Escherichia coli K-12 MG1655
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0008936 (hycG) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0008936 showshow history
Names
hycG hevG JW2689 ECK2714 b2719
Product
formate hydrogenlyase subunit
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2843443 2844210 768 256
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR014406 (IPR014406 (DEPRECATED) [NiFe]-hydrogenase-3-type complex, small subunit/NADH:quinone oxidoreductase, subunit NuoB) Apr 2008
Automated Process Approved IPR014658 (IPR014658 (DEPRECATED) [NiFe]-hydrogenase-3-type complex, small subunit) Apr 2008
Automated Process Approved IPR006138 (IPR006138 NADH-ubiquinone oxidoreductase, 20 Kd subunit) Apr 2008
Automated Process Approved IPR006137 (IPR006137 NADH:ubiquinone oxidoreductase-like, 20kDa subunit) Apr 2008
Automated Process Approved PF01058 (PF01058 NADH ubiquinone oxidoreductase, 20 Kd subunit) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0006113 fermentation Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006137
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR014406
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006137
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO molecular function GO:0051540 metal cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR014658
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO molecular function GO:0051539 4 iron, 4 sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR014406
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO molecular function GO:0016651 oxidoreductase activity, acting on NAD(P)H GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR014406
Reference: Escherichia coli K-12 MG1655
Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name hycG Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Person Approved synonym ECK2714 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW2689 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym hevG Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved locus tag b2719 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product formate hydrogenlyase subunit Published Annotation - EcoCyc Accession Number: EG10480
Reference: Escherichia coli K-12 MG1655
Oct 2021
Person Approved alternate product name hydrogenase activity Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Jan 2006
Person Uncurated alternate product name subunit of hydrogenase 3 and formate hydrogenase complex Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name hydrogenase 3 and formate hydrogenase complex, HycG subunit Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family IPR006137 NADH:ubiquinone oxidoreductase-like, 20kDa subunit Protein Sequence Similarity - InterPro Domain: IPR006137
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved protein family PF01058 NADH ubiquinone oxidoreductase, 20 Kd subunit Protein Sequence Similarity - PFAM Model Name: PF01058
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR006138 NADH-ubiquinone oxidoreductase, 20 Kd subunit Protein Sequence Similarity - InterPro Domain: IPR006138
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved protein family IPR014658 (DEPRECATED) [NiFe]-hydrogenase-3-type complex, small subunit Protein Sequence Similarity - InterPro Domain: IPR014658
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved protein family IPR014406 (DEPRECATED) [NiFe]-hydrogenase-3-type complex, small subunit/NADH:quinone oxidoreductase, subunit NuoB Protein Sequence Similarity - InterPro Domain: IPR014406
Reference: Escherichia coli K-12 MG1655
Apr 2008
Other
Automated Process Approved EC number 1.12.7.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Uncurated EC number 1.6.-.- Protein Sequence Similarity - InterPro Domain: IPR006138
Reference: Escherichia coli K-12 MG1655
Dec 2008
Person Uncurated MultiFun 1.3.5 (metabolism; energy metabolism, carbon; fermentation) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved db xref UniProtKB:P16433 Published Annotation - Swiss-Prot Accession Number: P16433 Jan 2005
Automated Process Approved db xref GI:1789074 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GeneID:947191 Published Annotation - Entrez Gene Database ID: 947191
Reference: Escherichia coli K-12 MG1655
Jun 2009
Automated Process Approved db xref UniProtKB/Swiss-Prot:P16433 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref ASAP:ABE-0008936 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref EcoGene:EG10480 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved function phenotype; Energy metabolism, carbon: Fermentation Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Automated Process Approved note hydrogenase activity; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006113 - fermentation; GO_process: GO:0009061 - anaerobic respiration Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved protein id AAC75761.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655
Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655
Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655
Mar 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006137
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR014406
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO biological process GO:0006113 fermentation Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0051540 metal cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR014658
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006137
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO molecular function GO:0051539 4 iron, 4 sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR014406
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO molecular function GO:0016651 oxidoreductase activity, acting on NAD(P)H GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR014406
Reference: Escherichia coli K-12 MG1655
Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002657 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002792 hycG CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0001823 hyfI CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004149 hyfI CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002683 hycG CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0004196 hycG CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0004208 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0003543 hycG CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0004271 hycG CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0004945 CDS
Person Approved Go view Enterobacter sp. 638 AEL-0003891 hycG CDS
Automated Process Approved Go view Escherichia albertii TW07627 AFX-0002833 hycG CDS
Automated Process Approved Go view Escherichia coli 101-1 (EAEC) BAB-0008268 hycG CDS
Person Approved Go view Escherichia coli 536 (UPEC) AEF-0002711 hycG CDS
Automated Process Approved Go view Escherichia coli 53638 (EIEC) ADP-0008444 CDS
Person Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002718 hycG CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001574 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006502 CDS
Automated Process Approved Go view Escherichia coli B171 (EPEC) ADN-0007422 hycG CDS
Automated Process Approved Go view Escherichia coli B7A (ETEC) ADK-0007017 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0079435 hycG CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003106 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0003614 CDS
Automated Process Approved Go view Escherichia coli E110019 (EPEC) ADM-0008161 hycG CDS
Automated Process Approved Go view Escherichia coli E22 (EPEC) ADL-0008823 hycG CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0003177 hycG CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007444 hycG CDS
Automated Process Approved Go view Escherichia coli EC4024 (EHEC) BBI-0001286 CDS
Automated Process Approved Go view Escherichia coli EC4042 (EHEC) BBA-0007309 hycG CDS
Automated Process Approved Go view Escherichia coli EC4045 (EHEC) BBB-0005830 hycG CDS
Automated Process Approved Go view Escherichia coli EC4076 (EHEC) BAZ-0005305 hycG CDS
Automated Process Approved Go view Escherichia coli EC4113 (EHEC) BAY-0008068 hycG CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008806 hycG CDS
Automated Process Approved Go view Escherichia coli EC4196 (EHEC) BAX-0007392 hycG CDS
Automated Process Approved Go view Escherichia coli EC4206 (EHEC) BAW-0006600 hycG CDS
Automated Process Approved Go view Escherichia coli EC4401 (EHEC) BAV-0007424 hycG CDS
Automated Process Approved Go view Escherichia coli EC4486 (EHEC) BAU-0008242 hycG CDS
Automated Process Approved Go view Escherichia coli EC4501 (EHEC) BAT-0008467 hycG CDS
Automated Process Approved Go view Escherichia coli EC508 (EHEC) BBJ-0007916 hycG CDS
Automated Process Approved Go view Escherichia coli EC869 (EHEC) BBC-0008277 hycG CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027697 hycG CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0006364 hycG CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007530 hycG CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003201 CDS
Person Approved Go view Escherichia coli K-12 DH10B AEC-0002548 hycG CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002788 hycG CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003574 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003202 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003141 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003035 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177282 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003024 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0002842 hycG CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133611 hycG CDS
Automated Process Approved Go view Escherichia coli TW14588 (EHEC) BBK-0000867 CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003091 hycG CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0001496 CDS
Person Approved Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003065 hycG CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0007659 CDS
Automated Process Approved Go view Salmonella Agona SL483 BBX-0007135 hycG CDS
Automated Process Approved Go view Salmonella Arizonae CDC 346-86 BCD-0000128 CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0003257 hycG CDS
Automated Process Approved Go view Salmonella Dublin CT_02021853 BCB-0007413 hycG CDS
Automated Process Approved Go view Salmonella Enteritidis P125109 BCF-0008790 hycG CDS
Automated Process Approved Go view Salmonella Gallinarum 287/91 BCG-0008338 hycG CDS
Automated Process Approved Go view Salmonella Hadar RI_05P066 BBT-0006555 hycG CDS
Automated Process Approved Go view Salmonella Heidelberg SL476 BBV-0007394 hycG CDS
Automated Process Approved Go view Salmonella Heidelberg SL486 BBW-0006352 hycG CDS
Automated Process Approved Go view Salmonella Javiana GA_MM04042433 BCA-0005392 hycG CDS
Automated Process Approved Go view Salmonella Kentucky CDC 191 BBZ-0005409 hycG CDS
Automated Process Approved Go view Salmonella Kentucky CVM29188 BBP-0006517 hycG CDS
Automated Process Approved Go view Salmonella Newport SL254 BBQ-0007409 hycG CDS
Automated Process Approved Go view Salmonella Newport SL317 BBR-0005650 hycG CDS
Automated Process Approved Go view Salmonella Paratyphi A AKU_12601 BCH-0002733 CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0002704 hycG CDS
Automated Process Approved Go view Salmonella Paratyphi B SPB7 BCC-0003556 CDS
Automated Process Approved Go view Salmonella Saintpaul SARA23 BBN-0007573 hycG CDS
Automated Process Approved Go view Salmonella Saintpaul SARA29 BBM-0005651 hycG CDS
Automated Process Approved Go view Salmonella Schwarzengrund CVM19633 BBO-0007071 hycG CDS
Automated Process Approved Go view Salmonella Schwarzengrund SL480 BBY-0005280 hycG CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0111390 hycG CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0084466 hycG CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0003446 hycG CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0098916 hycG CDS
Automated Process Approved Go view Salmonella Virchow SL491 BBS-0006451 hycG CDS
Automated Process Approved Go view Salmonella Weltevreden SL484 BBU-0006796 hycG CDS
Automated Process Approved Go view Salmonella enterica CVM23701 BBL-0008225 hycG CDS
Person Approved Go view Serratia proteamaculans 568 AEN-0008961 hycG CDS
Person Approved Go view Shigella boydii 227 ADV-0003079 hycG CDS
Automated Process Approved Go view Shigella dysenteriae 1012 BAA-0005532 hycG CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003335 hycG pseudogene
Person Approved Go view Shigella flexneri 8401 BAG-0002975 hycG CDS
Person Approved Go view Shigella sonnei 046 ADD-0003135 hycG CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)

Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20899mutantTn5 InsertionRequest the StrainABE-0008936hycGhycG::Tn5KAN-I-SceI at position 551 in Minus orientation ,- pKD465Blattner Laboratory
FB20900mutantTn5 InsertionRequest the StrainABE-0008936hycGhycG::Tn5KAN-I-SceI at position 551 in Minus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2838443 to 2849210
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0008922ascGCDScomplement28382542839264Downstream
ABE-0008926ascFCDSforward28395242840981Downstream
ABE-0008928ascBCDSforward28409902842414Downstream
ABE-0285541repeat_regionforward28424232842501Downstream
ABE-0008931hycICDScomplement28425732843043Downstream
ABE-0008933hycHCDScomplement28430362843446Overlaps Downstream
ABE-0008936hycGCDScomplement28434432844210Matches
ABE-0008942hycFCDScomplement28442102844752Overlaps Upstream
ABE-0008944hycECDScomplement28447622846471Upstream
ABE-0008946hycDCDScomplement28464892847412Upstream
ABE-0008948hycCCDScomplement28474152849241Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.