002938 ascF CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003096 ascF CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0003779 CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0001183 CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0003538 bglF_1 CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0004266 bglF CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0004949 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0008272 ascF CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002705 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008448 ascF CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002713 ascF CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001580 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006506 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0007416 ascF CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0007021 CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003098 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008167 ascF CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008817 ascF CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007437 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001282 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007305 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005826 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0005301 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0008072 ascF CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008802 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007396 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0006596 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007420 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008238 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0008471 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007920 ascF CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008273 ascF CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027693 ascF CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0006358 bglF_1 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007526 ascF CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002544 ascF CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002784 ascF CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003279 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003195 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003145 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177277 pseudogene
Automated Process Approved Go view Escherichia coli SE11 AFS-0003020 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0002837 ascF CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133607 ascF CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0000863 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003086 ascF CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0001912 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003060 ascF CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0001428 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0006534 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0004059 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0000867 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0003954 ascF CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0004699 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0004340 ascF CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0004328 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0007756 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0002224 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0286581 ascF CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0008360 ascF CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0062427 ascF CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0001244 ascF CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0000553 ascF CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0003705 ascF CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0007049 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0000434 CDS
Person Approved Go view Shigella boydii 227 ADV-0003083 ascF CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0005539 CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0002981 ascF CDS
Person Approved Go view Shigella sonnei 046 ADD-0003128 ascF CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0001778 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0003748 ascF CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0000712 ascF CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0001065 ascF CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0001083 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0008254 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0002180 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2834524 to 2845981
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0008912norWCDSforward28339122835045Upstream
ABE-0285539repeat_regionforward28350672835148Upstream
ABE-0008915hypFCDScomplement28351732837425Upstream
ABE-0285540repeat_regionforward28374652837491Upstream
ABE-0008919hydNCDScomplement28375782838105Upstream
ABE-0008922ascGCDScomplement28382542839264Upstream
ABE-0008926ascFCDSforward28395242840981Matches
ABE-0008928ascBCDSforward28409902842414Downstream
ABE-0285541repeat_regionforward28424232842501Downstream
ABE-0008931hycICDScomplement28425732843043Downstream
ABE-0008933hycHCDScomplement28430362843446Downstream
ABE-0008936hycGCDScomplement28434432844210Downstream
ABE-0008942hycFCDScomplement28442102844752Downstream
ABE-0008944hycECDScomplement28447622846471Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.